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Rna

Animal Genetics   Research Article

Lipopolysaccharide and �²-1,3-glucan binding protein in the hard clam (Meretrix meretrix): Molecular characterization and expression analysis

Authors: S.X. Liu, Z.H. Qi, J.J. Zhang, C.B. He, X.G. Gao and H.J. Li

Pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. In this study, a lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene was cloned from the hard clam Meretrix meretrix .. Read More»

Genet. Mol. Res. 13(3):
2014.July.4.10
DOI:
10.4238/2014.July.4.10
Human Genetics   Research Article

Detection of fetal RHD pseudogene (RHD�¨) and hybrid RHD-CE-Ds from RHD-negative pregnant women with a free DNA fetal kit

Authors: T. Gunel, I. Kalelioglu, A. Gedikbasi, H. Ermis and K. Aydinli

Hemolytic disease of the newborn is a clinical condition in which maternal and paternal Rh blood group antigens are incompatible and the mother is negative for the antigen whereas the father is positive. Analysis of fetal cells recovered from maternal plasma can provide a highly sensitive prenatal diagnosis. The fetal RHD gene.. Read More»

Genet. Mol. Res. 10(4):
2011.October.26.1
DOI:
10.4238/2011.October.26.1
Animal Genetics   Research Article

Changes in expression of specific miRNAs and their target genes in repair of exercise-induced muscle injury in rats

Authors: W. Wu

The effects of muscle-specific miRNAs in the repair of exercise-induced muscle injury were investigated by examining the changes in their expression and that of the target genes in rat skeletal muscle. Two-month-old agile male rats were randomly divided into exercise and static control groups, the former subdivided into 0-h, 6-h, 12-h, 1-day, 2-day, 3-day.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038698
DOI:
10.4238/gmr.15038698
Plant Genetics   Research Article

Physiological analysis and transcriptome comparison of two muskmelon (Cucumis melo L.) cultivars in response to salt stress

Authors: L.M. Wang, L.D. Zhang, J.B. Chen, D.F. Huang and Y.D. Zhang

Melon (Cucumis melo L.) is an important vegetable crop that ranks second in salt tolerance among the Cucurbitaceae. Previous studies on the two muskmelon cultivars ‘Bing XueCui’ (BXC) and ‘Yu Lu’ (YL) revealed that they had different characteristics under salt stress, but the molecular basis underlying their different physiological.. Read More»

Genet. Mol. Res. 15(3):
gmr.15038738
DOI:
10.4238/gmr.15038738
Cytogenomics   Research Article

CADM1 mRNA expression and clinicopathological significance in esophageal squamous cell carcinoma tissue

Authors: J.B. Qian, H.B. Liu, Y. Zhu, F. Lu, Q.C. Yang and Y. Shen

The mRNA expression of cell adhesion molecule 1 (CADM1) and its clinicopathological significance in esophageal squamous cell carcinoma (ESCC) tissues were investigated. CADM1 mRNA and protein expression were detected in tissue samples from 50 patients with ESCC by reverse transcription-polymerase chain reaction (RT-PCR) and st.. Read More»

Genet. Mol. Res. 16(2):
gmr16029178
DOI:
10.4238/gmr16029178
Human Genetics   Research Article

Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila

Authors: P.P. Wang, M. Ren, S.Q. Chen, S.W. Yin, C. Zhao, H.Y. Zhang, X.R. Li, Q.Q. Cao and G.Q. Zhou

Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequen.. Read More»

Genet. Mol. Res. 16(2):
gmr16029129
DOI:
10.4238/gmr16029129
Animal Genetics   Research Article

RNA-Seq-based transcriptome and the reproduction-related genes for the aphid Schlechtendalia chinensis (Hemiptera, Aphididae)

Authors: P. Liu, Z.X. Yang, X.M. Chen, P. Yang

Chinese galls form on sumac plants (Rhus chinensis) and are used for medicinal and chemical purposes, due to their richness in tannins. The galls are formed by aphids, the most prominent of which is Schlechtendalia chinensis, which forms horn-shaped galls on the winged rachis of R. chinensis. S. chinensis has a complex life cy.. Read More»

Genet. Mol. Res. 16(1):
gmr16019448
DOI:
10.4238/gmr16019448
Plant Genetics   Research Article

An effective method for extracting total RNA from Dioscorea opposita Thunb.

Authors: C.M. Liao, J. Li, X.H. Liu and Y.S. Zhang

Dioscorea opposita Thunb., included in the genus Dioscorea of the family Dioscoreaceae, is an important herb with great edible and medicinal value. In this study, the total RNA from leaves of Lichuan Dioscorea opposita Thunb. was isolated by an improved Trizol method. The results showed that the RNA extracted by the improved T.. Read More»

Genet. Mol. Res. 13(1):
2014.January.21.15
DOI:
10.4238/2014.January.21.15
Human Genetics   Research Article

Prognostic role of microRNA-100 in patients with bladder cancer

Authors: Y.H. Cao, H.H. Zhang,H.F. Xu, Y.J. Duan, Q. Li, B. Huang

We investigated the clinical significance and prognostic value of microRNA-100 (miR-100) in bladder cancer. Quantitative real-time polymerase chain reaction was used to analyze the expression of miR-100 in 92 pairs of human bladder cancer and adjacent normal tissue samples. Overall survival (OS) curves were plotted using the K.. Read More»

Genet. Mol. Res. 14(4):
2015.December.7.6
DOI:
10.4238/2015.December.7.6
Microbial Genetics   Research Article

A snapshot on prokaryotic diversity of the Solim�µes River basin (Amazon, Brazil)

Authors: D. Toyama, C.D. Santos-J�ºnior, L.T. Kishi, T.C.S. Oliveira, J.W. Garcia, H. Sarmento, F.P. Miranda and F. Henrique-Silva

The Amazon region has the largest hydrographic basin on the planet and âÂ?¨is well known for its huge biodiversity of plants and animals. However, âÂ?¨there is a lack of studies on aquatic microbial biodiversity in the âÂ?¨Solimões River, one of its main water courses. To investigate the âÂ?¨microbial biodiversi.. Read More»

Genet. Mol. Res. 16(2):
gmr16029567
DOI:
10.4238/gmr16029567