Rna
Pivotal role of microRNA-9 in osteosarcoma tumorigenesis and tumor progression
Authors: X.J. Qi, J.F. Wang, G.D. Wang, Q. Xu and H.L. Sun
MicroRNA-9 (miR-9) has a well-established role in various tumors; the clinical significance and potential mechanism of miR-9 in human osteosarcoma (OS) has not been elucidated. The aim of this study was to investigate the mechanism and role of miR-9 expression in osteosarcoma cells. miR-9 expression in the OS cell line MG-63 and OS tissues was compared to.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017318
- DOI:
- 10.4238/gmr.15017318
MicroRNA analysis in model species based on evolutionary rates
Authors: X.F. Mao and Y.C. Cao
MicroRNAs (miRNAs) are major post-transcriptional regulators of gene expression. In an attempt to gain insights into miRNAs at the macroevolutionary level, we performed a systematic analysis of miRNAs in six model organisms based on their evolutionary rates. First, we calculated their miRNA evolutionary rates, and found that they did not correlate with th.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017216
- DOI:
- 10.4238/gmr.15017216
Novel splice variants of the bovine PCK1 gene
Authors: Z.B. Zhang, W. Zhang, R.L. Li, J.B. Li, J.F. Zhong, Z.S. Zhao and J.M. Huang
Phosphoenolpyruvate carboxykinase 1 (PCK1), also named PEPCK-C, is a multiple-function gene that is involved in gluconeogenesis, glyceroneogenesis, reproduction, female fertility, and development of obesity and diabetes. How its many functions are regulated was largely unknown. Therefore, we investigated mRNA expression and possible splice variants of PCK.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.4
- DOI:
- 10.4238/2013.September.27.4
Identification of sequence-related amplified polymorphism markers linked to the red leaf trait in ornamental kale (Brassica oleracea L. var. acephala)
Authors: Y.S. Wang, Z.Y. Liu, Y.F. Li, Y. Zhang, X.F. Yang and H. Feng
Artistic diversiform leaf color is an important agronomic trait that affects the market value of ornamental kale. In the present study, genetic analysis showed that a single-dominant gene, Re (red leaf), determines the red leaf trait in ornamental kale. An F2 population consisting of 500 individuals from the cross of a red leaf double-haploid line â.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.3
- DOI:
- 10.4238/2013.April.2.3
Complementary DNA cloning, sequence analysis, and tissue transcription profile of a novel U2AF2 gene from the Chinese Banna mini-pig inbred line
Authors: S.Y. Wang, J.L. Huo, Y.W. Miao, W.M. Cheng and Y.Z. Zeng
U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an important gene for pre-messenger RNA splicing in higher eukaryotes. In this study, the Banna mini-pig inbred line (BMI) U2AF2 coding sequence (CDS) was cloned, sequenced, and characterized. The U2AF2 complete CDS was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) technique.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.9
- DOI:
- 10.4238/2013.April.2.9
Effects of siRNA-mediated silencing of Sal-like 4 expression on proliferation and apoptosis of prostate cancer C4-2 cells
Authors: K.F. Liu and Y.X. Shan
The aim of this study was to evaluate the effects of small interfering RNA (siRNA)-inhibited expression of the Sal-like 4 (SALL4) gene on the proliferation, colony formation, and apoptosis of prostate cancer C4-2 cells. C4-2 cells were cultured and divided into a si-SALL4 group, a negative control siRNA group, and a blank cont.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027885
- DOI:
- 10.4238/gmr.15027885
microRNAs in avian influenza virus H9N2-infected and non-infected chicken embryo fibroblasts
Authors: X. Peng, Q.S. Gao, L. Zhou, Z.H. Chen, S. Lu, H.J. Huang, C.Y. Zhan and M. Xiang
There is limited information about microRNAs (miRNAs) in H9N2 subtype influenza virus-infected chicken cells or tissues. In this study, 10,487,469 and 13,119,795 reads were obtained from infected and non-infected chicken embryo fibroblasts, respectively. Seven hundred and thirty-six and 1004 miRNAs, including mature miRNAs and precursors, were obtained fr.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.17
- DOI:
- 10.4238/2015.August.7.17
Computational identification and characterization of conserved miRNAs and their target genes in beet (Beta vulgaris)
Authors: J.L. Li, J. Cui and D.Y. Cheng
Highly conserved endogenous non-coding microRNAs (miRNAs) play important roles in plants and animals by silencing genes via destruction or blocking of translation of homologous mRNA. Sugar beet, Beta vulgaris, is one of the most important sugar crops in China, with properties that include wide adaptability and strong tolerance to salinity and impoverished.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.19
- DOI:
- 10.4238/2015.August.7.19
Detection of Piwi-interacting RNAs based on sequence features
Authors: Y.J. Liu, J.Y. Zhang, A.M. Li, Z.W. Liu, Y.Y. Zhang and X.H. Sun
Piwi-interacting RNAs (piRNAs) are a class of small non-coding RNAs. Distinguishing piRNAs from other non-coding RNAs is important because of their important role in the physiological regulation of spermatogenesis, genome protection from transposons, and regulation of mRNAs and long non-coding RNAs. Few computational studies h.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028638
- DOI:
- 10.4238/gmr.15028638
Decreased microRNA-143 expression and its tumor suppressive function in human oral squamous cell carcinoma
Authors: Z.Y. Ni, F.O. Lin, D.F. Liu and J. Xiao
MicroRNA-143 serves as a tumor suppressor in many human malignancies. However, its involvement in oral squamous cell carcinoma (OSCC) is still unclear. In this study, we investigated the effects of miR-143 in OSCC tumorigenesis and development. Using real-time quantitative reverse transcription-polymerase chain reaction, we detected miR-143 expression in .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.2
- DOI:
- 10.4238/2015.June.26.2