Rna
Temporal variations of Hsp60 and HSF-1 in primary rat myocardial cells in vitro under heat stress
Authors: R. Buriro, Y.J. Lv, I. Ali, S. Tang, Z.J. Liu, M. Zhang, A. Adem, J. Hartung and E.D. Bao
The mechanisms involved in sudden animal death due to acute heart failure during heat stress are not well understood. We examined the relationship between heat stress-induced variations of protective Hsp60 and expression of its regulatory factor, HSF-1, in heat-stressed primary myocardial cells of neonatal rats in vitro through cardiac enzyme detection, i.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.20.2
- DOI:
- 10.4238/2013.August.20.2
Meta-analysis of association of common variants in the KCNJ11-ABCC8 region with type 2 diabetes
Authors: L.J. Qin, Y. Lv and Q.Y. Huang
KCNJ11 (potassium inwardly rectifying channel, subfamily J, member 11) and ABCC8 (ATP-binding cassette, subfamily C (CFTR/MRP), member 8) have been studied for association with type 2 diabetes in various ethnic populations with contradictory results. We performed a comprehensive meta-analysis for KCNJ11 rs5219, rs5210, rs5215, and ABCC8 rs757110 to evalua.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.20.1
- DOI:
- 10.4238/2013.August.20.1
Agrobacterium-mediated transformation of the �²-subunit gene in 7S globulin protein in soybean using RNAi technology
Authors: J. Qu, S.Y. Liu, P.W. Wang, S.Y. Guan, Y.G. Fan, D. Yao, L. Zhang and J.L. Dai
The objective of this study was to use RNA interference (RNAi) to improve protein quality and decrease anti-nutritional effects in soybean. Agrobacterium tumefaciens-mediated transformation was conducted using RNAi and an expression vector containing the 7S globulin β-subunit gene. The BAR gene was used as the selective m.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027561
- DOI:
- 10.4238/gmr.15027561
Diversity of endophytic bacteria in Brazilian sugarcane
Authors: G.S. Magnani, C.M. Didonet, L.M. Cruz, C.F. Picheth, F.O. Pedrosa and E.M. Souza
Endophytic bacteria live inside plant tissues without causing disease. Studies of endophytes in sugarcane have focused on the isolation of diazotrophic bacteria. We examined the diversity of endophytic bacteria in the internal tissues of sugarcane stems and leaves, using molecular and biochemical methods. Potato-agar medium wa.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr703
- DOI:
- 10.4238/vol9-1gmr703
Monitoring tissue blood oxygen saturation in the internal jugular venous area using near infrared spectroscopy
Authors: Z.S. Ruan, T. Li, R.R. Ren, Y. Zhao, K. Li, Y.F. Mao, G. Shen and L. Jiang
Central venous blood oxygen saturation (ScvO2) is an important monitoring index of fluid resuscitation. However, monitoring of ScvO2 is not continuous and invasive. Near infrared spectroscopy (NIRS) is an optical technology for the noninvasive detection of hemodynamic changes, with advantages of being real-time, continuous, lo.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.31.23
- DOI:
- 10.4238/2015.March.31.23
Isolation and identification of new pollen-specific SFB genes in Japanese apricot (Prunus mume)
Authors: P.P. Wang, Z.H. Gao, Z.J. Ni, W.B. Zhuang and Z. Zhang
SFB, a candidate gene for the pollen S gene, has been identified in several species of Prunus (Rosaceae). We isolated 5 new SFB alleles from 6 Japanese apricot (Prunus mume) lines using a specific Prunus SFB primer pair (SFB-C1F and Pm-Vb), which was designed from conserved regions of Prunus SFB. The nucleotide sequences of these SFB genes were submitted .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.3.5
- DOI:
- 10.4238/2013.September.3.5
Genetic parameters for postweaning traits in Braunvieh cattle
Authors: D.C. Cucco, J.B.S. Ferraz, J.P. Eler, J.C.C. Balieiro, E.C. Mattos and L. Varona
Genetic parameters for traits related to postweaning growth in Braunvieh cattle, reared under tropical and sub-tropical conditions in Brazil, were studied. Weight traits were weight at 365 days of age (W365, N = 4055), at 450 days (W450, N = 3453), and at 550 days (W550, N = 1946), while weight gains were gain from weaning to .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr764
- DOI:
- 10.4238/vol9-1gmr764
Construction of recombinant adenoviruses carrying the optimal shRNA template against goat PTHrP and successful suppression of PTHrP expression in mammary epithelial cells
Authors: H.L. Zheng, X.M. Liu, Q. Yu, R.F. Xing, Z.Y. Yang and J. Luo
Parathyroid hormone-related protein (PTHrP) is a protein member of the parathyroid hormone family that regulates the dynamic balance between blood and bone calcium during lactation. However, the mechanism of its regulation is not very clear. In order to establish a framework for further functional studies of the PTHrP gene in goat mammary gland epithelial.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.4.2
- DOI:
- 10.4238/2013.September.4.2
Methylation regulation of liver-specific microRNA-122 expression and its effects on the proliferation and apoptosis of hepatocellular carcinoma cells
Authors: T.J. Xing, H.T. Xu, W.Q. Yu and D.F. Jiang
The regulation mechanism and significance of microRNA-122 (miRNA-122) expression are unclear. The aim of this study was to investigate the effects of DNA methylation on liver-specific miRNA-122 expression, cell proliferation, and apoptosis in hepatocellular carcinoma. Methylation of the miRNA-122 promoter region was detected through methylation sequencing.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.13.3
- DOI:
- 10.4238/2013.September.13.3
Identification of miRNAs and their targets in wheat (Triticum aestivum L.) by EST analysis
Authors: J. Han, M.L. Kong, H. Xie, Q.P. Sun, Z.J. Nan, Q.Z. Zhang and J.B. Pan
MicroRNAs (miRNAs) are a newly discovered class of noncoding small RNAs that regulate gene expression by directing target mRNA cleavage or translational inhibition. A large number of miRNAs have been identified in plants. Increasing evidence has shown that miRNAs play multiple roles in plant biological processes. So far, identification of miRNAs has been .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.19.11
- DOI:
- 10.4238/2013.September.19.11