Authors: B. Cao, T. Ji, B. Zhou, J. Zou, G.Q. Jiao
MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides in length, which play important roles in regulating gene expression post-transcriptionally. Several computational methods and algorithms have been developed to predict miRNA targets. In this study, we described a method that can be used to integrate m.. Read More»
Authors: S.H. Habib, H.M. Saud and H. Kausar
Oil palm tissues are rich in polyphenols, polysaccharides and secondary metabolites; these can co-precipitate with RNA, causing problems for downstream applications. We compared two different methods (one conventional and a kit-based method - Easy-BlueTM Total RNA Extraction Kit) to isolate total RNA from leaves, roots and shoot apical meristems of tissue.. Read More»
Authors: S. Wang, Y.Z. Liu, D.D. Qin, C.C. Zou, Y.R. Sheng A.L. Huang and H. Tang
Hepatitis B virus S protein (HBs) plays an important role in hepatocellular carcinoma progression. However, to date, no direct and effective methods exist to research the function of HBs. Here, we combined the technology of RNA interference with recombinant adenovirus, constructed a recombinant adenovirus-expressing small hair.. Read More»
Authors: L. Li, Q. Gao, X. Mao and Y. Cao
MicroRNA (miRNA) plays important roles in cell differentiation, proliferation, growth, mobility, and apoptosis. An accurate list of precise target genes is necessary in order to fully understand the importance of miRNAs in animal development and disease. Several computational methods have been proposed for miRNA target-gene id.. Read More»
Authors: X. Cui, F. Liu, J.Q. Wang, W.J. Zhang, J.Y. Wang, K. Liu, S.Y. Cui, J. Zhang and R.R. Xu
This study was primarily undertaken to test the hypothesis that mitochondrial DNA (mtDNA) mutations may be associated with aplastic anemia (AA). We analyzed mtDNA sequences from 15 patients with AA. The samples were obtained from bone marrow, and patients’ oral epithelial cells were collected for normal tissue comparison. Total DNA was amplified by .. Read More»
Authors: R. Wei and J.-P. Zang
We investigated the effects of kinase-domain insert containing receptor (KDR) gene silencing on the proliferation of A549 cells and their sensitivity to docetaxel. After designing and synthesizing the KDR siRNA sequence, the sequence was transfected into A549 cells using Lipofectamine 2000. The expression of KDR.. Read More»
Authors: A. Hassan, T. Okuta, M. Kato, N. Hatsugai, Y. Sano, T. Ishimori, K. Okazaki, M.A. Doullah and M.M. Shah
Calcium-dependent protein kinases (CDPK) are an essential component of plant defense mechanisms against pathogens. We investigated the effect of alternaric acid, a host-specific toxin produced by the plant fungal pathogen Alternaria solani (Pleosporaceae), on a putative plasma membrane and cytosolic kinase RiCDPK2 of potato (Solanum tuberosum) and on hype.. Read More»
Authors: Y.R. Gu, Y. Liang, J.J. Gong, K. Zeng, Z.Q. Li, Y.F. Lei, Z.P. He and X.B. Lv
Determination of an optimal set/number of internal control microRNA (miRNA) genes is a critical, but often undervalued, detail of quantitative gene expression analysis. No validated internal genes for miRNA quantitative PCR (q-PCR) in pig milk were available. We compared the expression stability of six porcine internal control miRNA genes in pig milk from.. Read More»
Authors: W.L. Zhu and Y.H. Liu
We investigated the effects of kinase insert domain receptor (KDR) gene silencing on the proliferation of A549 cells and their sensitivity to erlotinib. A KDR small interfering RNA (siRNA) sequence was designed and synthesized; then, it was transfected into A549 cells using LipofectamineTM 2000. KDR mRNA and protein expression after KDR gene si.. Read More»
Authors: L. Yan, Y. Yao, L.H. Wang, M.L. Wang and X.H. Fu
The expression of CK19, LUNX, and KS1/4 mRNA biomarkers was detected in the peripheral blood of non-small cell lung cancer (NSCLC) patients to investigate the feasibility of indicating lung cancer micrometastases. Micrometastases were identified in the peripheral blood of 32 NSCLC patients, 15 benign pulmonar.. Read More»