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Rapd

Plant Genetics   Research Article

SCAR markers for discriminating species of two genera of medicinal plants, Liriope and Ophiopogon

Authors: G. Li Y.-J. Park

The development of DNA markers that can closely discriminate between Liriope and Ophiopogon species is vital for efficient and accurate identification of these species, and to ensure the quality, safety, and efficacy of medicines made from these plants. We developed species-specific molecular markers for these two genera. Forty RAPD primers were tested to de.. Read More»

Genet. Mol. Res. 11(3):
2012.May.18.14
DOI:
10.4238/2012.May.18.14
Bioinformatics   Research Article

A novel strategy for identification of 47 pomegranate (Punica granatum) cultivars using RAPD markers

Authors: Y.P. Zhang H.H. Tan S.Y. Cao X.C. Wang G. Yang J.G. Fang

DNA marker can be used for precise plant cultivar identification. However, DNA markers have often not been used effectively for the identification of plant cultivars due to a lack of an effective analysis strategy. We used a novel strategy for effective identification of plant individuals based on a new way of recording DNA fingerprints of the genotyped plan.. Read More»

Genet. Mol. Res. 11(3):
2012.May.30.1
DOI:
10.4238/2012.May.30.1
Bioinformatics   Research Article

An efficient approach to identify Ginkgo biloba cultivars by using random amplified polymorphic DNA markers with a manual cultivar identification diagram strategy

Authors: G.P. Li C.Q. Zhang F.L. Cao

Cultivar identification is a key step to avoid the formation of homonyms and synonyms of Ginkgo biloba. In this study, a new approach based on combinational utilization of polymorphic bands produced from 6 different random amplified polymorphic DNA (RAPD) primers was developed for identifying 42 Ginkgo cultivars, and a manual cultivar identification diagr.. Read More»

Genet. Mol. Res. 12(1):
2013.January.24.10
DOI:
10.4238/2013.January.24.10
Cytogenomics   Research Article

Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers

Authors: Z. Noormohammadi Y. Hasheminejad-Ahangarani Farahani M. Sheidai S. Ghasemzadeh-Baraki O. Alishah

Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F2 progenies. Ten homo-primers and seven hetero-primers of 26 RAPD.. Read More»

Genet. Mol. Res. 12(1):
2013.January.30.12
DOI:
10.4238/2013.January.30.12
Human Genetics   Research Article

Phenotypic and RAPD diversity among 80 germplasm accessions of the medicinal plant isabgol (Plantago ovata, Plantaginaceae)

Authors: N. Singh, R.K. Lal and A.K. Shasany

Plantago ovata, popularly known as isabgol, has great commercial and medicinal importance due to thin rosy white membranous seed husk. Isabgol seeds and husks have emollient, demulcent and laxative properties. We used both biometric and molecular techniques to assess the genetic variability and relatedness of 80 germplasm acce.. Read More»

Genet. Mol. Res. 8(4):
vol8-4gmr583
DOI:
10.4238/vol8-4gmr583
Plant Genetics   Research Article

Comparison of winter and summer canola (Brassica napus) genotypes in Turkey

Authors: E. Maltas H.C. Vural

We examined genetic relationships between canola (Brassica napus) genotypes cultivated in winter and spring in Turkey. Genomic DNA was isolated from the seeds by two modified CTAB protocols: EZ1 nucleic acid isolation method and a commercial kit (Dneasy Plant Mini Kit, Qiagen). Diversity and genetic relationships in the genotypes were analyzed with RAPD m.. Read More»

Genet. Mol. Res. 12(1):
2013.February.8.5
DOI:
10.4238/2013.February.8.5
Plant Genetics   Research Article

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

Authors: J. Kumchai Y.-C. Wei C.-Y. Lee F.-C. Chen S.-W. Chin

Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them.. Read More»

Genet. Mol. Res. 12(1):
2013.March.13.4
DOI:
10.4238/2013.March.13.4
Plant Genetics   Research Article

Analysis of allopolyploidy-induced rapid genetic and epigenetic changes and their relationship in wheat

Authors: T. Qiu, Y.Z. Dong, X.M. Yu, N. Zhao and Y.F. Yang

We used the conventional and methylation-sensitive randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses to assess genome-wide changes and explore the relationships between genetic and epigenetic variations among individuals of a newly synthesized allohexaploid wheat line whose genomic cons.. Read More»

Genet. Mol. Res. 16(2):
gmr16029303
DOI:
10.4238/gmr16029303
Plant Genetics   Research Article

Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb

Authors: W.H. Zheng, Y. Zhuo, L. Liang, W.Y. Ding, L.Y. Liang and X.F. Wang

Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five.. Read More»

Genet. Mol. Res. 14(3):
http://dx.doi.org/2015.September.8.3
DOI:
http://dx.doi.org/10.4238/2015.September.8.3
Medical Genetics   Research Article

Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy

Authors: J. Han, W.Y. Wang, X.P. Leng, L. Guo, M.L. Yu, W.B. Jiang and R.J. Ma

One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram .. Read More»

Genet. Mol. Res. 13(1):
2014.January.8.2
DOI:
10.4238/2014.January.8.2