Rna
Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep
Authors: M. Yang, Y.J. Yue, T.T. Guo, J.L. Han, J.B. Liu, J. Guo, X.P. Sun, R.L. Feng, Y.Y. Wu, C.F. Wang, L.P. Wang and B.H. Yang
Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.7
- DOI:
- 10.4238/2014.April.8.7
Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L.
Authors: C.R. Li1,2*, D.D. Liang1,2*, R.F. Xu1,2*, H. Li2,3, Y.P. Zhang2, R.Y. Qin2, L. Li1,2, P.C. Wei2,3 and J.B. Yang2
Low temperature is a major environmental stress in rice cultivating and production. The alternative oxidase 1 (AOX1) gene is potentially important for genetic engineering to increase cold adaptation. However, previous studies related to this effect have mostly focused on the dicot plants Arabidopsis and tobacco, whereas functional research on rice is limi.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.4
- DOI:
- 10.4238/2013.November.11.4
Analysis of cell cycle shortening and developmental interruption in Dictyostelium discoideum allC RNAi mutants
Authors: D.M. Xue, L.S. Hou, S.R. Zhang and N.X. Chen
Dictyostelium discoideum allC RNAi mutant cells are motile and aggregate together, but do not undergo further morphological development. The relatively quick growth rate of allC RNAi mutants compared to wild-type D. discoideum results in a shortened mutant cell cycle. However, at present, little is known about the mechanism un.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.6
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.6
Universal primers to amplify the complete mitochondrial 12S rRNA gene in marine fish species
Authors: X.X. Jin, S.L. Zhao, R.X. Wang
A pair of new universal 12S mitochondrial rRNA gene primers was designed through multiple alignment analysis of the mitochondrial tRNAPhe and the 5’ region of 16S mitochondrial rRNA genes of different kinds of fishes. The primers were successfully used to amplify an expected product fragment of about 1.2 kb from various marine fish species, and the .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.6
- DOI:
- 10.4238/2013.October.15.6
Interleukin-1�² +3954 polymorphisms and risk of external apical root resorption in orthodontic treatment: A meta-analysis
Authors: F.-L. Wu, L.-Y. Wang, Y.-Q Huang, W.-B. Guo, C.-D. Liu and S.-G. Li
The purpose of this meta-analysis was to determine whether genetic variants of the interleukin-1β[+3954 C>T (rs1143634)] (IL-1β +3954 C>T) gene polymorphisms were associated with orthodontic external apical root resorption (EARR). A meta-analysis was carried out using data entered into the PubMed and Embase electronic databases before Octo.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.6
- DOI:
- 10.4238/2013.October.18.6
A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization
Authors: Y.Q. Zhu1,2, W.J. Wu1,2, H.W. Xiao1,2, H.B. Chen1,2, Y. Zheng1,2, Y.J. Zhang2, H.X.Wang2 and L.Q. Huang1,2
A recently developed revolutionary approach to transcriptomics, RNA-Seq, and suppression subtractive hybridization are powerful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that.. Read More»
- Genet. Mol. Res. 12(4):
Molecular phylogenetic relationships among members of the family Phytolaccaceae sensu lato inferred from internal transcribed spacer sequences of nuclear ribosomal DNA
Authors: J. Lee, S.Y. Kim, S.H. Park and M.A. Ali
The phylogeny of a phylogenetically poorly known family, Phytolaccaceae sensu lato (s.l.), was constructed for resolving conflicts concerning taxonomic delimitations. Cladistic analyses were made based on 44 sequences of the internal transcribed spacer of nuclear ribosomal DNA from 11 families (Aizoaceae, Basellaceae, Didiereaceae, Molluginaceae, Nyctagin.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.15
- DOI:
- 10.4238/2013.February.28.15
Culture-independent analysis of endophytic bacterial communities associated with Brazilian sugarcane
Authors: G.S. Magnani1, L.M. Cruz1, H. Weber2, J.C. Bespalhok2, E. Daros2, V. Baura1, M.G. Yates1, R.A. Monteiro1, H. Faoro1, F.O. Pedrosa1 and E.M. Souza1
Sugarcane is an economically important culture in Brazil. Endophytic bacteria live inside plants, and can provide many benefits to the plant host. We analyzed the bacterial diversity of sugarcane cultivar RB-72454 by cultivation-independent techniques. Total DNA from sugarcane stems from a commercial plantation located in Paraná State was extracted.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.3
- DOI:
- 10.4238/2013.October.15.3
MicroRNA expression profiling studies on bronchopulmonary dysplasia: a systematic review and meta-analysis
Authors: Y. Yang, J. Qiu, Q. Kan, X.-G. Zhou and X.-Y. Zhou
Over the past several years, several microRNA (miRNA) expression profiling studies have been carried out on bronchopulmonary dysplasia (BPD) in mammalian lung tissues. The most effective way to identify these important miRNAs is to systematically search for similar signatures identified in multiple independent studies. Accordingly, a meta-analysis was con.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.30.4
- DOI:
- 10.4238/2013.October.30.4
Study on the treatment of the p15 gene combined with Bcr-abl-specific siRNA and STI571 for chronic myeloid leukemia
Authors: W. Wang, Y. Du, N.-N. Li, F.-F. Lv and G.-Q. Lin
The aim of this study was to investigate the effect of the p15 gene combined with Bcr-abl-specific siRNA and STI571 on the proliferation, cell cycle and apoptosis of K562 chronic myeloid leukemia cells. Using the gene sequence results, we amplified the p15 gene from normal peripheral blood by RT-PCR, and constructed a p15-pcDN.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.30.4
- DOI:
- http://dx.doi.org/10.4238/2014.May.30.4