Rna
Identification of long non-coding RNA involved in osteogenic differentiation from mesenchymal stem cells using RNA-Seq data
Authors: W.Q. Song, W.Q. Gu, Y.B. Qian, X. Ma, Y.J. Mao and W.J. Liu
The aim of this study was to identify long non-coding RNA (lncRNA) associated with osteogenic differentiation from mesenchymal stem cells (MSCs) using high-throughput RNA sequencing (RNA-Seq) data. RNA-Seq dataset was obtained from the European Bioinformatics Institute (accession No. PRJEB4496), including two replicates each for immortalized mesenchymal s.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.14
- DOI:
- 10.4238/2015.December.23.14
Analysis of the expression of HMGB-1, CXCL16, miRNA-30a, and TGF-�²1 in primary nephritic syndrome patients and its significance
Authors: W.J. Wang, X.Q. Qu, X.M. Yu, W. Lv and H.Y. Yu
We investigated the expression levels of high-mobility group box protein 1 (HMGB-1), CXC chemokine ligand 16 (CXCL16), microRNA (miRNA)-30a and transforming growth factor b1 (TGF-b1) in primary nephritic syndrome (PNS) patients and the clinical significance of this expression. A total of 56 patients with PNS were included in the PNS group, while 50 health.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.17
- DOI:
- 10.4238/2015.August.19.17
Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense
Authors: S.B. Jin, H.T. Fu, S.F. Jiang, Y.W. Xiong, H. Qiao, W.Y. Zhang, Y.S. Gong and Y. Wu
The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China. The androgenic gland produces hormones that play crucial roles in the differentiation of crustaceans to the male sex. MicroRNA (miRNA) post-transcriptionally regulates many protein-coding genes, influencing important biological and metabolic processes. Howeve.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.27
- DOI:
- 10.4238/2015.December.23.27
Prospective uses of recombinant Lactococcus lactis expressing both listeriolysin O and mutated internalin A from Listeria monocytogenes as a tool for DNA vaccination
Authors: M.S.P. De Azevedo, C. Santos Rocha, V.B. Pereira, A.F. De Oliveira Junior, C.S. De Sousa, V. Azevedo, J.G. LeBlanc, J.M. Chatel and A. Miyoshi
In this study, Lactococcus lactis was engineered to express mutated internalin A on its surface and to secrete large amounts of listeriolysin O (LLO) in order to improve its potential as a vehicle for DNA vaccination. Western blotting experiments demonstrated that the bacterium expressed LLO in both the cytoplasmic and extracellular compartments, with hig.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.36
- DOI:
- 10.4238/2015.December.23.36
Mitochondrial tRNA mutations may be infrequent in hepatocellular carcinoma patients
Authors: G. Li, Y.X. Duan, X.B. Zhang and F. Wu
Mitochondrial DNA mutations have been shown to play important roles in the pathogenesis of hepatocellular carcinoma (HCC). In particular, genes encoding mitochondrial tRNA (mt-tRNA) are hotspots for pathogenic mutations associated with HCC. Recently, an increasing number of studies have reported the involvement of such mutatio.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027665
- DOI:
- 10.4238/gmr.15027665
Effect of Bifidobacterium on the mRNA expression levels of TRAF6, GSK-3�², and microRNA-146a in LPS-stimulated rat intestinal epithelial cells
Authors: W. Zhou, Y. Yuan, J. Li, W.M. Yuan, L.G. Huang and S.W. Zheng
We investigated the effect of inactivated Bifidobacterium on the mRNA expression of TRAF6, GSK-3β, and microRNA-146a in lipopolysaccharide (LPS)-stimulated rat small intestinal epithelial cells (IEC-6s). IEC-6s were randomly divided into an LPS group, a culture supernatant group, and an inactivated bacteria group. After stimulation with LPS for 5 h, .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.11
- DOI:
- 10.4238/2015.August.21.11
Analysis of the relationship between peripheral blood T lymphocyte subsets and HCV RNA levels in patients with chronic hepatitis C.
Authors: Y. Huang, M.J. Zheng and Y.H. Xu
We investigated the relationship between peripheral blood T lymphocyte subsets and hepatitis C virus (HCV) RNA levels in patients with hepatitis C. Samples from 69 chronic hepatitis C (CHC) patients and 20 healthy controls were analyzed using quantitative polymerase chain reaction (PCR) to detect HCV RNA and flow cytometry to determine the expression leve.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.12
- DOI:
- 10.4238/2015.August.21.12
Effects of stathmin 1 silencing by siRNA on sensitivity of esophageal cancer cells Eca-109 to paclitaxel
Authors: H.W. Zhu, D. Jiang, Z.Y. Xie, M.H. Zhou, D.Y. Sun and Y.G. Zhao
We investigated the effects of stathmin 1 (STMN1) silencing by small interfering (siRNA) on the sensitivity of esophageal cancer cells Eca-109 to paclitaxel. STMN1 siRNA was transiently transfected into Eca-109 cells. The effects of transfection were detected by quantitative polymerase chain reaction and western blotting. The effects of STMN1 silencing by.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.18
- DOI:
- 10.4238/2015.December.28.18
miRNA-146a induces vascular smooth muscle cell apoptosis in a rat model of coronary heart disease via NF-�ºB pathway
Authors: Z.W. Wu, Y.F. Liu, S. Wang and B. Li
The aim of this study was to investigate the role of miRNA-146a in modulating the function of vascular smooth muscle cells in a rat model of coronary heart disease. Vascular smooth muscle cells were isolated and cultured from the rat coronary heart disease model and normal rats (controls). miRNA-146a levels were measured in vascular smooth muscle cells ob.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.19
- DOI:
- 10.4238/2015.December.28.19
Effect of DNase treatment on RNA extraction from preimplantation murine embryos
Authors: J. Norhazlin, M.N.K. Nor-Ashikin, B.P. Hoh, S.H. Sheikh Abdul Kadir, S. Norita, M. Mohd-Fazirul, W.J. Wan-Hafizah, D. Razif, M.H. Rajikin and B. Abdullah
The quality of RNA is crucial when performing microarray experiments. This is particularly important when dealing with preimplantation embryos, from which a minimum yield of RNA of good quality can be produced. We report the optimization of several RNA extraction methods applied to preimplantation embryos at different stages of development. The quality of.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.28.1
- DOI:
- 10.4238/2015.August.28.1