Regeneration
Investigation on possible occurrence of somaclonal effects in zygotic and embryonic cotton structures based on agronomical traits
Authors: T.C. Soares, J.M.F.C. Carvalho, L.M. Lima, R.M.M. Freire,C.R.C. Silva, P.A. Melo Filho and R.C. Santos
Zygotic and embryonic cotton structures were used in in vitro procedures to obtain stable plants. Zygotes (24, 48, and 72 h) and immature embryos (10 and 25 days after fertilization) were grown in MS medium supplemented with glucose, indoleacetic acid, kinetin, and activated charcoal. Regenerated seedlings were acclimatized an.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039711
- DOI:
- 10.4238/gmr16039711
Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus
Authors: L.N. Sun1,2, H.S. Yang1, M.Y. Chen3 and D.X. Xu1,2
Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein con.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.7
- DOI:
- 10.4238/2013.November.7.7
Role of death receptors in the regulation of hepatocyte proliferation and apoptosis during rat liver regeneration
Authors: Y. Zhou1,2, J.C. Xu2, Y.F. Jia1 and C.S. Xu1
The balance between hepatocyte proliferation and apoptosis is critical for liver homeostasis during liver regeneration. We created a rat liver regeneration model by partial hepatectomy (PH) to investigate the overall mechanism that regulates the proliferation and apoptosis of hepatocytes. The Rat Genome 230 2.0 Array was used to investigate changes in the.. Read More»
- Genet. Mol. Res. 14(4):
- 0.4238/2015.October.29.26
- DOI:
- 0.4238/2015.October.29.26
miR-71b regulation of insulin/IGF-1 signaling during starvation in planarians
Authors: Y.Y. Wu, J.M. Zhao, Q. Liu, Q. Guo, Z. Liu, X.X. Wang, C.Y. Wang, R.Y. Li, Y.Z. Zhang and S.T. Zhang
Planarians, which have a large population of stem cells called neoblasts, are molecularly tractable model systems used in the study of regeneration. However, planarians have strong resistance to hunger and have developed growth arrest strategies. For example, they can change their size and undergo growth regression during star.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.4
- DOI:
- 10.4238/2015.October.5.4
In vitro regeneration of cocona (Solanum sessiliflorum, Solanaceae) cultivars for commercial production
Authors: A.R. Schuelter1, A.K. Grunvald1, A.T. Amaral J�ºnior2, C.L. da Luz1,C.L. Luz1, L.M. Gon�§alves1, S. Stefanello1 and C.A. Scapim3
Cocona (Solanum sessiliflorum Dunal) is a solanaceous shrub native to the Amazon region that produces an edible fruit. This species has numerous advantages, particularly a high nutritional value and productivity. However, due to irregular germination and rapid loss of seed viability, there are few plantations for production on a large scale. Development o.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr613
- DOI:
- 10.4238/vol8-3gmr613
Cloning and prokaryotic expression of rat homolog of Serpina3n and its expression change during liver regeneration
Authors: G.P. Wang X.S. Zhang Y.H. Li J.L. Zheng C.Z. Tang W.X. Zhang
A strikingly upregulated expressed sequence tag was screened from regenerating rat liver at 8 h in a 0-4-8-12 h short-interval successive partial hepatectomy model from a previous study. In the present study, a full-length open reading frame (ORF) corresponding to this expressed sequence tag was predicted through electronic cloning and was subsequently cl.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.3.6
- DOI:
- 10.4238/2012.September.3.6
Expression profiles uncover the relevance between colony stimulating factor-mediated signaling pathways in liver cells and partial hepatectomy-induced hepatic regeneration
Authors: X.G. Chen and C.S. Xu
Colony stimulating factors (CSF) have been considered to modulate liver regeneration (LR) after partial hepatectomy (PH) at the tissue level. However, it remains unclear about precise mechanism of action of CSF in regeneration at the cellular level. Therefore, eight rat liver cell types were isolated by Percoll gradient centri.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.February.14.17
- DOI:
- 10.4238/2014.February.14.17
Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean
Authors: J. Sun, J. Li, M. Liu, B.B. Zhang, D.M. Li, M. Wang, C. Zhang,W.B. Li, A.Y. Su and X.X. Wu
The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA librar.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.20
- DOI:
- 10.4238/2015.January.30.20
Expression profiles of genes associated with mitochondria-mediated apoptosis and their roles in liver regeneration
Authors: X.K. Xing, M.H. Li, Z.W. Guo and C.S. Xu
Mitochondria are closely associated with cell survival, and it is of interest to determine whether apoptosis pathways, which are mediated by mitochondria, are involved in liver regeneration (LR). To identify the mechanisms underlying mitochondria-mediated apoptosis during rat LR, we used the Rat Genome 230 2.0 Array to investigate changes in gene expressi.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038101
- DOI:
- 10.4238/gmr.15038101
Agrobacterium-mediated transformation of tomato (Solanum lycopersicum L.) using the expansin 10 (CsEXP10) gene
Authors: Y.D. Sun, W.R. Luo, S.Y. Sun and L. Ni
The cucumber expansin 10 (CsEXP10) gene was previously cloned from young cucumber fruits but its role has not been defined. To determine the role of this gene in plant growth and development, a CsEXP10 gene transformation system was established. The open reading frame of the gene was inserted behind the CaMV35S promoter of vector pCAMBIA1301, and the cons.. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015