Rna
Effects of exogenous protease supplementation on endogenous trypsin activity and gene expression in broilers
Authors: L. Yuan, S.Q. Wang, Z.X. Wang, H. Zhu and K. Huang
Two hundred and forty one-day-old male broiler chickens (Ross-308) were fed corn-soybean basal diets with 0, 40, 80, or 160 mg/kg of an acid protease preparation from 1 to 42 days of age, in order to investigate the effects of an exogenous enzyme on growth performance, pancreatic trypsin activity and mRNA expression. Average daily gain (ADG) was greatest .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.25
- DOI:
- 10.4238/2015.October.28.25
Characterization of eight novel microsatellite markers in the green-lipped mussel Perna viridis (Mytilidae)
Authors: Y.Y. Cao Z.B. Li Q.H. Li X.J. Chen L. Chen G. Dai
The green lipped mussel, also known as the Asian green mussel (Perna viridis) is a fast reproducing and valuable food source, but it is also considered an invasive species and can clog and damage pipes and marine equipment. Eight novel polymorphic microsatellite loci for P. viridis were isolated and characterized. Microsatellite polymorphism was evaluated.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.7.4
- DOI:
- 10.4238/2013.February.7.4
Comparative evaluation of total RNA extraction methods in Theobroma cacao using shoot apical meristems
Authors: D.V. Silva1, S.M.J. Branco1, I.S.A. Holanda2, S. Royaert3, J.C. Motamayor4,J.P. Marelli4 and R.X. Corr�?ªa5
Theobroma cacao is a species of great economic importance with its beans used for chocolate production. The tree has been a target of various molecular studies. It contains many polyphenols, which complicate the extraction of nucleic acids with the extraction protocols requiring a large amount of plant material. These issues, therefore, necessitate the op.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017364
- DOI:
- 10.4238/gmr.15017364
Differential expression of FUT1 and FUT2 in Large White, Meishan, and Sutai porcine breeds
Authors: R.W. Xia1, W.Y. Qin1, L.N. Gan1, J. Wang1, S.L. Wu1,2 and W.B. Bao1,2
To assess the relationship between the expression of a(1,2)-fucosyltransferase (FUT1 and FUT2) genes and resistance to Escherichia coli F18 in weaned pigs, FUT1 and FUT2 expression levels in Large White, Meishan, and Sutai pigs (with resistance to E. coli F18) were determined using real-time PCR. The results revealed that FUT1 and FUT2 expression levels w.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017613
- DOI:
- 10.4238/gmr.15017613
Molecular characterization, expression, and functional analysis of chicken TRAF6
Authors: J. Jin, J.S. Ran, C.W. Yang, X.S. Jiang, Y.G. Zhou, Z.Q. Feng, Y. Wang, D. Lan, P. Ren and Y.P. Liu
Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial adaptor molecule of the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily, which can trigger downstream signaling cascades involved in innate immunity. The function of TRAF6 has been clarified in mammals but is poorly understood in chicken. .. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019138
- DOI:
- 10.4238/gmr16019138
Expression of microRNA-210 in tissue and serum of renal carcinoma patients and its effect on renal carcinoma cell proliferation, apoptosis, and invasion
Authors: T.Y. Liu1, H. Zhang2, S.M. Du3, J. Li1 and X.H. Wen1
This study investigated the expression of microRNA-210 (miR-210) in tissue and serum of renal carcinoma patients and its effect on renal carcinoma cell proliferation, apoptosis, and invasion. Thirty-two renal carcinoma patients in our hospital were selected as the study group and 32 people receiving a physical examination were selected as the control grou.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017746
- DOI:
- 10.4238/gmr.15017746
Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases
Authors: J. Luo, K. Yang and Y.G. Wen
The incidence of liver cancer has gradually risen to a high level in China, and tumor metastasis occurs via multiple pathways. Alpha fetal protein (AFP) is the main biomarker of liver cancer micrometastases. A recent study showed that glypican-3 (GPC3), which is abundant in hepatoma cells, has promising specificity and could b.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16018947
- DOI:
- 10.4238/gmr16018947
Differential expression analysis of porcine MDH1, MDH2 and ME1 genes in adipose tissues
Authors: S.L. Zhou, M.Z. Li, Q.H. Li, J.Q. Guan and X.W. Li
Malate dehydrogenases 1 and 2 (MDH1 and MDH2), and malic enzyme 1 (ME1) play important roles in the Krebs cycle for energy metabolism. The mRNA abundance changes of MDH1, MDH2 and ME1 genes were measured across six different adipose tissues from the leaner Landrace and fatty Rongchang pig breeds using quantitative real-time PC.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.9.4
- DOI:
- 10.4238/2012.May.9.4
Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes
Authors: S. Wei L.S. Zan H.B. Wang G. Cheng M. Du Z. Jiang G.J. Hausman D.C. McFarland M.V. Dodson
Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine. We designed and selected an effective shRNA (small hairpin RNA) against bovine FABP4, c.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.4.21
- DOI:
- 10.4238/2013.January.4.21
Genome wide association study on early puberty in Bos indicus
Authors: A.V. Nascimento1, M.C. Matos2, L.O. Seno3, A.R.S. Romero1, J.F. Garcia4 andA.B. Grisolia1
The aim of this study was to evaluate a genome wide association study (GWAS) approach to identify single nucleotide polymorphisms (SNPs) associated with fertility traits (early puberty) in Nellore cattle (Bos indicus). Fifty-five Nellore cows were selected from a herd monitored for early puberty onset (positive pregnancy at 18 months of age). Extremes of .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017548
- DOI:
- 10.4238/gmr.15017548