Rna
Assessing potential miRNA targets based on a Markov model
Authors: Hao-Yue Fu1,2, Ding-Yu Xue2, Xiang-de Zhang1 and Pei-Ying Yang2
At present, studies on microRNA mainly focus on the identification of microRNA genes and their mRNA targets. Although researchers have identified many microRNA genes, relatively few microRNA targets have been identified by experimental methods. Computational programs designed for predicting potential microRNA targets provide numerous targets for expe.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr604
- DOI:
- 10.4238/vol8-3gmr604
MicroRNA-139 modulates Alzheimerâ??s-associated pathogenesis in SAMP8 mice by targeting cannabinoid receptor type 2
Authors: Y. Tang, J.S. Bao, J.H. Su and W. Huang
Alzheimer’s disease (AD) is a neurodegenerative disorder, and is the most common type of dementia in the elderly population. Growing evidence indicates that microRNAs (miRNAs) play a crucial role in neuroinflammation associated with AD progression. In this study, we analyzed the expression of microRNA-139 (miR-139) as we.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019166
- DOI:
- 10.4238/gmr16019166
Bottom-up assembly of RNA nanoparticles containing phi29 motor pRNA to silence the asthma STAT5b gene
Authors: C. Qiu W.K. Peng F. Shi T. Zhang
Activation of the transcription factor signal transducer and activator of transcription 5b (STAT5b) is a key event in the development of asthma. The potent ability of small interfering RNA (siRNA) to inhibit the expression of STAT5b mRNA has provided a new class of therapeutics for asthma. However, efficient delivery of siRNAs remains a key obstacle to th.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.12.6
- DOI:
- 10.4238/2012.September.12.6
Alpaca fiber growth is mediated by microRNA let-7b via down-regulation of target gene FGF5
Authors: T. Wang, Y. Zhang, H.D. Wang, Y. Shen, N. Liu, J. Cao, X.J. Yu, C.S. Dong and X.Y. He
MicroRNAs are very small endogenous RNA molecules that play a crucial role in an array of biological processes, including regulation of skin morphogenesis. The microRNA let-7b is thought to modulate animal hair growth, by binding target genes that encode growth factors. Fibroblast growth factor 5 (FGF5) has been previously reported to be involved in the i.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.38
- DOI:
- 10.4238/2015.October.28.38
Supplemental leucine and isoleucine affect expression of cationic amino acid transporters and myosin, serum concentration of amino acids, and growth performance of pigs
Authors: M. Cervantes-Ram?rez V. Mendez-Trujillo B.A. Araiza-Pi?±a M.A. Barrera-Silva D. Gonz?¡lez-Mendoza A. Morales-Trejo
Leucine (Leu) participates in the activity of cationic amino acid (aa) transporters. Also, branched-chain aa [Leu, isoleucine (Ile), and valine (Val)] share intestinal transporters for absorption. We conducted an experiment with 16 young pigs (body weight of about 16 kg) to determine whether Leu and Ile affect expression of aa transporters b0,+ and CAT-1 .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.3
- DOI:
- 10.4238/2013.January.24.3
Abnormal metabolites related to bone marrow failure in aplastic anemia patients
Authors: P. Zhong, J. Zhang and X. Cui
Metabolomics is the identification and quantitation of small molecules that are involved in metabolic reactions. Liquid chromatography-mass spectrometry (LC-MS) has enjoyed growing popularity as the platform for metabolomic studies due to high throughput, soft ionization, and good coverage of metabolites. Serum metabolites of aplastic anemia (AA) patients.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.33
- DOI:
- 10.4238/2015.October.28.33
Efficient isolation of high-quality RNA from lotus Nelumbo nucifera ssp nucifera tissues
Authors: Y.J. Zhang X.Y. Hao Z.S. Liang W.D. Ke H.B. Guo
Nelumbo nucifera is widely used as food, as an ornamental, in medicine, and as packing material; it is also reported to have anti-HIV effects and antioxidant capacity. We sought an improved method for extracting high-quality total RNA from different tissues of N. nucifera. Four methods for RNA extraction were assessed for their ability to recover high-qua.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.14
- DOI:
- 10.4238/2013.January.24.14
Internal phloem in an interspecific hybrid of cassava, an indicator of breeding value for drought resistance
Authors: D. Graciano-Ribeiro, D.Y.C. Hashimoto, L.C. Nogueira, D. Teodoro, S.F. Miranda and N.M.A. Nassar
We examined the stem anatomy of Manihot esculenta Crantz and its hybrid with M. oligantha Pax. Cross-sections were stained with safranin-alcian blue. Manihot esculenta and its hybrid were found to have the same vascular structure in bicollateral bundles with internal phloem, which has been associated with drought resistance in.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr629
- DOI:
- 10.4238/vol8-3gmr629
Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells
Authors: B. Canto-Canch?© M. Tzec-Sim?¡ J.I. V?¡zquez-Lor?a H. Espadas-?lvarez B.H. Ch?-Manzanero R. Rojas-Herrera R. Valdez-Ojeda L. Alzate-Gaviria
Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) - a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the mi.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.4.2
- DOI:
- 10.4238/2013.February.4.2
An alternate method for DNA and RNA extraction from clotted blood
Authors: Z. Zakaria S.H. Umi S.S. Mokhtar U. Mokhtar M.Z. Zaiharina A.T.A. Aziz B.P. Hoh
We developed an alternative method to extract DNA and RNA from clotted blood for genomic and molecular investigations. A combination of the TRIzol method and the QIAamp spin column were used to extract RNA from frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood Mini Kit was used for DNA extraction. Extracted DNA and RNA were a.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.4.4
- DOI:
- 10.4238/2013.February.4.4