Rapd
Utilization of molecular markers for the conservation of blood cockles, Anadara granosa (Arcidae)
Authors: S.Y. Chee , M.N.S. Azizah and M.N. Devakie
We examined genetic variation in blood cockles in an effort to obtain information useful for the sustainability, management, and the stability of this species as a major commodity in the fisheries sector. Ten populations of cockles were sampled from the north to the south of the west coast of peninsular Malaysia. The cockles w.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1103
- DOI:
- 10.4238/vol10-2gmr1103
A novel and efficient strategy for practical identification of tomato (Solanum lycopersicon) varieties using modified RAPD fingerprints
Authors: N.K. Korir, X.Y. Li, X.P. Leng, Z. Wu, C. Wang and J.G. Fang
Tomato breeding and variety development have led to the generation of a large number of varieties in many countries worldwide. This has created a growing and urgent need for an improved strategy for genotyping and identification since the traditional methods based on phenotype are growing unreliable. DNA markers could provide distinct benefits in tomato v.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.11.2
- DOI:
- 10.4238/2013.June.11.2
Confirmation of cross-fertilization using molecular markers in ornamental passion flower hybrids
Authors: L.D.H.C.S. Concei�§�£o, G.O. Belo, M.M. Souza, S.F. Santos, C.B.M. Cerqueira-Silva and R.X. Corr�ªa
Several interspecific Passiflora hybrids are produced in the northern hemisphere for the ornamental plant market. In Brazil, production of passion flower hybrids is limited to the introgression of genes into the main cultivated species, yellow passion fruit, to be used as rootstocks. Confirmation of hybridization in the initia.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr894
- DOI:
- 10.4238/vol10-1gmr894
Genetic variation of Casuarina equisetifolia subsp equisetifolia and C. equisetifolia subsp incana populations on the northern coast of Senegal
Authors: A.L. Ndoye, O. Sadio and D. Diouf
The genetic variation of 70 individual samples of Casuarina equisetifolia (L. Johnson) subsp equisetifolia and C. equisetifolia subsp incana growing along the northern coast of Senegal was analyzed with RAPD markers. Of the 160 primers tested, five were chosen; they generated 1396 reproducible bands and 61 polymorphic bands th.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr986
- DOI:
- 10.4238/vol10-1gmr986
Genetic diversity and differentiation in Dalbergia sissoo (Fabaceae) as revealed by RAPD
Authors: B.-Y. Wang, L. Shi, Z.-Y. Ruan and J. Deng
Dalbergia sissoo, a wind-dispersed tropical tree, is one of the most preferred timber tree species of South Asia. Genetic diversity and differentiation among natural populations of D. sissoo were examined for the first time. We found a relatively high level of genetic diversity in D. sissoo, both at the species level (percenta.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr995
- DOI:
- 10.4238/vol10-1gmr995
Identification of sunflower (Helianthus annuus, Asteraceae) hybrids using simple-sequence repeat markers
Authors: A. Iqbal, H.A. Sadaqat, A.S. Khan and M. Amjad
Hybrid identification of 16 sunflower hybrids was confirmed using simple-sequence repeat methodology. Of 20 specific simple-sequence repeat primers, 18 authenticated the purity of these hybrids; the remaining two specific primer pairs gave ambiguous DNA fragments. The results indicate that simple-sequence repeat analysis for t.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr918
- DOI:
- 10.4238/vol10-1gmr918
Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil
Authors: Q.L. Barcelos, E.A. Souza and K.J. Damasceno e Silva
The causal agent of common bean anthracnose, Colletotrichum lindemuthianum, has considerable genetic and pathogenic variability, which makes the development of resistant cultivars difficult. We examined variability within and between Brazilian pathotypes of C. lindemuthianum through the identification of vegetative compatibili.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr907
- DOI:
- 10.4238/vol10-1gmr907
Isolation of genomic DNA from defatted oil seed residue of rapeseed (Brassica napus)
Authors: M. Sadia, M.A. Rabban, S. Hameed, S.R. Pearce and S.A. Malik
A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil-free seed residue of Brassica napus, an important oil seed plant, has been developed. Oil from the dry seeds was completely recovered in an organic solvent and quantified gravimetrically followed by processing of the residual bioma.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1009
- DOI:
- 10.4238/vol10-1gmr1009
Endophytic and pathogenic isolates of the cacao fungal pathogen Moniliophthora perniciosa (Tricholomataceae) are indistinguishable based on genetic and physiological analysis
Authors: T.G. Lana, J.L. Azevedo, A.W.V. Pomella, R.T.R. Monteiro, C.B. Silva and W.L. Ara�ºjo
We evaluated the genetic and physiological variability of Moniliophthora perniciosa obtained from healthy and diseased branches of cacao (Theobroma cacao) plants. The diversity of the isolates was evaluated by RAPD technique and by studies of virulence and exoenzyme production. The genetic variability of endophytic and pathogenic M. perniciosa was evaluat.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr895
- DOI:
- 10.4238/vol10-1gmr895
A new strategy for identification of currant (Ribes nigrum L.) cultivars using RAPD markers
Authors: J. Huo, G. Yang, Y. Zhang and F. Li
We developed a new approach using RAPD fingerprints to distinguish 37 currant cultivars from northeastern China based on optimization of RAPD by choosing 11 nucleotide primers and strict screening PCR annealing temperature. We found that the manual cultivar identification diagram (MCID) approach clearly developed fingerprints from 8 different primers that.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.21.1
- DOI:
- 10.4238/2013.June.21.1