Rapd
Applicability of RAPD markers on silverstained polyacrylamide gels to ascertain genetic diversity in Peripatus acacioi (Peripatidae; Onychophora)
Authors: Daiane Mariele DeLaat, Maria Raquel Santos Carvalho, Maria Bernadete Lovato, Maria Dolores Porto Acedo and Cleusa Gra�§a da Fonseca
RAPD (random amplification of polymorphic DNA) molecular markers can be utilized for analyzing genetic variability in populations for which only a few or no molecular markers are available. They were used in a study of an endangered species, Peripatus acacioi, found in the Tripuí Ecological Station, in Ouro Preto, MG, B.. Read More»
- Genet. Mol. Res. 4(4):
Molecular characterization of urdbean (Vigna mungo) germplasm related to resistance against urdbean leaf crinkle virus
Authors: R. Binyamin, M. Aslam Khan, A.I. Khan, M. Azam Khan, F.S. Awan and N.A. Khan
Urdbean (Vigna mungo) is an important pulse crop grown worldwide. Urdbean leaf crinkle virus (ULCV) is a pathogen of urdbean found in Pakistan that causes huge losses in yield. Forty urdbean varieties/lines were screened against the virus under field conditions during spring season 2009. None of the lines appeared to be highly.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1446
- DOI:
- 10.4238/vol10-3gmr1446
A protocol for high-quality genomic DNA extraction from legumes
Authors: I.O. Agbagwa S. Datta P.G. Patil P. Singh N. Nadarajan
Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding li.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.14.1
- DOI:
- 10.4238/2012.September.14.1
RAPD and ISSR-assisted identification and development of three new SCAR markers specific for the Thinopyrum elongatum E (Poaceae) genome
Authors: G.H. Xu, W.Y. Su, Y.J. Shu, W.W. Cong, L. Wu and C.H. Guo
Diploid Thinopyrum elongatum, a wild relative of wheat, contains many agronomically desirable traits and has potential for increasing genetic variability and introducing desirable characters in this crop. Few molecular markers are available for rapid screening of T. elongatum genome segments in the wheat genetic background. We.. Read More»
- Genet. Mol. Res. 11(2):
- http://dx.doi.org/2012.June.29.7
- DOI:
- http://dx.doi.org/10.4238/2012.June.29.7
Food assimilated by two sympatric populations of the brown planthopper Nilaparvata lugens (Delphacidae) feeding on different host plants contaminates insect DNA detected by RAPD-PCR analysis
Authors: M.A. Latif, M.Y. Omar, S.G. Tan, S.S. Siraj, M.E. Ali and M.Y. Rafii
Contamination of insect DNA for RAPD-PCR analysis can be a problem because many primers are non-specific and DNA from parasites or gut contents may be simultaneously extracted along with that of the insect. We measured the quantity of food ingested and assimilated by two sympatric populations of brown planthopper (BPH), Nilapa.. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.9.4
- DOI:
- http://dx.doi.org/10.4238/2012.January.9.4
Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR
Authors: M.T.H. Shahid, F.A. Khan, A. Saeed and I. Fareed
Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis a.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1122
- DOI:
- 10.4238/vol10-3gmr1122
Schistosome/mollusk: genetic compatibility
Authors: A.L.D. Oliveira, D. Da Silva, E.M. Zanotti-Magalhaes,A.Z. Abdel-Hamid, J.T. Ribeiro-Paes
Schistosomiasis remains one of the most prevalent parasitic infections and has significant economic and public health consequences in many developing countries. Economic development and improvement in standard of living in these countries are dependent on the elimination of this odious disease. For the control of Schistosomias.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr444
- DOI:
- 10.4238/vol7-2gmr444
Comparative genetic analysis of trichome-less and normal pod genotypes of Mucuna pruriens (Fabaceae)
Authors: S.S. Dhawan, G.K. Rai, M.P. Darokar, R.K. Lal, H.O. Misra and S.P.S. Khanuja
Velvet bean (Mucuna pruriens) seeds contain the catecholic amino acid L-DoPA (L-3,4-dihydroxyphenylalanine), which is a neurotransmitter precursor and used for the treatment of Parkinson’s disease and mental disorders. The great demand for L-DoPA is largely met by the pharmaceutical industry through extraction of the com.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1264
- DOI:
- 10.4238/vol10-3gmr1264
Genetic and biochemical differentiation in Vitis vinifera (Kabarcik) populations grown at different altitudes in Coruh Valley
Authors: G. Agar, N. Yildirim and S. Ercisli
We examined genetic differences of four Vitis vinifera populations (A, B, C, D) including local Kabarcik cultivar grown along an altitude gradient of 800, 900, 1000, and 1150 m above sea level in the Coruh Valley (800 m: A population; 900 m: B population; 1000 m: C population; 1150 m: D population). Leaf samples were used for .. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.February.3.1
- DOI:
- http://dx.doi.org/10.4238/2012.February.3.1
Morphometric and molecular analysis of mackerel (Rastrelliger spp) from the west coast of Peninsular Malaysia
Authors: M.N. Darlina, A.R. Masazurah, P. Jayasankar, A.F.J. Jamsari and A.M.N. Siti
Mackerel (Scombridae; Rastrelliger) are small commercially important pelagic fish found in tropical regions. They serve as a cheap source of animal protein and are commonly used as live bait. By using a truss morphometrics protocol and RAPD analysis, we examined morphological and genetic variation among 77 individual mackerel .. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1249
- DOI:
- 10.4238/vol10-3gmr1249