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Rapd

Medical Genetics   Research Article

Evaluation of genetic diversity in Chinese kale (Brassica oleracea L. var. alboglabra Bailey) by using rapid amplified polymorphic DNA and sequence-related amplified polymorphism markers

Authors: J. Zhang and L.G. Zhang1

Chinese kale is an original Chinese vegetable of the Cruciferae family. To select suitable parents for hybrid breeding, we thoroughly analyzed the genetic diversity of Chinese kale. Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) molecular markers were used to evaluate the genetic div.. Read More»

Genet. Mol. Res. 13(2):
2014.February.14.10
DOI:
10.4238/2014.February.14.10
Plant Genetics   Research Article

Genetic relationships of Pacific abalone (Haliotidae) species determined using universal rice primer-polymerase chain reaction fingerprinting

Authors: H.S. An, J.Y. Park, J.I. Myeong and C.M. An

Random amplified polymorphic DNA (RAPD) with universal rice primers (URP) was used to identify species and to determine phylogenetic relationships for the 6 economically important Korean Pacific abalone species: Haliotis discus hannai, H. discus discus, H. madaka, H. gigantea, H. diversicolor supertexta, and H. diversicolor diversicolor, whose morphologic.. Read More»

Genet. Mol. Res. 12(4):
2013.December.4.18
DOI:
10.4238/2013.December.4.18
Plant Genetics   Research Article

Genetic characterization of fig tree mutants with molecular markers

Authors: M.G.F. Rodrigues, A.B.G. Martins, J.A. Desid�©rio, B.W. Bertoni and M.C. Alves

The fig (Ficus carica L.) is a fruit tree of great world importance and, therefore, the genetic improvement becomes an important field of research for better crops, being necessary to gather information on this species, mainly regarding its genetic variability so that appropriate propagation projects and management are made. T.. Read More»

Genet. Mol. Res. 11(3):
2012.August.6.3
DOI:
10.4238/2012.August.6.3
Microbial Genetics   Research Article

A new strategy employed for identification of sweet orange cultivars with RAPD markers

Authors: X. Sun, Q. Mu, D. Jiang , C. Wang, X.C. Wang and J.G. Fang

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency.. Read More»

Genet. Mol. Res. 11(3):
2012.August.6.11
DOI:
10.4238/2012.August.6.11
Microbial Genetics   Research Article

Morphological traits and RAPD markers for characterization and identification of minor Spanish olive cultivars from the Extremadura region

Authors: M.C. Parra-Lobato, F.J. Delgado-Martinez and M.C. Gomez-Jimenez

A total of 91 wild olive accessions and 31 olive cultivars growing in the Extremadura region of central-western Spain were analyzed using morphological traits and RAPD markers. We focused on three main and 16 minor Spanish olive cultivars that are recognized as native or local to the Extremadura region. The five arbitrary 10-mer primers tested on the oliv.. Read More»

Genet. Mol. Res. 11(3):
2012.May.10.6
DOI:
10.4238/2012.May.10.6
Human Genetics   Research Article

Genetic characterization of native and invasive Plagioscion squamosissimus (Perciformes, Sciaenidae) populations in Brazilian hydrographic basins

Authors: R.S. Panarari-Antunes1, A.J. Prioli2, S.M.A.P. Prioli2,3, H.F. J�ºlio Jr2, A.V. Oliveira2,3, C.S. Agostinho4, J.P. Silva Filho5 and L.M. Prioli2

The genetic diversity of invasive and native populations of Plagioscion squamosissimus (Heckel, 1840) from the Paraná, Parnaiba and Araguaia-Tocantins river basins was assessed by using Random Amplified Polymorphic DNA (RAPD) markers. Genetic data confirmed the hypothesis of low genetic variability within and among P. squamosissimus populations int.. Read More»

Genet. Mol. Res. 14(4):
2015.November.13.16
DOI:
10.4238/2015.November.13.16
Plant Genetics   Research Article

An improved strategy based on RAPD markers efficiently identified 95 peach cultivars

Authors: M.L. Yu, W.Y. Wang, R.J. Ma, Z.J. Shen and J.G. Fang

DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time- and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11.. Read More»

Genet. Mol. Res. 11(2):
2012.May.7.1
DOI:
10.4238/2012.May.7.1
Human Genetics   Research Article

RAPD analysis of genetic diversity and population structure of Elymus sibiricus (Poaceae) native to the southeastern Qinghai-Tibet Plateau, China

Authors: X. Ma S.-Y. Chen S.-Q. Bai X.-Q. Zhang D.-X. Li C.-B. Zhang J.-J. Yan

Genetic diversity of Elymus sibiricus (Poaceae) was examined in eight populations from the southeast Qinghai-Tibet Plateau. We detected 291 RAPD polymorphic loci in 93 samples. The percentage of polymorphic bands (PPB) was 79%. Genetic diversity (HE) was 0.264, effective number of alleles (NE) was 1.444, Shannon’s information index (HO) was 0.398, a.. Read More»

Genet. Mol. Res. 11(3):
2012.June.27.5
DOI:
10.4238/2012.June.27.5
Plant Genetics   Research Article

Molecular marker-based genetic diversity analysis of scantly studied Brazilian accessions of a medicinal plant, Morinda citrifolia L. (noni)

Authors: P.N. Bordallo, A.M.R. Monteiro, J.A. Sousa and F.A.S. Arag�£o

Morinda citrifolia L., commonly known as noni, has been used for the treatment of various diseases for over two centuries. It was introduced and widely disseminated in Brazil because of its high market value and ease of adaptation to the soil and climatic conditions of the country. The aim of this study was to estimate the gen.. Read More»

Genet. Mol. Res. 16(1):
gmr16019531
DOI:
10.4238/gmr16019531
Plant Genetics   Research Article

Inter- and intra-specific genetic diversity of Iranian yarrow species Achillea santolina and Achillea tenuifolia based on ISSR and RAPD markers

Authors: M. Ebrahimi M. Farajpour M. Rahimmalek

Cultivation and preservation of yarrow has recently attracted wide attention due to its beneficial properties; however, genetic variation of Achillea species is still relatively unknown. We used RAPD and ISSR markers to assess genetic diversity in 16 accessions of yarrow belonging to two species native to Iran. Seven ISSR and nine RAPD primers generated 1.. Read More»

Genet. Mol. Res. 11(3):
2012.August.27.1
DOI:
10.4238/2012.August.27.1