Rna
PPAR�± signal pathway gene expression is associated with fatty acid content in yak and cattle longissimus dorsi muscle
Authors: W. Qin, C.N. Liang, X. Guo, M. Chu, J. Pei, P.J. Bao, X.Y. Wu, T.K. Li and P. Yan
ntramuscular fatty acid (FA) is related to meat qualities such as juiciness, tenderness, palatability, and shear force. PPARα plays an important role in lipid metabolism in the liver and skeletal muscle. This study investigated FA composition in yaks and cattle, in order to ascertain whether a correlation between PPARα signal pathway genes as .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.9
- DOI:
- 10.4238/2015.November.18.9
Effect of Bcl-2-siRNA on proliferation and apoptosis of pediatric acute B lymphoblastic leukemia (A-BLL) cells
Authors: W.B. Meng, J.P. Liu, X.W. Wang and L.H. E
This study analyzed the effect of small interfering RNA specific for the Bcl-2 gene (siRNA Bcl-2) on the proliferation and chemotherapeutic sensitivity of pediatric A-BLL cells. Marrow samples were obtained from sixty newly-diagnosed A-BLL pediatric patients. The Bcl-2 mRNA expression in these samples was quantified by real ti.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.16.9
- DOI:
- 10.4238/2015.October.16.9
Analysis of ITS1 sequences and genetic relationships between populations of ridgetail white prawn, Exopalaemon carinicauda, in the East China Sea
Authors: X.Z. Zhang, X.Q. Cheng, Y.X. Yu, H. Shen and X.H. Wan
Internal transcribed spacer 1 (ITS1) sequences from wild-type Exopalaemon carinicauda (N = 124) from the East China Sea were amplified and sequenced. Sequences were polymorphic and ranged from 388 to 583 bp in length. The average content of GC in sequences was significantly higher than that of AT. Altogether, 604 mutant sites .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.20
- DOI:
- 10.4238/2015.October.9.20
Cloning and sequence analysis of the coding sequence of �²-actin cDNA from the Chinese alligator and suitable internal reference primers from the �²-actin gene
Authors: H.N. Zhu, S.Z. Zhang, Y.K. Zhou, C.L. Wang and X.B. Wu
β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.4
- DOI:
- 10.4238/2015.October.9.4
Identification of RNA editing sites in cotton (Gossypium hirsutum) chloroplasts and editing events that affect secondary and three-dimensional protein structures
Authors: Y. Jiang, S.L. Fan, M.Z. Song, J.N. Yu and S.X. Yu
RNA editing can alter individual nucleotides in primary transcripts, which can cause the amino acids encoded by edited RNA to deviate from the ones predicted from the DNA template. We investigated RNA editing sites of protein-coding genes from the chloroplast genome of cotton. Fifty-four editing sites were identified in 27 tra.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.4
- DOI:
- 10.4238/2012.April.19.4
Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR
Authors: C. Wang, C.-L. Zhao, L. Liu, R. Wu and X.-L. Zhang
Doppel is a homologue of cellular prion protein (PrP)-like protein (PrPC). Different tissue samples were collected from the central nervous system plus four regions of lymphoid system, eleven regions of digestive tract and two reproductive organs from four ARR/ARQ and four ARH/ARQ sheep, genotypes of the PrP gene. Total RNA wa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.3
- DOI:
- 10.4238/2015.October.9.3
Assessment of correlation between pre-miRNA-1757 polymorphism and chicken performance traits
Authors: H. Li, K.R. Jiang, S.H. Wang, X.J. Liu, X.T. Kang, R.R. Jiang,Z.J. Li and G.R. Sun
Single nucleotide polymorphism in microRNAs (miRNA) may influence their target gene selection and regulation efficiency, leading to animal phenotypic variation. The aim of this study was to evaluate the possible effect of single nucleotide polymorphisms in the miRNA-1757 gene precursor region (pre-mir-1757) on economic-related.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.7
- DOI:
- 10.4238/2015.October.9.7
Regulation of the expression of zinc finger protein genes by microRNAs enriched within acute lymphoblastic leukemia-derived microvesicles
Authors: L. Lu, X.M. Chen, H.M. Tao, W. Xiong, S.H. Jie and H.Y. Li
Microvesicles (MVs) are submicrometric membrane fragments that can “engulf” cytoplasmic contents such as microRNAs (miRNAs) from their cellular origin. The study of miRNAs carried within MVs might provide insights into the roles that miRNAs play in the underlying pathophysiologic processes of acute lymphoblastic le.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.2
- DOI:
- 10.4238/2015.October.5.2
Inhibition of gap junctions relieves the hepatotoxicity of TNF-�±
Authors: Z.Y. Chen, R. Wang, F. Huang, D.D. Yuan and S.R. Li
The aim of this study was to observe the influence of gap junction (GJ) functional changes on the hepatotoxicity of TNF-α. Three different methods were employed to study functional effects of the GJ inhibition: 1) pretreatment with a GJ inhibitor; 2) inoculation of cells at high and low densities; and 3) inhibition of th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.3
- DOI:
- 10.4238/2015.October.5.3
Integrity of prokaryotic mRNA isolated from complex samples for in vivo bacterial transcriptome analysis
Authors: A.B. Ferreira-Machado, M.C.R. Freitas, G.R.Q. Saji, A.B. Rezende, P.E. Almeida, D.E. Cesar, J.A. Resende1, M.F. Nic�³las2, V.L. Silva and C.G. Diniz
Even though several in vitro studies have focused on bacterial biology, the extent of such knowledge is not complete when considering an actual infection. As culture-independent microbiology methods such as high-throughput sequencing became available, important aspects of host-bacterium interactions will be elucidated. Based on microbiological relevance, .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.40
- DOI:
- 10.4238/2015.November.18.40