Rna
Responsivity to PGE2 labor induction involves concomitant differential prostaglandin E receptor gene expression in cervix and myometrium
Authors: C.K. Konopka, W.G. Glanzner, M.L. Rigo, M.T. Rovani, F.V. Comim, P.B.D. Gon�§alves, E.N. Morais, A.Q. Antoniazzi, C.F. Mello and I.B.M. Cruz
Prostaglandin E2 (dinoprostone) is largely used for labor induction. However, one-third of patients do not respond to treatment. One cause of this poor response may be associated with changes in regulation of prostaglandin E receptors (EP1-4). In this study, we investigated EP mRNA expression in the uterine cervix and lower uterine segment myometrium for .. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.9.25
- DOI:
- http://dx.doi.org/10.4238/2015.September.9.25
shRNA inhibits the expression of chicken telomerase reverse transcriptase in MDCC-MSB1 cells
Authors: N. Jiang, X.B. Zheng, Z.Y. Zhao, Z.G. Qin and T.J. Liu
Here, we investigated the effects of blocking chicken telomerase reverse transcriptase (chTERT) in MDCC-MSB1 cells, using small-hairpin RNAs (shRNAs) to interfere with gene expression. shRNAs specific to chTERT mRNA were designed, cloned into DNA plasmid vectors, and transfected into MDCC-MSB1 cells. The transfected chTERT RNAs were expressed by the RNA p.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016979
- DOI:
- 10.4238/gmr.15016979
Effects of VEGF suppression by small hairpin RNA interference combined with radiotherapy on the growth of cervical cancer
Authors: L. Qi, L.N. Xing, X. Wei and S.G. Song
This study aimed to investigate the inhibition of vascular endothelial growth factor (VEGF) by small hairpin RNA (shRNA) interference combined with radiotherapy on the growth of cervical cancer SiHa cell xenografts in nude mice. The effective pVEGF-shRNA plasmid was screened by reverse transcription-polymerase chain reaction (.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.7.2
- DOI:
- 10.4238/2014.July.7.2
Prognostic significance of long non-coding RNA MALAT-1 in various human carcinomas: a meta-analysis
Authors: J. Wang1*, A.M. Xu1*, J.Y. Zhang1*, X.M. He2, Y.S. Pan1, G. Cheng1, C. Qin1,L.X. Hua1 and Z.J. Wang1
The long non-coding RNA MALAT-1 plays an important role in cancer prognosis. The present research aimed to elucidate its precise predictive value in various human carcinomas. A quantitative meta-analysis was performed by searching PubMed, Embase, Web of Science, and Cochrane Library (most recently, January 2015) databases, and extracting data from studies.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017433
- DOI:
- 10.4238/gmr.15017433
E2F, HSF2, and miR-26 in thyroid carcinoma: bioinformatic analysis of RNA-sequencing data
Authors: J.C. Lu and Y.P. Zhang
In this study, we examined the molecular mechanism of thyroid carcinoma (THCA) using bioinformatics. RNA-sequencing data of THCA (N = 498) and normal thyroid tissue (N = 59) were downloaded from The Cancer Genome Atlas. Next, gene expression levels were calculated using the TCC package and differentially expressed genes (DEGs) were identified using the ed.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017576
- DOI:
- 10.4238/gmr.15017576
Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3
Authors: X. Liu*, L.G. Wang*, L.C. Zhang, H. Yan, K.B. Zhao, J. Liang, N. Li, L. Pu,T. Zhang and L.X. Wang
The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA end.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017010
- DOI:
- 10.4238/gmr.15017010
Tetrandrine induces microRNA differential expression in human hypertrophic scar fibroblasts in vitro
Authors: Tetrandrine induces microRNA differential expression in human hypertrophic scar fibroblasts in vitro
MicroRNAs (miRNAs) have recently been shown to play a role in normal wound healing process. miRNAs may be linked to pathologic wound healing and closely related to the formation of hypertrophic scars. This study aimed to explore the effects of tetrandrine on the miRNA expression profile in human hypertrophic scar fibroblasts (HSFs) in vitro. HSFs were ran.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017288
- DOI:
- 10.4238/gmr.15017288
Early diagnosis of a Mexican variant of Papaya meleira virus (PMeV-Mx) by RT-PCR
Authors: E. Zamudio-Moreno, J.H. Ramirez-Prado, O.A. Moreno-Valenzuela and L.A. Lopez-Ochoa
Papaya meleira disease was identified in Brazil in the 1980s. The disease is caused by a double-stranded RNA virus known as Papaya meleira virus (PMeV), which has also been recently reported in Mexico. However, previously reported PMeV primers failed to diagnose the Mexican form of the disease. A genomic approach was used to identify sequences of the Mexi.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.6.18
- DOI:
- 10.4238/2015.February.6.18
Tissue-specific adaptive levels of glucocorticoid receptor alpha mRNA and their relationship with insulin resistance
Authors: R.B. Castro C.A. Longui C.D.C. Faria T.S. Silva F. Richeti M.N. Rocha M.R. Melo W.L. Pereira E.G. Chamlian L.A. Rivetti
Insulin resistance is an underlying cause of metabolic changes associated with cardiovascular diseases. Glucocorticoids are known determinant factors of insulin resistance. We quantified glucocorticoid receptor alpha (GRÃ?±) mRNA and 11 beta-hydroxysteroid dehydrogenase type 1 (11Ã?²-HSD1) mRNA in various tissues of 35 patients with previously established .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.21.1
- DOI:
- 10.4238/2012.November.21.1
Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi
Authors: N. Ally X.L. Zou B.C. Jiang L. Qin L. Zhai P. Xiao H.L. Liu
Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonuc.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.28.1
- DOI:
- 10.4238/2012.November.28.1