Rna
An optimized preparation method to obtain high-quality RNA from dry sunflower seeds
Authors: X.B. Ma and J. Yang
In an attempt to isolate high-quality, intact total RNA from sunflower (Helianthus annuus) seeds for investigation of the molecular mechanisms of mutations, we tested various procedures, using kits, including RNAiso Plus, RNAiso Plus+RNAiso-mate for Plant Tissue, Trizol, and the Qi method, but no high-quality total RNA of high.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr979
- DOI:
- 10.4238/vol10-1gmr979
MicroRNA profiling in cutaneous wounds of diabetic rats
Authors: Y.-F. Liu, M. Ding, D.-W. Liu, Y. Liu, Y.-G. Mao and Y. Peng
Despite years of effort, current therapies for diabetic wounds are still not fully efficacious. Emerging evidence has suggested that microRNAs (miRNAs) play key roles in multiple physiological and pathological processes in eukaryotes, and could potentially be powerful therapeutic tools. This study investigated the differential expression profiling of miRN.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.24
- DOI:
- 10.4238/2015.August.14.24
Silencing FKBP38 gene by siRNA induces activation of mTOR signaling in goat fetal fibroblasts
Authors: Y.T. Fu, X. Zheng, Q. He, X.Y. Jia, Z.X. Guo, R.Y. Yao, Y.F. Wang and Z.G. Wang
FKBP38 (also known as FKBP8) is a unique member of the FK506-binding protein (FKBP) family, and its role is controversial because it acts as an upstream regulator of the mTOR signaling pathway, which controls cell growth, proliferation, and differentiation. This study aimed to explore the role of FKBP38 in the activation of mTOR signaling in Cashmere goat.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.30
- DOI:
- 10.4238/2015.August.14.30
Are Epichlo�« endophytes specific to Elymus grass hosts?
Authors: H. Song, Q.Y. Song, X.Z. Li and Z.B. Nan
Epichloë endophytes are widely distributed mutualists of cool-season grasses and can protect their hosts against biotic and abiotic stresses. Previous studies have shown that Epichloë endophytes are specific to their grass hosts in tall Festuca and Lolium species. However, no systematic analysis exists of host specificity of asexual Epichloë.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.17
- DOI:
- 10.4238/2015.December.21.17
N-ethylmaleimide-sensitive factor siRNA improves cardiac function following myocardial infarction in rats
Authors: Y. Zhou , Y. Liu, S.X. Yang and Z. Wang
This study examined the effects of N-ethylmaleimide-sensitive factor (NSF) small interfering RNA (siRNA) on cardiac function following myocardial infarction (MI) in rats. Thirty-six adult Sprague Dawley rats were randomly divided into three equivalent groups. An acute MI model was established by ligating the anterior descending branch of the left coronary.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.11
- DOI:
- 10.4238/2015.August.14.11
HPV infection-associated anogenital cyto-colpo-histological findings and molecular typing in HIV-positive women
Authors: F.K. Tso, C.L.L. Rodrigues, J.E. Levi, M.G. Mattosinho de Castro Ferraz, N.M.G. Speck and J.C.L. Ribalta
HIV and human papillomavirus (HPV) coinfection is increasing, especially in the anal canal (AC) and cervico-vaginal regions.We identified anal epithelium abnormalities related to high-risk HPV (HR-HPV) lesions in the lower genital tracts (LGTs) of HIV-positive women, described the HPV genotypes identified, and assessed the expression of E6/E7 oncogenes in.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.36
- DOI:
- 10.4238/2015.December.21.36
In silico analysis of the 16S rRNA gene of endophytic bacteria, isolated from the aerial parts and seeds of important agricultural crops
Authors: C. Bredow, J.L. Azevedo, J.A. Pamphile, C.A. Mangolin and S.A. Rhoden
Because of human population growth, increased food production and alternatives to conventional methods of biocontrol and development of plants such as the use of endophytic bacteria and fungi are required. One of the methods used to study microorganism diversity is sequencing of the 16S rRNA gene, which has several advantages, including universality, size.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.3
- DOI:
- 10.4238/2015.August.19.3
Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein
Authors: T. Ocampo Ocampo, S.M. Gabriel Peralta, N. Bacheller, S. Uiterwaal, A. Knapp, A. Hennen, D.L. Ochoa-Martinez and H. Garcia-Ruiz
In addition to regulating gene expression, RNA silencing is an essential antiviral defense system in plants. Triggered by double-stranded RNA, silencing results in degradation or translational repression of target transcripts. Viruses are inducers and targets of RNA silencing. To condition susceptibility, most plant viruses en.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028625
- DOI:
- 10.4238/gmr.15028625
Characterization of microRNAs from goat (Capra hircus) by Solexa deep-sequencing technology
Authors: Y.H. Ling, J.P. Ding, X.D. Zhang, L.J. Wang, Y.H. Zhang, Y.S. Li, Z.J. Zhang and X.R. Zhang
MicroRNAs (miRNAs) are an important class of small noncoding RNAs that are highly conserved in plants and animals. Many miRNAs are known to mediate a myriad of cell processes, including proliferation and differentiation, via the regulation of some transcription and signaling factors, which are closely related to muscle development and disease. In this stu.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.13.4
- DOI:
- 10.4238/2013.June.13.4
Identification of SNPs within the PRLR gene and effects on maternal behavior in sheep
Authors: L.P. Wang, R.Q. Geng, X.N. Zhang and W. Sun
The prolactin receptor gene (PRLR) plays an essential role in maternal behavior. The aim of the study was to detect PRLR mutations in exon 10, using a polymerase chain reaction-single stranded conformation polymorphism method, and to determine the association between mutations in this region with maternal behavior traits in Chinese Hu sheep. Polymorphisms.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.26
- DOI:
- 10.4238/2015.December.21.26