Rna
Characterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing
Authors: H. Shen, H.Y. Chen, B. Jia, G.H. Han, Y.S. Zhang and X.C. Zeng
The role of microRNAs (miRNAs) in the regulation of mammalian reproduction has been demonstrated previously. However, only a few studies have assessed the role of miRNAs in the reproduction processes of sheep. The elucidation of miRNA expression profiles in the ovaries of different sheep breeds representing fecundity extremes will be useful in understandi.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.11
- DOI:
- 10.4238/2015.July.3.11
Implications of microRNA-197 downregulated expression in esophageal cancer with poor prognosis
Authors: T.-Y. Wang, S.-G. Liu, B.-S. Zhao, B. Qi, X.-G. Qin and W.-J. Yao
The aim of this study was to investigate the significance of the microRNA miR-197 expression level in relation to clinicopathological factors and prognoses of esophageal cancer (EC). MicroRNA was extracted using the Taqman® MicroRNA Assay from 46 EC patients at the same tumor node metastasis (TNM) stage, but with different.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.12
- DOI:
- 10.4238/2014.July.25.12
Construction and identification of pIRES2-NGF-VEGF165 bicistronic eukaryotic expression vector
Authors: B.N. Li, W.D. Li, H.G. Feng, J.T. Lin and Z.Q. Yuan
We used a simple and efficient method to construct the bicistronic eukaryotic expression vector pIRES2-NGF-VEGF165. The nerve growth factor (NGF) gene was obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The NGF cDNA fragment was inserted into the multiple cloning sites of the pI.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.25.23
- DOI:
- 10.4238/2014.July.25.23
Two splice variants of the bovine lactoferrin gene identified in Staphylococcus aureus isolated from mastitis in dairy cattle
Authors: J.M. Huang, Z.Y. Wang, Z.H. Ju, C.F. Wang, Q.L. Li, T. Sun, Q.L. Hou, S.Q. Hang, M.H. Hou and J.F. Zhong
Bovine lactoferrin (bLF) is a member of the transferrin family; it plays an important role in the innate immune response. We identified novel splice variants of the bLF gene in mastitis-infected and healthy cows. Reverse transcription-polymerase chain reaction (RT-PCR) and clone sequencing analysis were used to screen the spli.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.December.21.1
- DOI:
- 10.4238/2011.December.21.1
Isolation and characterization of yeasts capable of efficient utilization of hemicellulosic hydrolyzate as the carbon source
Authors: L.A. Cassa-Barbosa, R.E.L. Proc�³pio, I.T.S.R. Matos and S.A. Filho
Few yeasts have shown the potential to efficiently utilize hemicellulosic hydrolyzate as the carbon source. In this study, microorganisms isolated from the Manaus region in Amazonas, Brazil, were characterized based on their utilization of the pentoses, xylose, and arabinose. The yeasts that showed a potential to assimilate these sugars were selected for .. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.28.12
- DOI:
- http://dx.doi.org/10.4238/2015.September.28.12
Protein interaction and microRNA network analysis in osteoarthritis meniscal cells
Authors: M. Wang, C. Liu, Y. Zhang, Y. Hao, X. Zhang and Y.M. Zhang
Osteoarthritis is the most common form of arthritis among elderly adults. Herein, we performed protein-protein interaction (PPI) and miRNA network analysis to evaluate the global correlation between miRNA regulation and the PPI network in human osteoarthritis. Our results showed that desmoplakin (DSP), cystatin A (CSTA), calmodulin 1, tyrosine kinase endo.. Read More»
- Genet. Mol. Res. 12(1):
- 2013
- DOI:
- 10.4238/2013
Endophytic bacteria from Piper tuberculatum Jacq.: isolation, molecular characterization, and in vitro screening for the control of Fusarium solani f. sp piperis, the causal agent of root rot disease in black pepper (Piper nigrum L.)
Authors: S.B. Nascimento, A.M. Lima, B.N. Borges and C.R.B. de Souza
Endophytic bacteria have been found to colonize internal tissues in many different plants, where they can have several beneficial effects, including defense against pathogens. In this study, we aimed to identify endophytic bacteria associated with roots of the tropical piperaceae Piper tuberculatum, which is known for itsresistance to infection by Fusariu.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.3.32
- DOI:
- 10.4238/2015.July.3.32
Multibreed genetic evaluation in bovines using simulated data employing a composite population
Authors: A.L. Bocchi, H.N. Oliveira, J.B.S. Ferraz and J.P. Eler
Genetic evaluations in Brazil are performed within each animal breed; however, with the wide range of extant genetic groups in the country and the increased use of genetic crossing as a form of rapid meat production, more elaborate programs that can jointly evaluate animals of different genetic groups are needed. Genetic evalu.. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048382
- DOI:
- 10.4238/gmr.15048382
Analysis of blood processing conditions to obtain high-quality total RNA from human leukocyte concentrate
Authors: Perez IAG, Santana SP, Argudin TD and Gardon DOP
Blood samples are used as a biological source to discover biomarkers of hematological and non-hematological disorders. The present study shows the impact of different experimental conditions associated with cell lysis buffer, TRI-reagent protocol and blood cell storage buffer and their correlation with the quantity, quality an.. Read More»
- Genet. Mol. Res. 6(2):
Identification of aac(2')-I Type b aminoglycoside-modifying enzyme genes in resistant Acinetobacter baumannii
Authors: T. Lin, C.G. Tang, Q.H. Li, J. Ji, H.Y. Ge, X.Y. Zhang and H.P. Sun
The aim of this study was to investigate the mechanism underlying the drug resistance of Acinetobacter baumannii toward aminoglycosides. A total of 32 A. baumannii strains were identified by molecular identification and subsequently isolated. The isolates were then amplified by polymerase chain reaction to analyze the 9 aminoglycoside-modifying enzyme gen.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.13.11
- DOI:
- 10.4238/2015.March.13.11