Rna
Benchmark comparison of ab initio microRNA identification methods and software
Authors: L.L. Hu Y. Huang Q.C. Wang Q. Zou Y. Jiang
MicroRNAs (miRNAs) are short, non-coding RNA molecules that play an important role in the world of genes, especially in regulating the gene expression of target messenger RNAs through cleavage or translational repression of messenger RNA. Ab initio methods have become popular in computational miRNA detection. Most software tools are designed to distinguish m.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.17.4
- DOI:
- 10.4238/2012.October.17.4
Dephosphorylation of NSSR1 regulates alternative splicing of the GluR-B minigene
Authors: W. Zhang, L.L. Li, X.H. Chen and Z.Y. Peng
Neural salient serine/arginine-rich protein 1 (NSSR1, alternatively SRp38) is an important splicing factor that can repress pre-mRNA alternative splicing in cells during heat shock and mitosis. We show here that NSSR1 protein is dephosphorylated when cells are heat shocked or incubated with kinase inhibitor K252a. Both heat sh.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.3
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.3
Molecular cloning and sequence analysis of follicle-stimulating hormone beta polypeptide precursor cDNA from the bovine pituitary gland
Authors: L.S. Dai, Y.M. Zhao, G.L. Zhang, R.F. Zhao, H. Jiang, T.H. Ma, Y. Gao, B. Yuan, Y.L. Xu, W.Y. Yu, Z.H. Zhao and J.B. Zhang
Follicle-stimulating hormone (FSH) plays an essential role in mammalian spermatogenesis and follicular development. In a previous study, we demonstrated that some bulls carry numerous linked mutations in the FSH beta-subunit (FSHB) gene, and that these bulls have poor-quality semen, low fertility, and slightly lower serum FSH .. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr997
- DOI:
- 10.4238/vol10-3gmr997
Heteroduplex formation and S1 digestion for mapping alternative splicing sites
Authors: E.N. Ferreira, M.C.R. Rangel, P.B. Pineda, D.O. Vidal, A.A. Camargo, S.J. Souza and D.M. Carraro
The identification of alternatively spliced transcripts has contributed to a better comprehension of developmental mechanisms, tissue-specific physiological processes and human diseases. Polymerase chain reaction amplification of alternatively spliced variants commonly leads to the formation of heteroduplexes as a result of ba.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3x-meeting012
- DOI:
- 10.4238/vol7-3x-meeting012
MicroRNA-338-3p inhibits glucocorticoid-induced osteoclast formation through RANKL targeting
Authors: X.H. Zhang, G.L. Geng, B. Su, C.P. Liang, F. Wang and J.C. Bao
The differentiation deficiencies of osteoclast precursors (pre-OCs) may contribute to osteoporosis. Research on osteoporosis has recently focused on microRNAs (miRNAs) that play crucial roles in pre-OC differentiation. In the current study, we aimed to analyze the expression and function of the glucocorticoid (GC)-associated miRNA-338-3p (miR-338-3p) in o.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037674
- DOI:
- 10.4238/gmr.15037674
Application of RNA-seq to reveal the transcript profile in bacteria
Authors: A.C. Pinto, H.P. Melo-Barbosa, A. Miyoshi, A. Silva and V. Azevedo
The large number of microbial genomes deposited in databanks has opened the door for in-depth studies of organisms, including post-genomics investigations. Thanks to new generation sequencing technology, these studies have made advances that have lead to extraordinary discoveries in bacterial transcriptomics. In this review, w.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1554
- DOI:
- 10.4238/vol10-3gmr1554
Induction function of siRNA-mediated survivin gene silencing on nasopharyngeal carcinoma cell apoptosis
Authors: S.M. Fu, Z.H. Tu, L.Q. Deng, J.H. Cai, Z. Liang, Z.Q. Lin and Y.T. Wang
We examined the function of survivin gene expression in patients with nasopharyngeal carcinoma (NPC), as well as small interfering RNA (siRNA) on controlling CNE-2 NPC proliferation and apoptosis. Immunohistological methods, in situ hybridization, and reverse transcription-polymerase chain reaction technique were used to detect survivin protein and mRNA e.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.30.12
- DOI:
- http://dx.doi.org/10.4238/2015.March.30.12
Molecular cloning and characterization of a novel Y-box gene from Sepiella maindroni
Authors: H.P. Si, C.L.Wang, Y.Y. Zhang, C.K. Mu, P.P. Zhan, R.H. Li and W.W. Song
Y-box proteins are a family of highly conserved nucleic acid binding proteins that interact with genome and transcription product to modulate the transcriptional and translational processes. In the present study, a complete mRNA of Y-box binding protein (designated SmYB) was obtained from Sepiella maindroni by amplification of flanking sequences. The full.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.7
- DOI:
- 10.4238/2015.May.29.7
Hsp110 expression changes in rat primary myocardial cells exposed to heat stress in vitro
Authors: Z.J. Liu, Y.J. Lv, M. Zhang, Z.H. Yue, S. Tang, A. Islam, B. Rehana, E.D. Bao and J. Hartung
We investigated and described the kinetics of heat shock protein (Hsp) 110 expression and distribution in rat primary myocardial cells exposed to heat stress in vitro. After incubation at 37°C for 72 h, myocardial cells were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min. Significant increases .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.29.1
- DOI:
- 10.4238/2012.November.29.1
Kallikrein 12 downregulation reduces AGS gastric cancer cell proliferation and migration
Authors: X.S. Li and X.L. He
Abnormal expression of the kallikrein (KLK) family of serine proteases closely correlates with onset, progression, and prognosis of endocrine gland-related malignant tumors. The aim of this study was to evaluate how downregulation of KLK12 influenced cell cycle and proliferation of the AGS gastric cancer cell line. KLK12 was d.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038452
- DOI:
- 10.4238/gmr.15038452