Rna
mRNA expression of prolactin receptor in sheep pituitary and its effect on maternal behavior
Authors: L.P. Wang
Prolactin receptor (PRLR) is the specific receptor for prolactin. Brain-specific control of PRLR gene expression is correlated with the induction of maternal behavior. The aim of this study was to investigate the dynamic changes in the PRLR mRNA level in the sheep pituitary gland and its effect on ewe maternal behavior. The mRNA expression of PRLR during .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.31.13
- DOI:
- 10.4238/2015.July.31.13
Downregulation of microRNA-630 inhibits cell proliferation and invasion and enhances chemosensitivity in human ovarian carcinoma
Authors: Y.T. Zou, J.Y. Gao, H.L. Wang, Y. Wang, H. Wang and P.L. Li
MicroRNAs (miRNAs) are a family of small non-coding RNAs (approximately 21-23 nt long) that can target genes for either degradation of mRNA or inhibition of translation. miRNAs have not been comprehensively studied in human epithelial ovarian carcinoma (EOC). MicroRNA-630 (miR-630) has been frequently observed to be aberrantly expressed in various types o.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.31.25
- DOI:
- 10.4238/2015.July.31.25
DNase concentration assay to obtain DNA-free RNA from sugarcane leaves
Authors: J.A. Santos, G.A. Luz, K.P. Oliveira, L.F. Oliveira, A.S. Andrade J�ºnior, S.E.S. Valente and P.S.C. Lima
The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (<1%) can produce a false positive amplification signal in quantitative real-time PCR. For RNA contaminated with .. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049073
- DOI:
- 10.4238/gmr15049073
Effects of NELL2 on the regulation of GnRH expression and puberty in female rats
Authors: S.S. Zhou and P. Li
Neural tissue-specific epidermal growth factor-like repeat domain-containing protein (NELL2) was previously found to play an important role in nerve growth, neural differentiation, neural elasticity, synaptic transport, and vesicle release. In this study, we examined the effect of NELL2 on gonadotropin-releasing hormone (GnRH).. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.12
- DOI:
- 10.4238/2014.August.28.12
Efficient delivery of connective tissue growth factor shRNA using PAMAM nanoparticles
Authors: Z.J. Huang, B. Yi, H. Yuan and G.P. Yang
The aim of this study was to detect the anti-fibrosis activity of connective tissue growth factor (CTGF) small hairpin RNA (shRNA) mediated by polyamidoamine dendrimer nanoparticles in rat myocardial cell lines and myocardium. CTGF shRNAs were constructed from inverted oligonucleotides and a polyamidoamine nanoparticle vector .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.15
- DOI:
- 10.4238/2014.August.28.15
Inhibitory effect of survivin-targeting small interfering RNA on gastric cancer cells
Authors: Y.H. Li, M. Chen, M. Zhang, X.Q. Zhang, S. Zhang, C.G. Yu, Z.M. Xu and X.P. Zou
A pair of inverted repeated sequences of the gene survivin was designed for stable double-stranded RNA establishment. After stable transfection, the biological behaviors of gastric cancer cells were observed. The interference rates of survivin-targeting siRNA (siRNA-survivin) in BGC823, MKN45, SGC7901, and ci.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.22
- DOI:
- 10.4238/2014.August.28.22
Identification of the CAD gene from Eucalyptus urophylla GLU4 and its functional analysis in transgenic tobacco
Authors: B.W. Chen, Y.F. Xiao, J.J. Li, H.L. Liu, Z.H. Qin, Y. Gai and X.N. Jiang
Cinnamyl alcohol dehydrogenase (CAD) catalyzes the final step in lignin biosynthesis. The genus Eucalyptus belongs to the family Myrtaceae, which is the main cultivated species in China. Eucalyptus urophylla GLU4 (GLU4) is widely grown in Guangxi. It is preferred for pulping because of its excellent cellulose content and fiber.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049062
- DOI:
- 10.4238/gmr15049062
Expression and distribution of SP and its NK1 receptor in the brain-gut axis in neonatal maternally separated rat model with visceral hypersensitivity
Authors: W. Teng, H. Chen, F. Guo, X. Du, X. Fu, Y. Fang, H. Zhang, M. Fang and M. Ding
Neurokinin-1 receptor (NK1R) is a high affinity Substance P (SP) receptor and plays a key role in visceral hypersensitivity in irritable bowel syndrome (IBS). Early life stress is a significant risk factor in IBS. The aim of the present study was to investigate the influence of neonatal maternal separation on the expression and distribution of SP and its .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038999
- DOI:
- 10.4238/gmr.15038999
Drosha, DGCR8, and Dicer mRNAs are downregulated in human cells infected with dengue virus 4 - Genet. Mol. Res. 15 (2): gmr.15027891 Drosha, Dicer, and TRBP mRNA are downregulated in Vero cells with the 3 UTR of Dengue virus
Authors: J.A. Castillo and S. Urcuqui-Inchima
A recent paper (Casseb et al., 2016) published in the journal Genetics and Molecular Research described the interesting concept that dengue virus (DENV)-4 infection, in the human cell line A-549, leads to the downregulation of expression of key components of microRNA (miRNA) biogenesis, such as Drosha, Dicer, and DGCR8. For th.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15039050
- DOI:
- 10.4238/gmr.15039050
Construction and identification of pIRES2-LIF-NT-3 bicistronic eukaryotic expression vector
Authors: B.N. Li, W.D. Li*, J.T. Lin, H.G. Feng and Z.Q. Yuan
We used a simple and efficient method to construct a bicistronic eukaryotic expression vector pIRES2-LIF-NT-3. The leukemia inhibitory factor (LIF) and neurotrophin-3 (NT-3) genes were obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The LIF cDNA fragment was inserted into.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.June.18.12
- DOI:
- 10.4238/2014.June.18.12