Rna
Integrated microRNA-mRNA analysis of pancreatic ductal adenocarcinoma
Authors: P.F. Liu, W.H. Jiang, Y.T. Han, L.F. He, H.L. Zhang and H. Ren
The main aim of this study was to explore the underlying molecular mechanisms and potential target molecules of pancreatic adenocarcinoma. The miRNA (GSE32678) and mRNA (GSE32676) expression profiles of patients with pancreatic ductal adenocarcinoma and healthy controls were downloaded from the Gene Expression Omnibus database. Differentially expressed mi.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.August.28.14
- DOI:
- http://dx.doi.org/10.4238/2015.August.28.14
Characterization of a novel CAPN3 transcript generated by alternative splicing in cattle
Authors: S.Y. Liu, H. Jiang, B. Yuan, Y. Gao, L.S. Dai and J.B. Zhang
Calpain-3 (CAPN3) is a member of the calpain family of Ca2+-regulated cysteine proteases, which play an important role in sarcomere remodeling and mitochondrial protein turnover, and thus, regulating beef tenderness in cattle. Currently, multiple CAPN3 transcripts have been detected in human, monkey, rat, and rabbit. However, whether this transcript is pr.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.20
- DOI:
- 10.4238/2015.January.23.20
Impact of BMMSCs from different sources on proliferation of CD34+ cells
Authors: R.F. Liu, J.Q. Li, R.X. Hou, R. Wang and K.M. Zhang
There are significant differences on the biological characteristics of bone marrow mesenchymal stem cells (BMMSCs), immunological response, and antigen-presenting functions between patients with psoriasis and normal subjects, but there are no significant differences in aborted fetuses. We examined the differences in BMMSCs between aborted fetuses and patient.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.22
- DOI:
- 10.4238/2015.January.23.22
A rapid and high-quality method for total RNA isolation from Haematococcus pluvialis
Authors: Q.L. Luo, K.P. Wang, K. Xiao, C.G. Wang and Z.L. Hu
Haematococcus pluvialis, as the most potential natural source of astaxanthin, which is a powerful antioxidant with high economic value, has attracted more and more scientific attention in recent years. An in-depth understanding of the mechanism for how H. pluvialis produces astaxanthin requires the intensive investigations on .. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029614
- DOI:
- 10.4238/gmr16029614
Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy
Authors: J. Han, W.Y. Wang, X.P. Leng, L. Guo, M.L. Yu, W.B. Jiang and R.J. Ma
One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.8.2
- DOI:
- 10.4238/2014.January.8.2
Effect of miRNA-203 on cervical cancer cells and its underlying mechanism
Authors: X.Z. Yin, D.M. Zhao, G.X. Zhang and L. Liu
miRNA-203 is involved in the development and progression of various types of cancer. However, its role in cervical cancer remains unclear. The aim of this study was to investigate the effect of miRNA-203 on the proliferation and migration of HeLa cervical cancer cells, as well as survivin expression in these cells. A miRNA-203 primer probe was designed ac.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038680
- DOI:
- 10.4238/gmr.15038680
A rapid and efficient method for isolation of total RNA from Euglena gracilis (Euglenoidea)
Authors: Euglena gracilis; Sodium dodecyl sulfate-Tris;RNA isolation; Reverse transcription-polymerase chain reaction
RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently, there is no commercial kit .. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr590
- DOI:
- 10.4238/vol8-2gmr590
Isolation and characterization of polymorphic microsatellite markers in Bagarius yarrelli using RNA-Seq
Authors: M. Du, Y.H. Liu and B.Z. Niu
The yellow sisorid catfish (Bagarius yarrelli) is a vulnerable fish species. In this study, seven polymorphic microsatellite DNA markers for yellow sisorid catfish were described, using RNA-Seq methodology. In B. yarrelli (N = 44) from a Hekou wild population, allelic frequency, and observed and expected heterozygosities per locus varied from two to six, .. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Cloning and identification of the ASIP gene in Chinese raccoon dog (Nyctereutes procyonoides procyonoides)
Authors: Y.M. Li, S. Si, P.C. Guo, L.L. Li, C.Y. Bai and S.Q. Yan
The quantity, quality, and distribution of eumelanin and pheomelanin determine a wide variety of coat colors in animals. Three coat color variants exist in farmed wild-type Chinese raccoon dog (Nyctereutes procyonoides procyonoides), which is an important fur-bearing animal species. The ASIP gene is an important candidate gene for coat color variation in .. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Diversity of plant growth-promoting bacteria associated with sugarcane
Authors: J.E.L. Antunes, M.C.C.P. Lyra, F.J. Ollero, A.D.S. Freitas, L.M.S. Oliveira, A.S.F. Ara�ºjo and M.V.B. Figueiredo
The sugarcane (Saccharum spp) presents economic importance, mainly for tropical regions, being an important Brazilian commodity. However, this crop is strongly dependent on fertilizers, mainly nitrogen (N). This study assessed the plant growth-promoting bacteria (PGPB) associated with sugarcane that could be used as a potentia.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029662
- DOI:
- 10.4238/gmr16029662