Rna
Discovery of differentially expressed genes in cashmere goat (Capra hircus) hair follicles by RNA sequencing
Authors: X. Qiao, J.H. Wu,, R.B. W, R. Su, C. Li, Y.J. Zhang1, R.J. Wang, Y.H. Zhao, Y.X. Fan, W.G. Zhang and J.Q. Li
The mammalian hair follicle (HF) is a unique, highly regenerative organ with a distinct developmental cycle. Cashmere goatCapra hircus) HFs can be divided into two categories based on structure and development time: primary and secondary follicles. To identify differentially expressed genes (DEGs) in the primary and secondary .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038589
- DOI:
- 10.4238/gmr.15038589
Construction and identification of pIRES2-VEGF165-NT-3 bicistronic eukaryotic expression vector
Authors: B.N. Li, W.D. Li, H.G. Feng, J.T. Lin and Z.Q. Yuan
We used a simple and efficient method to construct the bicistronic eukaryotic expression vector pIRES2-VEGF165-NT-3. The neurotrophin-3 (NT-3) gene was obtained from the genomic DNA of human peripheral blood mononuclear cells by polymerase chain reaction. The NT-3 cDNA fragment was cloned into the pIRES2-VEGF165-EGFP vector in.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.June.18.13
- DOI:
- 10.4238/2014.June.18.13
RNA interference-mediated silencing of the starch branching enzyme gene improves amylose content in rice
Authors: H.Y. Jiang, J. Zhang, J.M. Wang, M. Xia, S.W. Zhu and B.J. Cheng
Amylose and amylopectin are the 2 major components of plant storage starch. The rice starch branching enzyme (RBE) plays an important role in the starch components of rice. In the present study, we selected a specific 195-bp segment from the RBE3 gene to construct hairpin DNA, which was driven by an endosperm-specific high molecular weight glutenin promot.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.19
- DOI:
- 10.4238/2013.January.4.19
Effects of miR-27a upregulation on thyroid cancer cells migration, invasion, and angiogenesis
Authors: Y.L. Wang, W.G. Gong and Q.L. Yuan
Thyroid cancer is the most common type of endocrine tumor. MicroRNAs (miRNAs) play a critical role in a variety of diseases, especially cancer occurrence and progression. However, the specific mechanism by which miRNAs trigger disease states has not been fully elucidated. This study aims to investigate the role of miR-27a in t.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049070
- DOI:
- 10.4238/gmr15049070
Lack of effect of maternal body mass index on anthropometric characteristics of newborns in twin gestations
Authors: Gloria M.D.D. Colletto and Concei�§�£o A.M. Segre
We examined the correlation between maternal prepregnancy body mass index (BMI) and newborn weight, length, BMI, and gestational order, in singleton and twin births. The sample comprised 381 mothers of multiple babies (562 twins), and 7979 singleton pregnancies, used as controls. The Mann-Whitney non-parametric test was used t.. Read More»
- Genet. Mol. Res. 4(1):
Transcriptome analysis of peach (Prunus persica L. Batsch) during the late stage of fruit ripening
Authors: H.F. Pan, Y. Sheng, Z.H. Gao, H.L. Chen, Y.J. Qi, X.K. Yi, G.H. Qin and J.Y. Zhang
Fruit ripening is a complex developmental process, the details of which remain largely unknown in fleshy fruits. In this paper, the fruit flesh of two peach varieties, “Zhongyou9” (a nectarine; Prunus persica L. Batsch) and its mutant “Hongyu”, was analyzed by RNA-seq technology during two stages of rip.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049335
- DOI:
- 10.4238/gmr15049335
Identification and characterization of conserved microRNAs and their target genes in wheat (Triticum aestivum)
Authors: Z.J. Yin and F.F. Shen
MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression by translational repression or transcript degradation. A large number of miRNAs have been identified from model plant species; however, the character of conserved miRNAs is poorly understood. We studied 42 miRNA families that are conserved within the pl.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr805
- DOI:
- 10.4238/vol9-2gmr805
Characteristics of synonymous codon usage bias in the beginning region of West Nile virus
Authors: X.X. Ma*, Y.P. Feng*, J.L. Liu, L. Chen, Y.Q. Zhao, P.H. Guo, J.Z. Guo, L.Y. Ma, and Z.R. Ma
Adaptation in the overall codon usage pattern of West Nile virus (WNV) to that of two hosts was estimated based on the synonymous codon usage value (RSCU). Synonymous codon usage biases for the beginning coding sequence of this virus were also analyzed by calculating the usage fluctuation for each synonymous codon along the ta.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.5.13
- DOI:
- 10.4238/2014.September.5.13
Association between a functional variant in microRNA-27a and susceptibility to colorectal cancer in a Chinese Han population
Authors: Y. Cao, J. Hu, Y. Fang, Q. Chen and H. Li
Polymorphisms in pri-, pre-, and mature-microRNAs (miRNAs) have been proposed to be associated with various human cancers. Common single nucleotide polymorphisms (SNPs) in miRNA genes can influence the maturation of miRNAs or miRNA-mediated transcriptional regulation. Through genotyping the rs895819 SNP in 254 colorectal cance.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.8
- DOI:
- 10.4238/2014.September.12.8
Associations of miRNA polymorphisms and expression levels with breast cancer risk in the Chinese population
Authors: P. Qi, L. Wang, B. Zhou, W.J. Yao, S. Xu, Y. Zhou and Z.B. Xie
Single-nucleotide polymorphisms in microRNAs (miRNAs) may dramatically affect gene expression and subsequently alter individual susceptibility to cancer, and thus has become a research hotspot for many cancer types, including breast cancer. We recruited 321 breast cancer patients and 290 controls in our study. Four established miRNA single-nucleotide poly.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.2
- DOI:
- 10.4238/2015.June.11.2