Rna
Expression of miR-98 in myocarditis and its influence on transcription of the FAS/FASL gene pair
Authors: B.Y. Zhang, Z. Zhao and Z. Jin
Myocarditis is a common cardiovascular disease and frequently occurs in children and teenagers. It is believed to be caused by both endogenous and exogenous factors, among which FAS/FASL gene pair-induced cell apoptosis is a major mechanism of myocardial cell injury. A previous study has detected low expression of microRNA (mi.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027627
- DOI:
- 10.4238/gmr.15027627
A structured sparse regression method for estimating isoform expression level from multi-sample RNA-seq data
Authors: L. Zhang and X.J. Liu
With the rapid development of next-generation high-throughput sequencing technology, RNA-seq has become a standard and important technique for transcriptome analysis. For multi-sample RNA-seq data, the existing expression estimation methods usually deal with each single-RNA-seq sample, and ignore that the read distributions ar.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027670
- DOI:
- 10.4238/gmr.15027670
Comparison of two methods for RNA extraction from the nucleus pulposus of intervertebral discs
Authors: M.F. Gan, H.L. Yang, J.L. Qian, C.S. Wu, C.X. Yuan, X.F. Li and J. Zou
RNA extraction from the nucleus pulposus of intervertebral discs has been extensively used in orthopedic studies. We compared two methods for extracting RNA from the nucleus pulposus: liquid nitrogen grinding and enzyme digestion. The RNA was detected by agarose gel electrophoresis, and the purity was evaluated by absorbance r.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027738
- DOI:
- 10.4238/gmr.15027738
Microarray analysis of microRNA deregulation and angiogenesis-related proteins in endometriosis
Authors: R.Q. Yang, H. Teng, X.H. Xu, S.Y. Liu, Y.H. Wang, F.J. Guo and X.J. Liu
We examined the aberrant microRNA (miRNA) expression profile responsible for the changes in angiogenesis observed in endometriotic lesions. This study revealed characteristic miRNA expression profiles associated with endometriosis in endometrial tissue and endometriotic lesions from the same patient, and their correlation with.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027826
- DOI:
- 10.4238/gmr.15027826
A phytotoxin Solanapyrone-A downregulates calcium-dependent protein kinase activity in potato
Authors: A. Hassan, N. Hatsugai and M.M. Shah
We previously demonstrated that alternaric acid, a host-specific toxin produced by the plant pathogenic fungus Alternaria solani, in the presence of Ca2+ and Mg2+, stimulated in vitro phosphorylation of His-tagged calcium-dependent protein kinase 2 from potato cultivar Rishiri (RiCDPK2). Herein, we report that Solanapyrone-A (SpA), a non-host-specific tox.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.13.7
- DOI:
- 10.4238/2013.May.13.7
Role of the CKIP1 gene in proliferation and apoptosis of the human lung cancer cell line H1299
Authors: G.M. Chen,R.F. Ding,Y.D. Tan,X.B. Pan,G.M. Jiang,J.F. He,S.H. Lin,C. Liu and Y. Jia
Casein kinase 2 interacting protein 1 (CKIP1) is a specific interacting protein of the casein kinase 2 (CK2) α subunit, and, by binding CK2 and other proteins, functions as an adaptor to regulate a series of cellular functions. Previous studies suggested that CKIP1 might play an important role in regulating oncogenic activities. However, few studies.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.15
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.15
Analysis and clinical significance of microRNA-499 expression levels in serum of patients with acute myocardial infarction
Authors: C.H. Zhao,G.-C. Cheng,R.L. He,Y. Hong,Q.L. Wan,Z.-Z. Wang and Z.-Y. Pan
The aim of this study was to investigate the expression level of microRNA-499 and its clinical significance in serum of patients with acute myocardial infarction (AMI). We recruited 59 patients with AMI and 60 healthy individuals undergoing physical examination in our hospital during the same period as controls. Peripheral blood was drawn in the morning o.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.17
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.17
Effect of siRNA targeting HER2/neu on the proliferation and viability of prostate cancer PC-3M cells
Authors: C.Y. Liu, P.C. Xu, D.G. Chen, X.H. Fan, M.Q. Li, X. Yang and Y.P. Xu
The aim of this study was to investigate the effect of a small interfering RNA (siRNA) targeting human epidermal growth factor receptor 2 (HER2/neu) on the proliferation and viability of prostate cancer PC-3M cells. Chemically synthesized siRNA targeting HER2/neu was transfected into PC-3M cells by using liposomes, and cells transfected with empty liposom.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.14
- DOI:
- 10.4238/2015.December.16.14
Telomere-associated factor expression in replicative senescence of human embryonic lung fibroblasts
Authors: H. Du, L. Yang, X.-Y. Xu, L. Hai, Y.-Q. Han and Y.-X. Shi
The objective of this study was to find the key regulatory molecules in the cell senescence process through observing the expression of telomere-associated factor during the normal cell replicative senescence process. Based on the established cell replicative senescence model, reverse transcription-polymerase chain reaction and western blot analyses were .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.7
- DOI:
- 10.4238/2015.August.10.7
MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG
Authors: J.J. Li,W.H. Chan,W.Y. Leung,Y. Wang and C.S. Xu
Rat liver regeneration (RLR) induced by partial hepatectomy involves cell proliferation regulated by numerous factors, including microRNAs (miRNAs). miRNA high-throughput sequencing has been established and used to analyze miRNA expression profiles. This study showed that 39 miRNAs were related to RLR through the analysis of miRNA high-throughput sequenci.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.30
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.30