Rna
Involvement of AP-1 in p38MAPK signaling pathway in osteoblast apoptosis induced by high glucose
Authors: Z.P. Feng, H.C. Deng, R. Jiang, J. Du and D.Y. Cheng
We investigated the effect of p38MAPK/AP-1 (activator protein-1) signaling on the apoptosis of osteoblasts induced by high glucose. A lentivirus vector of small hairpin RNA (shRNA) targeting p38MAPK was constructed in vitro. Osteoblasts MC3T3-E1 cultured in vitro were treated with vehicle, high glucose, p38MAPK-shRNA transfection, p38MAPK inhibitor, and u.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.26
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.26
Effects of AFP gene silencing on Survivin mRNA expression inhibition in HepG2 cells
Authors: Z.L. Fang, N. Fang, X.N. Han, G. Huang, X.J. Fu, G.S. Xie, N.R. Wang and J.P. Xiong
We investigated the effects of alpha-fetoprotein (AFP) gene silencing on Survivin expression in HepG2 cells. Small interfering RNA technology was used to downregulate AFP expression in HepG2 cells. An enzyme-linked immunosorbent assay was used to measure AFP concentration in the supernatant before and after transfection. An MTT assay was used to detect ce.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.30
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.30
Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake
Authors: L. Pu, L.C. Zhang, J.S. Zhang, X. Song, L.G. Wang, J. Liang, Y.B. Zhang, X. Liu, H. Yan, T. Zhang, J.W. Yue, N. Li, Q.Q. Wu and L.X. Wang
Mitogen-activated protein kinase kinase kinase 5 (MAP3K5) is essential for apoptosis, proliferation, differentiation, and immune responses, and is a candidate marker for residual feed intake (RFI) in pig. We cloned the full-length cDNA sequence of porcine MAP3K5 by rapid-amplification of cDNA ends. The 5451-bp gene contains a 5'-untranslated region (UTR) .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037998
- DOI:
- 10.4238/gmr.15037998
14-3-3 Gene expression in regenerating rat liver after 2/3 partial hepatectomy
Authors: D.M. Xue, X.Q. Guo, R. Chen, Z.P. Niu and C.S. Xu
14-3-3 Proteins are a ubiquitous family of molecules that participate in protein kinase signaling pathways in all eukaryotic cells. Functioning as phosphoserine/phosphothreonine-binding modules, 14-3-3 proteins participate in the phosphorylation-dependent protein-protein interactions that control progression through the cell cycle, initiation and.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.20.12
- DOI:
- 10.4238/2015.March.20.12
Characterization and comparison of flower bud microRNAs from yellow-horn species
Authors: Y. Ao
An increasing number of microRNAs (miRNAs) are known to play crucial regulatory roles in floral organ differentiation and development. Yellow-horn (Xanthoceras sorbifolia Bunge) is an oil-rich seed shrub that has great potential for biodiesel production. To evaluate the roles of miRNAs in the regulation of flower development, .. Read More»
- Genet. Mol. Res. 15(4):
- gmr.15048899
- DOI:
- 10.4238/gmr.15048899
Source of bacterial RNA in chronic otitis media with effusion
Authors: P.-Z. Li, L. Cheng and M.-L. Qiu
The purpose of this study was to investigate whether the bacterial RNA detected by polymerase chain reaction (PCR) and reverse transcription (RT)-PCR methods in middle ear effusion (MEE) for pediatric chronic otitis media with effusion (OME) originated from live bacteria. Degradation of RNA was observed by spectroscopic analys.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.24
- DOI:
- 10.4238/2014.August.7.24
Expression of microRNA-106b and its clinical significance in cutaneous melanoma
Authors: N. Lin, Y. Zhou, X. Lian and Y. Tu
MicroRNA-106b (miR-106b) is overexpressed in various types of cancers and is associated with the regulation of carcinogenic processes. However, its clinical significance in cutaneous melanoma has not been reported. qRT-PCR was performed to examine the expression of miR-106b in 15 cases of dysplastic nevi, 17 cases of melanoma metastases, and 97 cases of p.. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Differential gene expression of epigenetic modifying enzymes between Tibet pig and Yorkshire in high and low altitudes
Authors: D.M. Ban, B. Zhang, Z.X. Wang, H. Zhang and C.X. Wu
Epigenetic modifying enzymes play important roles in the adaptation to hypoxia, although no studies have examined their expression levels in Tibet pigs. The lung is an important functional organ in hypoxia adaptation. In this study, we examined the mRNA expression level of 5 enzymes in the lung of Tibet pigs using real-time polymerase chain reaction to de.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.13.6
- DOI:
- http://dx.doi.org/10.4238/2015.April.13.6
Hypoxia-inducible factor-2�± (HIF-2�±) mediates the effects of hypoxia on the promotion of HeLa cell viability, colony formation, and invasion capacity in vitro
Authors: J. Xiong, F.F. Zhu and M.F. Nie
Hypoxia reduces the oxygen supply to tumor cells and may limit tumor cell growth. However, hypoxia promotes tumor cell metabolic adaptation, apoptosis resistance, angiogenesis, invasion, and metastasis. Hypoxia-inducible factor-2a (HIF-2a) may be responsible for these hypoxia-induced changes. In this study, we investigated the effects of hypoxia and HIF-2.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.13.7
- DOI:
- http://dx.doi.org/10.4238/2015.April.13.7
Association of miR-196a2, miR-27a, and miR-499 polymorphisms with isolated congenital heart disease in a Chinese population
Authors: K. Yu, Y. Ji, H. Wang, Q.K. Xuan, B.B. Li, J.J. Xiao, W. Sun and X.Q. Kong
We hypothesized that single nucleotide polymorphisms (SNPs) in certain microRNAs contribute to congenital heart disease (CHD) phenotypes. Five hundred and seventy-three subjects were enrolled in this study. DNA extracted from peripheral blood cells was used for SNP genotyping of miR-196a2 (rs11614913), miR-27a (rs11671784, rs8.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048929
- DOI:
- 10.4238/gmr15048929