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Comparison of methods for high quantity and quality genomic DNA extraction from raw cow milk
Authors: T. Usman, Y. Yu, C. Liu, Z. Fan and Y. Wang
Isolation of sufficient quantities of high quality DNA is a prerequisite for molecular studies. Milk somatic cells can be used; however, inhibitors such as fats and proteins make milk a difficult medium for extracting large amounts of quality DNA. We optimized, evaluated and compared three methods, Modified Nucleospin Blood Ki.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.29.10
- DOI:
- 10.4238/2014.April.29.10
Effect of secondary lymphoid tissue chemokine suppression on experimental ulcerative colitis in mice
Authors: H. Zhang, X. Zhang, X. Ding, W. Cao, L. Qu and G. Zhou
The secondary lymphoid tissue chemokine (CCL21) is closely associated with lymphoid homing and anti-tumor immune responses. CCL21 also has a chemotactic effect on intestinal lymphocytes. This study mainly focused on CCL21 expression in experimental ulcerative colitis and on the effects of CCL21 suppression on this disease in m.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.12
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.12
Sequence variation in the Toxoplasma gondii eIF4A gene among strains from different hosts and geographical locations
Authors: J. Chen, S.F. Fang, D.H. Zhou, Z.Y. Li, G.H. Liu and X.Q. Zhu
Toxoplasma gondii is an opportunistic protozoan parasite that infects a wide range of animals, including humans. The T. gondii eukaryotic translation initiation factor 4A (eIF4A) protein is expressed in the tachyzoite, but its expression is markedly downregulated in the bradyzoite, and it is therefore considered to be associat.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.14
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.14
A novel nonsense mutation in the sedlin gene (SEDL) causes severe spondyloepiphyseal dysplasia tarda in a five-generation Chinese pedigree
Authors: X.Y. Xia, J. Yu, W.W. Li, N. Li, Q.Y. Wu , X. Zhou, Y.X. Cui and X.J. Li
Spondyloepiphyseal dysplasia tarda (SEDT) is an X-linked recessive osteochondrodysplasia characterized by disproportionately short stature and degenerative joint disease. The objective of this study was to describe a novel nonsense mutation in the sedlin gene (SEDL) causing severe SEDT in a large Chinese pedigree. The clinical.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.15
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.15
Relationship between serum neopterin levels and coronary heart disease
Authors: Z.Y. Liu and Y.D. Li
The relationship between serum neopterin levels and coronary heart disease (CHD) was investigated. Eighty-six CHD patients were divided into an acute myocardial infarction (AMI) group (N = 21), an unstable angina pectoris (UAP) group (N = 35), and a stable angina pectoris (SAP) group (N = 30), based on coronary angiography (CAG), 30 subjects without CHD s.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.8
- DOI:
- 10.4238/2013.October.7.8
Impact of forage clipping treatments on performance of winter wheat
Authors: S.A. Jadoon1, H. Ullah1, F. Mohammad2, I.H. Khalil2, M. Alam1,D. Shahwar1, M.F.A. Malik1 and Y. Jamal1
Farmers in northern parts of Pakistan face severe shortage of green forage for their livestock during the harsh winter season. Winter wheat has the potential to be used as a dual-purpose crop for forage plus grain production in these areas. Ten elite winter wheat lines from Oklahoma State University were evaluated at Hazara Research Station Abbottabad und.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.November.7.3
- DOI:
- 10.4238/2013.November.7.3
Expressions of Src homology 2 domain-containing phosphatase and its clinical significance in laryngeal carcinoma
Authors: L.B. Dong1, G.Q. Li2, Z.H. Tian2, Z.M. Wang2 and K. Xu2
We investigated the expression of Src homology 2 domain-containing phosphatase (SHP-2) in laryngeal carcinoma and its clinical significance. Expression of SHP-2 was detected by immunohistochemical staining in normal mucosal tissues and various grades of laryngeal carcinoma. We looked for possible correlations between expression of SHP-2 in laryngeal carci.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.6
- DOI:
- 10.4238/2013.October.7.6
Expression of tyrosine hydroxylase and growth-associated protein 43 in aging atrial fibrillation patients of Xinjiang Uygur and Han nationality
Authors: Y.D. Li1, J.X. Li1, B.-P. Tang1, T.Y. Gan1, G.J. Xu1, X.H. Zhou1, H. Li2,X. Guo2, A. Mahemuti1, Q. Sun3, Y.Y. Zhang1 and J. Wang1
The aim of this study was to explore the changes in gene and protein expressions of tyrosine hydroxylase (TH) and growth-associated protein 43 (GAP43) in aging atrial fibrillation patients of Xinjiang Uygur and Han nationality, and the significance of the changes. Real-time polymerase chain reaction and Western blot analysis were used to detect gene and p.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.10
- DOI:
- 10.4238/2013.October.30.10
Molecular characteristic and expression analysis of collagenolytic serine protease from the Chinese mitten crab Eriocheir sinensis with defense response to Vibrio anguillarum challenge
Authors: Q.Z. Yang, Z.J. Yang, Y. Zhang, X.L. Li and W. Zhang
A novel collagenolytic serine protease (CLSP) was cloned from the hemocytes of the Chinese mitten crab Eriocheir sinensis (Es-CLSP). The full-length cDNA of Es-CLSP contains 990 nucleotides. It encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. It contains the conserved catalytic triad (H.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.1
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.1
Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus
Authors: L.N. Sun1,2, H.S. Yang1, M.Y. Chen3 and D.X. Xu1,2
Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein con.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.7
- DOI:
- 10.4238/2013.November.7.7