Rna
Effects of monomethoxypolyethylene glycol-chitosan nanoparticle-mediated dual silencing of livin and survivin genes in prostate cancer PC-3M cells
Authors: A.Q. Yang, P.J. Wang, T. Huang, W.L. Zhou and J. Landman
Monomethoxypolyethylene glycol-chitosan (mPEG-CS) nanoparticles were used as interfering RNA carriers to transfect human prostate cancer PC-3M cells to evaluate the effects of livin and survivin gene silencing on the proliferation and apoptosis. mPEG-CS nanoparticles with sizes of approximately 60 nm were first synthesized by .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027430
- DOI:
- 10.4238/gmr.15027430
Prognostic significance of microRNA-10b overexpression in breast cancer: a meta-analysis
Authors: N. Wang, P. Chen, L.P. Huang and T.Z. Wang
Many microRNAs (miRNAs) exhibit altered expression levels in cancers, and they may be considered as valuable prognostic biomarkers for cancers. Here we aimed to summarize the recent advances in miR-10b involvement in human breast cancer and analyze the predicting role of miR-10b for survival. We searched, Embase, and Wanfang d.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027350
- DOI:
- 10.4238/gmr.15027350
Deep RNA sequencing elucidates microRNA-regulated molecular pathways in ischemic cardiomyopathy and nonischemic cardiomyopathy
Authors: X. Li, C.Y. Liu, Y.S. Li, J. Xu, D.G. Li, X.I. Li and D. Han
Deregulation of cardiac miRNA gene-regulatory networks is a feature of different heart diseases, including ischemic (ICM) and nonischemic (NICM) cardiomyopathy. Here, based on the paired miRNA and mRNA expression profiles in ICM and NICM, we identified the differentially expressed miRNAs and mRNAs and the expression signatures.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027465
- DOI:
- 10.4238/gmr.15027465
Efficient use of artificial micro-RNA to downregulate the expression of genes at the post-transcriptional level in Arabidopsis thaliana
Authors: A. Ud-Din, M. Rauf, S. Ghafoor, M.N.K. Khattak, M.W. Hameed, H. Shah, S. Jan, K. Muhammad, A. Rehman and Inamullah
icro-RNAs are cellular components regulating gene expression at the post-transcription level. In the present study, artificial micro-RNAs were used to decrease the transcript level of two genes, AtExpA8 (encoding an expansin) and AHL25 (encoding an AT-hook motif nuclear localized protein) in Arabidopsis thaliana. The backbone .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027439
- DOI:
- 10.4238/gmr.15027439
AMFR gene silencing inhibits the differentiation of porcine preadipocytes
Authors: C.Z. Chen, Y.N. Zhu, M.L. Chai, L.S. Dai, Y. Gao, H. Jiang, L.J. Zhang, Y. Ding, S.Y. Liu, Q.Y. Li, W.F. Lu and J.B. Zhang
Our study clarifies the role of the autocrine motility factor receptor (AMFR) gene in porcine preadipocyte differentiation. AMFR-siRNA was transfected into porcine preadipocytes and the preadipocytes were induced to differentiation. Subsequently, qRT-PCR was conducted to examine changes in mRNA expression of a series of genes .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027354
- DOI:
- 10.4238/gmr.15027354
Association of Beclin-1 and microRNA-30a expression with the severity and treatment response of colorectal cancer
Authors: L. Liu, T. Meng, Q.S. Wang, H.Z. Jin, Z.Q. Sun, B. Jin, F. Fang and H.J. Wang
We investigated the associations between Beclin-1 and microRNA-30a (miR-30a) expression and the severity and treatment response in colorectal cancer (CRC). Our sample size consisted of 139 CRC patients who were treated with surgery alone. Immunohistochemistry was used to investigate the expression and prognostic significance o.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027704
- DOI:
- 10.4238/gmr.15027704
Molecular characterization and expression analysis of the �²-actin gene from the ridgetail white prawn Exopalaemon carinicauda
Authors: J.P. Liang, Y. Wang, Q.Q. Ge, J.T. Li, P. Liu, J. Li and G.X. Nie
Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ecβ-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ecβ-actin was .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026872
- DOI:
- 10.4238/gmr.15026872
MicroRNA-199a-3p is downregulated in gastric carcinomas and modulates cell proliferation
Authors: W. Peng, Z.-Y. Chen, L. Wang, Z. Wang and J. Li
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the translation of targeted mRNAs. An increasing amount of evidence indicates that miRNAs play important role in cancer pathogenesis, apoptosis, proliferation, and differentiation as oncogenes or tumor suppressors. Recently, miRNA-199a has been shown to be involved in many human cancers, although .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.20.5
- DOI:
- 10.4238/2013.August.20.5
Evaluation of candidate barcoding markers in Orinus (Poaceae)
Authors: X. Su, Y.P. Liu, Z. Chen and K.L. Chen
Orinus is an alpine endemic genus of Poaceae. Because of the imperfect specimens, high level of intraspecific morphological variability, and homoplasies of morphological characters, it is relatively difficult to delimitate species of Orinus by using morphology alone. To this end, the DNA barcoding has shown great potential in .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027714
- DOI:
- 10.4238/gmr.15027714
The kinin B2 receptor gene structure, product processing and expression in adult and fetal rats: evidence for gene evolution
Authors: C.E. Fran�§a, C.F. Vicari, A.M. Piza, E.A. Geroldo, M.L. Be�§ak, W. Be�§ak, R.C. Stocco and C.J. Lindsey
We examined the structure of the rat kinin B2 receptor gene (KB2r) and encoding messenger RNA (mRNA) processing. Differently from the closely related mouse and rabbit genes that have three exons and two introns, the rat gene purportedly consists of four exons and three introns. There are two purported gene products; one of the.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr681
- DOI:
- 10.4238/vol9-1gmr681