Rna
Differential expression of microRNAs may regulate pollen development in Brassica oleracea
Authors: J.H. Song, J. Yang*, F. Pan and B. Jin
MicroRNAs (miRNAs) are a class of non-coding endogenous negative regulators that regulate gene expression at both the transcriptional and post-transcriptional levels. However, little is known about the expression characteristics of miRNAs during pollen development in Brassica oleracea. In this study, five known and th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.10
- DOI:
- 10.4238/2015.November.24.10
Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells
Authors: Q.D. Jiang, H.P. Li, F.J. Liu, X.J. Wang, Y.J. Guo, L.F. Wang, W.F. Lu, H.J. Li, X.P. Li and Y.Y. Wang
This study aimed to compare the effects of lipopolysaccharide (LPS) on stearoyl-coenzyme A desaturase (SCD) gene expression in mouse primary hepatic cells. To obtain sufficient total RNA, primary hepatic cells were plated on 6-cm diameter-type collagen 1-coated dishes (1 x 106 cells per dish). The test was divided into 6 group.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.24.12
- DOI:
- 10.4238/2014.January.24.12
Genetic variability among the chloroplast genomes of sugarcane (Saccharum spp) and its wild progenitor species Saccharum spontaneum L.
Authors: J.-R. Zhu, H. Zhou, Y.-B. Pan and X. Lu
A striking characteristic of modern sugarcane is that all sugarcane cultivars (Saccharum spp) share a common cytoplasm from S. officinarum. To explore the potential value of S. spontaneum cytoplasm, new Saccharum hybrids with an S. spontaneum cytoplasm were developed at the United States Department of Agriculture-Agricultural .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.24.3
- DOI:
- 10.4238/2014.January.24.3
Characterization of ESTs from black locust for gene discovery and marker development
Authors: J.X. Wang, C. Lu, C.Q. Yuan, B.B. Cui, Q.D. Qiu, P. Sun, R.Y. Hu, D.C. Wu, Y.H. Sun and Y. Li
Black locust (Robinia pseudoacacia L.) is an ecologically and economically important species. However, it has relatively underdeveloped genomic resources, and this limits gene discovery and marker-assisted selective breeding. In the present study, we obtained large-scale transcriptome data using a next-generation sequencing pl.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.12
- DOI:
- 10.4238/2015.October.19.12
Isolation and expression analysis of four HD-ZIP III family genes targeted by microRNA166 in peach
Authors: C.H. Zhang, B.B. Zhang, R.J. Ma, M.L. Yu, S.L. Guo and L. Guo
MicroRNA166 (miR166) is known to have highly conserved targets that encode proteins of the class III homeodomain-leucine zipper (HD-ZIP III) family, in a broad range of plant species. To further understand the relationship between HD-ZIP III genes and miR166, four HD-ZIP III family genes (PpHB14, PpHB15, PpHB8, and PpREV) were isolated from peach (Prunus .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.37
- DOI:
- 10.4238/2015.October.29.37
Identification of conserved microRNAs in peripheral blood from giant panda: expression of mammary gland-related microRNAs during late pregnancy and early lactation
Authors: C.D. Wang1,2*, K. Long1*, L. Jin1, S. Huang2, D.H. Li2, X.P. Ma3, M. Wei2, Y. Gu4, J.D. Ma1 and H. Zhang1,2
The giant panda (Ailuropoda melanoleuca) is one of the world’s most endangered mammals, and it has evolved several unusual biological and behavioral traits. During puberty, pregnancy, lactation, and involution, the mammary gland undergoes profound morphological and functional changes. A large number of microRNAs (miRNAs) have been identified to be i.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.5
- DOI:
- 10.4238/2015.November.13.5
Eosinophil cationic protein mRNA expression in children with bronchial asthma
Authors: H.Y. Yu1, X.Y. Li2, Z.F. Cai1, L. Li3, X.Z. Shi4, H.X. Song5 and X.J. Liu1
Studies have shown that eosinophils are closely related to pathogenesis of bronchial asthma. Eosinophils release eosinophil cationic protein (ECP), which plays an important role in infection and allergic reactions. Serum ECP mRNA expression in children with bronchial asthma has not been adequately investigated. We analyzed serum ECP mRNA expression in 63 .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.11
- DOI:
- 10.4238/2015.November.13.11
Molecular characterization, expression, polymorphism of NR5A2 and its relationship with litter size in Hu sheep
Authors: Y.X. Li, J. Zhang, Y. Qian, C.H. Meng, H.L. Wang, X.J. Tao, S. Zhong, S.X. Cao and Q.F. Li
NR5A2 has been implicated in processes as diverse as steroidogenesis, cellular proliferation, ovarian follicular development, ovulation, and fertility in mammals. However, data about the relationship between NR5A2 and prolificacy in mammals are lacking. In the present study, we identified and characterized NR5A2 of Hu sheep, a.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.20
- DOI:
- 10.4238/2015.October.19.20
Real-time PCR evaluation of cell-free DNA subjected to various storage and shipping conditions
Authors: Q. Wang, Y. Cai, P. Brady and J.R. Vermeesch
In this study, we attempted to explore the factors affecting the yield of cell-free fetal DNA (cffDNA) obtained from maternal blood samples, including the use of different types of collection tubes, the interval between sample processing, and sample shipping under extreme weather conditions. Blood samples were drawn into K3EDT.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.23
- DOI:
- 10.4238/2015.October.19.23
Segment-specific targeting via RNA interference mediates down-regulation of OPN expression in hepatocellular carcinoma cells
Authors: F. Lin1*, C.M. Huang2*, J. Cao1, Z.H. Pei1, W.L. Gu1, S.F. Fan1, K.P. Li1 and C.M. Lin1
Osteopontin (OPN) plays an important role in the metastasis and recurrence of tumors after resection of hepatocellular carcinoma (HCC). In this study, the down-regulation effect on OPN expression in HCC cells of RNA interference (RNAi) molecules designed to target different segments of OPN was investigated to identify a more effective site for OPN knockdo.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.6
- DOI:
- 10.4238/2015.November.18.6