Rna
RNA-seq reveals the downregulated proteins related to photosynthesis in growth-inhibited rice seedlings induced by low-energy N+ beam implantation
Authors: Q.F. Chen, H.Y. Ya, W.D. Wang and Z. Jiao
Transcriptomic research based on RNA sequencing (RNA-seq) technology is innovative and will provide new opportunities and biological perspectives for the use of ion-beam implantation in plants. Using RNA-seq, transcriptomes of whole rice seedlings generated from seeds implanted with a low-energy N+ beam were analyzed 96 h afte.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.March.26.9
- DOI:
- 10.4238/2014.March.26.9
Differential expression of peroxisome proliferator-activated receptor �³, fatty acid synthase, and hormone-sensitive lipase in fat-tailed and thin-tailed sheep breeds
Authors: X.C. Xu,B.B. Li,X. Wei,Y.X. Yang,X.L. Wang,Y.L. Chen
Tail fat content affects meat quality, and it varies in different sheep breeds. Theoretically, lipid metabolism contributes to variation in tail fat content. Tail length, tail width, and tail girth were measured in live Tong sheep (with both short fat tail and long fat tail), Shaanbei fine wool sheep (long thin tail), Tan shee.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.14
- DOI:
- 10.4238/2015.December.1.14
Computational identification of miRNAs and their targets in Phaseolus vulgaris
Authors: J. Han, H. Xie, M.L. Kong, Q.P. Sun, R.Z. Li and J.B. Pan
MicroRNAs (miRNAs) are a class of non-coding small RNAs that negatively regulate gene expression at the post-transcriptional level. Although thousands of miRNAs have been identified in plants, limited information is available about miRNAs in Phaseolus vulgaris, despite it being an important food legume worldwide. The high cons.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.17.16
- DOI:
- 10.4238/2014.January.17.16
Matrix metalloproteinase-3 gene polymorphism and its mRNA expression in rheumatoid arthritis
Authors: M.J. Ma, H.C. Liu, X.Q. Qu, J.L. Wang
Matrix metalloproteinase-3 (MMP-3) can mediate the occurrence and development of rheumatoid arthritis (RA). The MMP3 promoter gene exhibits polymorphism with 5A/6A alleles. We investigated the correlation between the expression of MMP3 gene polymorphism and RA to provide an objective basis for prognosis evaluation. We enrolled.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.17
- DOI:
- 10.4238/2015.December.1.17
Genetic variability in Melipona quinquefasciata (Hymenoptera, Apidae, Meliponini) from northeastern Brazil determined using the first internal transcribed spacer (ITS1)
Authors: J.O.P. Pereira, B.M. Freitas, D.M.M. Jorge, D.C. Torres,C.E.A. Soares and T.B. Grangeiro
Melipona quinquefasciata is a ground-nesting South American stingless bee whose geographic distribution was believed to comprise only the central and southern states of Brazil. We obtained partial sequences (about 500-570 bp) of first internal transcribed spacer (ITS1) nuclear ribosomal DNA from Melipona specimens putatively i.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2kerr025
- DOI:
- 10.4238/vol8-2kerr025
Molecular characterization of Anthurium genotypes by using DNA fingerprinting and SPAR markers
Authors: J.D. Souza Neto, T.C.B. Soares, L.B. Motta, P.D.S. Cabral and J.A. Silva
We characterized single primer amplification reaction (SPAR) molecular markers from 20 genotypes of Anthurium andraeanum Lind., including 3 from commercial varieties and 17 from 2 communities in the State of Espírito Santo, Brazil. Twenty-four SPAR, consisting of 7 random amplified polymorphic DNA and 17 inter-.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.2.6
- DOI:
- 10.4238/2014.July.2.6
Multivariate analysis of morphological characteristics of two species of passion flower with ornamental potential and of hybrids between them
Authors: E.A. Santos, M.M. Souza, A.P. Viana, A.A.F. Almeida, J.C.O. Freitas and P.R. Lawinscky
We estimated genetic parameters through multivariate analysis of two species of Passiflora and their hybrids, considered of ornamental potential, based on the morphological characteristics: flower diameter, corona diameter, corona filament size, flower peduncle length, petal length and width, sepal length and width, internode .. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.13.3
- DOI:
- 10.4238/2011.October.13.3
Optimization of PCR conditions to amplify Cyt b, COI and 12S rRNA gene fragments of Malayan gaur (Bos gaurus hubbacki) mtDNA
Authors: M.K.A. Rosli, A.S. Zamzuriada, S.M.F. Syed-Shabthar, M.C. Mahani, O. Abas-Mazni and B.M. Md-Zain
PCR has been extensively used for amplification of DNA sequences. We conducted a study to obtain the best amplification conditions for cytochrome b (Cyt b), cytochrome c oxidase I (COI) and 12S rRNA (12S) gene fragments of Malayan gaur mtDNA. DNA from seven Malayan gaur samples were extracted for PCR amplification. Various tri.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.19.2
- DOI:
- 10.4238/2011.October.19.2
Expression patterns of two heat-shock cognate 70 genes during immune responses and larval development of the Chinese mitten crab Eriocheir sinensis
Authors: P. Li, X.F. Jiang, W.B. Guo, J. Yan and K.Y. Zhou
Two heat-shock protein (HSP) 70 family transcripts, heat-shock protein 70 cognate 5 and heat-shock protein 70 cognate 3 (designated as EsHSC70-5 and EsHSC70-3, respectively), were isolated from the Chinese mitten crab Eriocheir sinensis and their expression profiles were evaluated for their responsiveness to larval development and immune challenge in adul.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15036319
- DOI:
- 10.4238/gmr.15036319
Cloning and sequence analysis of an actin gene in aloe
Authors: S.S. Wen, D.W. He, C.M. Liao, J. Li, G.Q. Wen, and X.H. Liu
Aloe (Aloe spp), containing abundant polysaccharides and numerous bioactive ingredients, has remarkable medical, ornamental, calleidic, and edible values. In the present study, the total RNA was extracted from aloe leaf tissue. The isolated high-quality RNA was further used to clone actin gene by using reverse transcr.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.4.9
- DOI:
- 10.4238/2014.July.4.9