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De novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress
Authors: A.A. Belesini, F.M.S. Carvalho, B.R. Telles, G.M. de Castro, P.F. Giachetto, J.S. Vantini, S.D. Carlin, J.O. Cazetta, D.G. Pinheiro and M.I.T. Ferro
Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16028845
- DOI:
- 10.4238/gmr16028845
Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements
Authors: N. Marini, C.B. Bevilacqua, M.V. B?¼ttow, M.C.B. Raseira and S. Bonow
Selecting and validating reference genes are the first steps in studying gene expression by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The present study aimed to evaluate the stability of five reference genes for the purpose of normalization when studying gene expression in various cultivars of Pru.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029666
- DOI:
- 10.4238/gmr16029666
Genetic divergence among corn hybrids and combining ability for agronomic and bromatological traits of silage
Authors: E. Gralak, M.V. Faria, A.S.T. Figueiredo, D.A. Rizzardi, M. Neumann, M.C. Mendes, C.A. Scapim and S. Galbeiro
We assessed the impact of genetic divergence and the ability to combine corn hybrids used for the production of silage on the agronomic and bromatological traits of silage quality. We evaluated 18 corn hybrids used as genitors in a circulant diallel scheme in which each genitor hybrid participated in 9 hybrid combinations, and.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029643
- DOI:
- 10.4238/gmr16029643
SETD5 gene variant associated with mild intellectual disability - a case report
Authors: E. Stur, L.A. Soares and I.D. Louro
The recent advent of exome sequencing has allowed for the identification of pathogenic gene variants responsible for a variety of diseases that were previously clinically diagnosed, with no underlying molecular etiology. Among these conditions, intellectual disability is a prevalent heterogeneous condition, presenting itself i.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029615
- DOI:
- 10.4238/gmr16029615
Expressed sequence tags related to nitrogen metabolism in maize inoculated with Azospirillum brasilense
Authors: L. Pereira-Defilippi, E.M. Pereira, F.M. Silva and G.V. Moro
The relative quantitative real-time expression of two expressed sequence tags (ESTs) codifying for key enzymes in nitrogen metabolism in maize, nitrate reductase (ZmNR), and glutamine synthetase (ZmGln1-3) was performed for genotypes inoculated with Azospirillum brasilense. Two commercial single-cross hybrids (AG7098 and 2B707.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029682
- DOI:
- 10.4238/gmr16029682
Restoration over time and sustainability of Schinus terebinthifolius Raddi
Authors: S.V. ?lvares-Carvalho, R. Silva-Mann, I.B. Gois, M.F.V. Melo, A.S. Oliveira, R.A. Ferreira and L.J. Gomes
The success of recovery programs on degraded areas is dependent on the genetic material to be used, which should present heterozygosity and genetic diversity in native and recovered populations. This study was carried out to evaluate the model efficiency to enable the recovery of a degraded area of the Lower São Francis.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029669
- DOI:
- 10.4238/gmr16029669
Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture
Authors: M.J.S. Carvalho, E.J. Oliveira, A.S. Souza, J.S. Pereira, M.S.A.S. Diamantino and S.A.S. Oliveira
This study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease .. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029556
- DOI:
- 10.4238/gmr16029556
ERCC1 mRNA expression is associated with the clinical outcome of non-small cell lung cancer treated with platinum-based chemotherapy
Authors: H. Zhang1, J. Li2, Y. Zhang2, M. Sun3, P. Zhao1, G. Zhang2, C. Jin2, L. Sun1, M. He1, B. Wang2 and X. Zhang2
We conducted a prospective study to analyze the expression of the excision repair cross-complementing group 1 (ERCC1) and ribonucleotide reductase subunit M1 (RRM1) genes in 297 Chinese patients with advanced non-small cell lung cancer (NSCLC). The goal of this study was to evaluate these genes as potential biomarkers for prediction of tumor response and .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.16
- DOI:
- 10.4238/2014.December.4.16
ERCC1 mRNA expression is associated with the clinical outcome of non-small cell lung cancer treated with platinum-based chemotherapy
Authors: H. Zhang1, J. Li2, Y. Zhang2, M. Sun3, P. Zhao1, G. Zhang2, C. Jin2, L. Sun1, M. He1, B. Wang2 and X. Zhang2
We conducted a prospective study to analyze the expression of the excision repair cross-complementing group 1 (ERCC1) and ribonucleotide reductase subunit M1 (RRM1) genes in 297 Chinese patients with advanced non-small cell lung cancer (NSCLC). The goal of this study was to evaluate these genes as potential biomarkers for prediction of tumor response and .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.16
- DOI:
- 10.4238/2014.December.4.16
Molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain
Authors: J.H. Hu1,2*, F.Y. Zhang1*, K.J. Jiang1, Y.B. Fang1,2, J. Wang1,2, M. Zhao1,2, Z.G. Qiao1 and L.B. Ma1
The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.19
- DOI:
- 10.4238/2014.December.4.19