Rna
Delimiting invasive Myriophyllum aquaticum in Kashmir Himalaya using a molecular phylogenetic approach
Authors: M.A. Shah, M.A. Ali, F.M. Al-Hemaid and Z.A. Reshi
Myriophyllum aquaticum (Vell.) Verdc. (family Haloragaceae) is one of the most invasive and destructive South American aquatic plant species and is present in a wide range of geographic regions, including the Kashmir Himalaya. Confusion regarding the taxonomic delimitation of M. aquaticum in the Himalayan region impedes effective and tar.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.233539
- DOI:
- 10.4238/2014.September.12.233539
A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression
Authors: H. Li, Q. Jiang, Z.H. Ju, J.M. Huang, X.G. Wang, C.H. Yang, Y. Sun, G.W. Yang, J.F. Zhong and C.F. Wang
The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is a newly identified protein that is specifically expressed in testis tissue and participates in spermatogenesis. In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain react.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027723
- DOI:
- 10.4238/gmr.15027723
Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease
Authors: N.F. Rodrigues, J. K�¤stle, T.J.D. Coutinho, A.T. Amorim, G.B. Campos, V.M. Santos, L.M. Marques, J. Timenetsky and S.T. de Farias1
The microbial community of the reproductive apparatus, when known, can provide information about the health of the host. Metagenomics has been used to characterize and obtain genetic information about microbial communities in various environments and can relate certain diseases with changes in this community composition. In this study, samples of vaginal .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.12.4
- DOI:
- 10.4238/2015.June.12.4
Transcript analysis of a goat mesenteric lymph node by deep next-generation sequencing
Authors: G.X. E, Y.J. Zhao, R.S. Na and Y.F. Huang
Deep RNA sequencing (RNA-seq) provides a practical and inexpensive alternative for exploring genomic data in non-model organisms. The functional annotation of non-model mammalian genomes, such as that of goats, is still poor compared to that of humans and mice. In the current study, we performed a whole transcriptome analysis .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028262
- DOI:
- 10.4238/gmr.15028262
Evaluation of the semen swim-up method for bovine sperm RNA extraction
Authors: C.M. Han, R. Chen, T. Li, X.L. Chen, Y.F. Zheng, M.T. Ma and Q.H. Gao
Isolation of high-quality RNA is important for assessing sperm gene expression, and semen purification methods may affect the integrity of the isolated RNA. This study evaluated the effectiveness of the sperm swim-up method for seminal RNA isolation. Frozen semen samples in straws from three bulls of proven fertility were puri.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027713
- DOI:
- 10.4238/gmr.15027713
Effect of siRNA-induced silencing of cellular prion protein on tyrosine hydroxylase expression in the substantia nigra of a rat model of Parkinson�s disease
Authors: X. Wang, H.A. Yang, X.N. Wang and Y.F. Du
The most significant pathological feature of Parkinson’s disease (PD) is the progressive degeneration of dopaminergic (DA) neurons in the substantia nigra. Currently, available treatments for PD cannot prevent the loss of DA neurons. Tyrosine hydroxylase (TH) expressed in substantia nigra neurons catalyzes the conversion.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027406
- DOI:
- 10.4238/gmr.15027406
Drosha, DGCR8, and Dicer mRNAs are down-regulated in human cells infected with dengue virus 4, and play a role in viral pathogenesis
Authors: S.M.M. Casseb, D.B. Simith, K.F.L. Melo, M.H. Mendon�§a, A.C.M. Santos, V.L. Carvalho, A.C.R. Cruz and P.F.C. Vasconcelos
Dengue virus (DENV) and its four serotypes (DENV1-4) belong to the Flavivirus genus of the Flaviviridae family. DENV infection is a life-threatening disease, which results in up to 20,000 deaths each year. Viruses have been shown to encode trans-regulatory small RNAs, or microRNAs (miRNAs), which bind to messenger RNA and nega.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027891
- DOI:
- 10.4238/gmr.15027891
Phylogenetic relationships among Octopodidae species in coastal waters of China inferred from two mitochondrial DNA gene sequences
Authors: Z.M. L�¼, W.T. Cui, L.Q. Liu, H.M. Li and C.W. Wu
Octopus in the family Octopodidae (Mollusca: Cephalopoda) has been generally recognized as a “catch-all” genus. The monophyly of octopus species in China’s coastal waters has not yet been studied. In this paper, we inferred the phylogeny of 11 octopus species (family Octopodidae) in China’s coastal waters using nucleotide sequences.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.19.7
- DOI:
- 10.4238/2013.September.19.7
Increased expression of the lncRNA BANCR and its prognostic significance in human osteosarcoma
Authors: Z.Q. Peng, R.B. Lu, D.M. Xiao and Z.M. Xiao
Long noncoding RNAs (lncRNAs) play important roles in the formation and progression of many types of human malignancies. The aim of our study was to investigate the expression and biological functions of the lncRNA BRAF-activated noncoding RNA (BANCR) in human osteosarcoma. BANCR expression was quantified by real-time PCR in h.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017480
- DOI:
- 10.4238/gmr.15017480
miRQuest: integration of tools on a Web server for microRNA research
Authors: R.R. Aguiar, L.A. Ambrosio, G. Sep�ºlveda-Hermosilla, V. Maracaja-Coutinho and A.R. Paschoal
This report describes the miRQuest - a novel middleware available in a Web server that allows the end user to do the miRNA research in a user-friendly way. It is known that there are many prediction tools for microRNA (miRNA) identification that use different programming languages and methods to realize this task. It is diffic.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016861
- DOI:
- 10.4238/gmr.15016861