Rna
Unraveling systematic inventory of Echinops (Asteraceae) with special reference to nrDNA ITS sequence-based molecular typing of Echinops abuzinadianus
Authors: M.A. Ali, F.M. Al-Hemaid, J. Lee, A.A. Hatamleh, G. Gyulai and M.O. Rahman
The present study explored the systematic inventory of Echinops L. (Asteraceae) of Saudi Arabia, with special reference to the molecular typing of Echinops abuzinadianus Chaudhary, an endemic species to Saudi Arabia, based on the internal transcribed spacer (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA. A sequence .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.2.9
- DOI:
- 10.4238/2015.October.2.9
Molecular cloning, expression and variation analyses of the dopamine D2 receptor gene in pig breeds in China
Authors: H.P. Xu, X.M. He, M.X. Fang, Y.S. Hu, X.Z. Jia, Q.H. Nie and X.Q. Zhang
The dopamine D2 receptor (DRD2) is a crucial mediator for normal physiological processes. We cloned the pig DRD2 gene, investigated its distribution in tissues and identified polymorphisms by RT-PCR, quantitative real-time PCR and direct sequencing. Two Yorkshire pigs from Guangdong Academy of Agricultural Sciences (Guangzhou,.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.5.6
- DOI:
- 10.4238/2011.December.5.6
Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)
Authors: S.A. Rhoden, A. Garcia, M.C. Santos e Silva, J.L. Azevedo and J.A. Pamphile
Various organisms such as fungi and bacteria can live inside plants, inhabiting the aerial parts (primarily the leaves) without causing damage. These microorganisms, called endophytes, produce an extensive variety of compounds that can be useful for medical and agronomic purposes. Trichilia elegans A. Juss., belonging to the Meliaceae family, shows wide d.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.20.7
- DOI:
- 10.4238/2015.February.20.7
Isolation of retro-transcribed RNA from in vitro Mycosphaerella fijiensis-infected banana leaves
Authors: C.M. RodrÃ?Âguez-GarcÃ?Âa, L. Peraza-EcheverrÃ?Âa, I.R. Islas-Flores, B.B. Canto-CanchÃ?© and R. Grijalva-Arango
High polyphenol and polysaccharide levels in plant tissues such as banana fruit and leaves constitute a significant challenge to the extraction of sufficient amounts of high-quality RNA required for cDNA library synthesis and molecular analysis. To determine their comparative effectiveness at eliminating polyphenols, polysacch.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr865
- DOI:
- 10.4238/vol9-3gmr865
Expressional analysis of immune-related miRNAs in breast milk
Authors: R.S. Na, G.X. E, W. Sun, X.W. Sun, X.Y. Qiu, L.P. Chen and Y.F. Huang
Immune-related miRNAs in breast milk are extracellular miRNAs that are related to immune organ development and regulation of the immune function in infants and young animals. The goal of this study was to compare the expression levels of five immune-related miRNAs in breast milk in black goats, humans, and dairy cattle. The miRNAs from milk were extracted.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.25.4
- DOI:
- http://dx.doi.org/10.4238/2015.September.25.4
Comparison of bacterial communities in the Solim�µes and Negro River tributaries of the Amazon River based on small subunit rRNA gene sequences
Authors: J.C.C. Peixoto, L. Leomil, J.V. Souza, F.B.S. Peixoto and S. Astolfi-Filho
The microbiota of the Amazon River basin has been little studied. We compared the structure of bacterial communities of the Solimões and Negro Rivers, the main Amazon River tributaries, based on analysis of 16S rRNA gene sequences. Water was sampled with a 3-L Van Dorn collection bottle; samples were collected at nine d.. Read More»
- Genet. Mol. Res. 10(4):
Genomic structure, polymorphism and expression analysis of growth hormone-releasing hormone and pituitary adenylate cyclase activating polypeptide genes in the half-smooth tongue sole (Cynoglossus semilaevis)
Authors: Q. Ma, S.F. Liu, Z.M. Zhuang, L. Lin, Z.Z. Sun, C.L. Liu, Y.Q. Su and Q.S. Tang
Growth hormone-releasing hormone (GHRH) and pituitary adenylate cyclase activating polypeptide (PACAP) regulate development and somatic growth in teleosts; they may be associated with sexual growth dimorphism in the half-smooth tongue sole (Cynoglossus semilaevis). We found that the full-length GHRH and PACAP gene sequences ob.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.12.1
- DOI:
- 10.4238/2011.December.12.1
Identification of critical TF-miRNA-mRNA regulation loops for colorectal cancer metastasis.
Authors: C. Wang, D.Z. Hu and J.Z. Liu
To explore the potential cause of colorectal cancer metastasis, gene expression profiles, GSE21510, and miRNA expression profiles, GSE48074, were downloaded from the Gene Expression Omnibus database. Differentially expressed genes in metastatic colorectal and non metastatic colorectal cancer compared with the normal samples were identified via the limma p.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.19
- DOI:
- 10.4238/2015.May.22.19
Identification of critical TF-miRNA-mRNA regulation loops for colorectal cancer metastasis
Authors: C. Wang D.Z. Hu and J.Z. Liu
To explore the potential cause of colorectal cancer metastasis, gene expression profiles, GSE21510, and miRNA expression profiles, GSE48074, were downloaded from the Gene Expression Omnibus database. Differentially expressed genes in metastatic colorectal and non metastatic colorectal cancer compared with the normal samples were identified via the limma p.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.19
- DOI:
- 10.4238/2015.May.22.19
MicroRNAs as novel biomarkers for the differentiation of malignant versus benign thyroid lesions: a meta-analysis
Authors: G.J. Zhou, M. Xiao, L.N. Zhao, J.G. Tang and L. Zhang
The aim of this meta-analysis was to systematically evaluate the diagnostic accuracy of microRNAs (miRNAs) in distinguishing malignant thyroid lesions from benign ones and to determine the potential of miRNAs as diagnostic biomarkers for thyroid cancer. The random-effect model was used to summarize the pooled estimates of diagnostic accuracy, including se.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.3
- DOI:
- 10.4238/2015.July.3.3