Rapd
Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines
Authors: A.A. Leal, C.A. Mangolin, A.T. do Amaral J�ºnior, L.S.A. Gon�§alves, C.A. Scapim, A.S. Mott, I.B.O. Eloi, V. Cordov�©s and M.F.P. da Silva
Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S7 inbred popcorn lines using .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr692
- DOI:
- 10.4238/vol9-1gmr692
RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas
Authors: J.C.V. Guerra Jr., M.R.C. Issa, F.E. Carneiro, R. Strapazzon and G. Moretto
The mite Varroa destructor is the main pest causing damage to apiculture worldwide. In Brazil and other parts of the world, where bees of African origin and their hybrids predominate, the bees can survive these mites without treatment. Studies have shown a correlation between the various genotypes of the mite and its fertility.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr696
- DOI:
- 10.4238/vol9-1gmr696
Optimization of DNA extraction from seeds and fresh leaf tissues of wild marigold (Tagetes minuta) for polymerase chain reaction analysis
Authors: I. Shahzadi, R. Ahmed, A. Hassan and M.M. Shah
Tagetes, a genus of flowering marigolds in the family Asteraceae (Compositeae), is reported to be a medicinal plant with hypotensive, spasmolytic, anti-inflammatory, antimicrobial, and antifungal properties. Tagetes minuta characteristically contains high concentrations of essential oils, flavonoids, polyphenols, and polysacch.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr747
- DOI:
- 10.4238/vol9-1gmr747
DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA
Authors: S. Tabasum, F.A. Khan, S. Nawaz, M.Z. Iqbal and A. Saeed
DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr709
- DOI:
- 10.4238/vol9-1gmr709
Genetic diversity of Palestine landraces of faba bean (Vicia faba) based on RAPD markers
Authors: R. Basheer-Salimia, M. Shtaya, M. Awad, J. Abdallah and Y. Hamdan
Until now, neither phenotypic nor molecular approaches have been used to characterize the landraces of Palestine faba beans (Vicia faba). We used PCR-based RAPD markers to determine the genetic diversity and relatedness among 26 Palestinian faba bean landraces (traditional farmers’ varieties) from 8 localities in the West Bank, Palestine. In tests w.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.3.8
- DOI:
- 10.4238/2013.September.3.8
Genetic relationships between Lolium (Poaceae) species revealed by RAPD markers
Authors: X. Ma, X.-Y. Gu, T.-T. Chen, S.-Y. Chen, L.-K. Huang and X.-Q. Zhang
The genus Lolium is one of the most important groupings of temperate forage grasses, including about eight recognized species that are native to some temperate and subtropical regions of the northern hemisphere. We examined genetic relationships among 18 accessions representing all Lolium species using RAPD markers. Among 50 random primers that we screene.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.March.11.4
- DOI:
- 10.4238/2013.March.11.4
Characterization and genetic diversity of pepper (Capsicum spp) parents and interspecific hybrids
Authors: M.P.S.D. Costa, M.M. do R�ªgo, A.P.G. da Silva, E.R. do R�ªgo and P.A. Barroso
Pepper species exhibit broad genetic diversity, which enables their use in breeding programs. The objective of this study was to characterize the diversity between the parents of different species and their interspecific hybrids using morphological and molecular markers. The parents of Capsicum annuum (UFPB-01 and -137), C. ba.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027652
- DOI:
- 10.4238/gmr.15027652
Identification of a locus characteristic of male individuals of buffalo grass [Buchloe dactyloides (Nutt.) Engelm.] by using an RAPD marker
Authors: Y.X. Li, X.G. Wang, C.H. Yang, L.L. Cong, F.F. Wu, J.G. Xue and Y.H. Han
Buffalo grass [Buchloe dactyuloides (Nutt.) Engelm.] plants can be either male, female, or hermaphrodite (monoecious). As there is no morphological difference in the early vegetative growth of these three classes of plants, it is worthwhile to use molecular biological methods to attempt to identify the sex of a plant at this early growth period. In this s.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.8
- DOI:
- 10.4238/2013.September.27.8
Molecular diversity analysis of eggplant (Solanum melongena) genetic resources
Authors: Z. Ali, Z.L. Xu, D.Y. Zhang, X.L. He, S. Bahadur and J.X. Yi
Eggplant (Solanum melongena), a vegetable that is cultivated worldwide, is of considerable importance to agriculture in China. We analyzed the diversity of this plant using inter-simple sequence repeat (ISSR) and RAPD procedures to subdivide 143 Chinese-cultivated eggplants based on coefficient of parentage, genetic diversity .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1279
- DOI:
- 10.4238/vol10-2gmr1279
Elucidation of thermotolerance diversity in cotton (Gossypium hirsutum L.) using physio-molecular approaches
Authors: R.M. Rana, S.H. Khan, Z. Ali, A.I. Khan and I.A. Khan
Cotton (Gossypium hirsutum) is an important cash crop, but high temperature during its growing season is one of the major factors that limit its productivity. This problem compels plant breeders to breed for heat tolerance, which can help to overcome this challenge. It is very important to make a comprehensive screening of hea.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1180
- DOI:
- 10.4238/vol10-2gmr1180