Rapd
RAPD assay of wild-type olives in Turkey
Authors: M. Sesli and E.D. YeÃ?Â?enoÃ?Â?lu
Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers ob.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr783
- DOI:
- 10.4238/vol9-2gmr783
Genetic variability of Mahanarva sp (Hemiptera: Cercopidae) collected from different sites in Brazil
Authors: A.M. Auad, R. Domingues, M.A. Machado, L.S. Souza, G.S. Carvalho and S.V. Paula-Moraes
Spittlebugs are the leading cause of damage to tall grasses. Annual losses are estimated to reach 2.1 billion dollars in sugarcane crops and grazing land throughout the world. Correct identification of these species is difficult due to similarities in color, body size and male genitalia. Molecular markers have been useful in t.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr794
- DOI:
- 10.4238/vol9-2gmr794
Genetic relationships among turnip (Brassica rapa var. rapa) genotypes
Authors: E. Yildirim, N. Yildirim, S. Ercisli, G. Agar and H. Karlidag
Turnip (Brassica rapa var. rapa) is one of the main vegetables consumed by people living in Eastern Anatolia in Turkey. In this region, farmers obtain their own seeds for production, which results in considerable morphological variability. We examined the genetic variation and relationships among 11 turnip genotypes sampled fr.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr786
- DOI:
- 10.4238/vol9-2gmr786
Conservation implications of the genetic diversity of Gymnospermium microrrhynchum in Korea
Authors: S.H. Lee, M.H. Yeon and J.K. Shim
Gymnospermium microrrhynchum (Berberidaceae) is an ephemeral perennial herb with a limited distributional range in the Baekdudaegan mountain areas of the Korean Peninsula, and is designated a rare plant by the Korean Forest Service. Information about its genetic variation and structure is important for developing successful co.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048843
- DOI:
- 10.4238/gmr15048843
Genetic characterization and authentication of Dimocarpus longan Lour. using an improved RAPD technique
Authors: Z.Q. Mei, S.Y. Fu, H.Q. Yu1, L.Q. Yang, C.G. Duan, X.Y. Liu, S. Gong1 and J.J. Fu
Dimocarpus longan Lour. is an edible and traditional herb in China, commonly referred to as longon. An improved randomly amplified polymorphic DNA (RAPD) protocol was here developed in order to determine the geographical origins of D. longan samples collected from 5 provinces in the southern and southwestern areas of China, in.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.6.3
- DOI:
- http://dx.doi.org/10.4238/2014.March.6.3
Fine-scale genetic structure patterns in two freshwater fish species, Geophagus brasiliensis (Osteichthyes, Cichlidae) and Astyanax altiparanae (Osteichthyes, Characidae) throughout a Neotropical stream
Authors: D.G. Ferreira1, S.C. Lima3, W. Frantine-Silva3, J.F. Silva1, C. Apolin�¡rio-Silva3, S.H. Sofia3, S. Carvalho2 and B.A. Galindo1
Streams are very important environments for Neotropical freshwater fish fauna, and possess a high number of species. These small drainages are also highlighted by their intrinsic biological and physicochemical features; however, knowledge on the genetic distribution of fish in these drainages is limited. Therefore, in the pres.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048124
- DOI:
- 10.4238/gmr15048124
RAPD and ISSR markers in the evaluation of genetic divergence among accessions of elephant grass
Authors: R.S.N de Lima, R.F. Daher, L.S.A. Gon�§alves, D.A. Rossi, A.T. do Amaral J�ºnior, M.G. Pereira and F.J.S. L�©do
Considering the expected genetic variability of elephant grass (Pennisetum purpureum), due to its cultivation in different continents, we characterized and estimated the genetic divergences between 46 accessions of elephant grass with different edaphoclimatic adaptations, using RAPD and ISSR markers. We evaluated, comparativel.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1107
- DOI:
- 10.4238/vol10-3gmr1107
Efficacy of random primer-pair arrays in plant genome analysis: a case study of Cucumis (Cucurbitaceae) for identification of wild and cultivated species
Authors: E.M. Gatphoh, S.K. Sharma, K. Rajkumari and S. Rama Rao
The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, bo.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1083
- DOI:
- 10.4238/vol10-3gmr1083
Isolation and characterization of microsatellite loci in the fish Coilia mystus (Clupeiformes: Engraulidae) using PCR-based isolation of microsatellite arrays
Authors: J.-Q. Yang, X.-D. Zhou, D. Liu, Z.-Z. Liu and W.-Q. Tang
Coilia mystus is the most important harvested fish species in China; it inhabits quite different water environments during the different life history stages. Populations of C. mystus have dropped sharply due to overharvesting and water pollution. We developed eight microsatellite loci in C. mystus for conservation genetics stu.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1420
- DOI:
- 10.4238/vol10-3gmr1420
The use of RAPD to characterize Bipolaris sorokiniana isolates
Authors: Maria Viviane Gomes M�¼ller, Jos�© Carlos Germani and Sueli Teresinha Van Der Sand
Bipolaris sorokiniana is a phytopathogenic fungus causing diseases of cereal crops such as common root rot, the leaf spot disease, seedling blight, and black point of the grain. Random-amplified polymorphic DNA (RAPD) assay was used to investigate the genetic diversity of 20 isolates collected from different cultivars in wheat.. Read More»
- Genet. Mol. Res. 4(4):