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Development of 1047 insertion-deletion markers for rice genetic studies and breeding
Authors: Y.X. Zeng*, Z.H. Wen*, L.Y. Ma, Z.J. Ji, X.M. Li and C.D. Yang
In this study, a total of 1047 insertion-deletion (InDel) primer pairs distributed across the rice genome were developed and experimentally validated. The primer pairs were designed based on the InDel length polymorphisms between 93-11 (Oryza sativa ssp indica cv.) and Nipponbare (Oryza sativa ssp japonica cv.), aiming for utilization between indica and j.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.7
- DOI:
- 10.4238/2013.October.30.7
Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)
Authors: W. Zhao1,2, L. Chen1, F. Zhang1, P. Wu1, E. Li1 and J. Qin3
We identified the cDNA sequence of cathepsin L (CatL) in Macrobrachium nipponense, designated as MnCatL, for the first time. The MnCatL cDNA, isolated from the ovary, was 1710 bp in length, containing a 31-bp 5'-untranslated region, a 650-bp 3'-untranslated region, and an open reading frame of 1029 bp, encoding a 342-amino aci.. Read More»
- Genet. Mol. Res. 13(4):
- 2013.October.30.6
- DOI:
- 10.4238/2013.October.30.6
Effect of variation of ABCB1 and GSTP1 on osteosarcoma survival after chemotherapy
Authors: J.Z. Li, Z.Q. Tian, S.N. Jiang and T. Feng
We conducted a comprehensive study to investigate the role of genes involved in metabolic and transport pathways in response to chemotherapy and clinical outcome of osteosarcoma patients. Genotyping of seven gene polymorphisms was performed on a 384-well plate format on the Sequenom MassARRAY platform in 162 patients with oste.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.25.3
- DOI:
- 10.4238/2014.April.25.3
Clinical application of carbon nanoparticle lymph node tracer in the VI region lymph node dissection of differentiated thyroid cancer
Authors: S.P. Sun, Y. Zhang, Z.Q. Cui, Q. Chen, W. Zhang, C.X. Zhou, P.P. Xie and B.G. Liu
The application and clinical significance of carbon nanoparticle lymph tracer in the VI region (central region) lymph node dissection of differentiated thyroid cancer was investigated. Eighty patients with differentiated thyroid cancer were equally divided into the carbon nanoparticle-marked group (ipsilateral thyroid injectio.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.30.4
- DOI:
- http://dx.doi.org/10.4238/2014.April.30.4
Optimization of a biolistic transformation system for transfer of antifreeze gene KN2 and the bar herbicide resistance gene in common wheat
Authors: L. Cai, D.F. Sun and G.L. Sun
We studied the effects of different media for callus induction and differentiation, and pre-culture period of immature wheat embryo culture on biolistic transformation efficiency for including antifreeze gene KN2 and bar conferring resistance to the herbicide bialaphos. The percentage of plantlets generated from induction and .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.30.8
- DOI:
- http://dx.doi.org/10.4238/2014.April.30.8
Association of the CYP1A1 MspI and TNF�±-308 polymorphisms with chronic obstructive pulmonary disease in Inner Mongolia
Authors: L. Yang, F. Li2*, M. Yan and X. Su
Chronic obstructive pulmonary disease (COPD) is a progressive lung disease characterized by persistent airflow limitation. Smoking, occupational exposures, air pollution, and genetics are all risk factors. In the present study, we detected the cytochrome P4501A1 gene (CYP1A1) MspI polymorphism and the tumor necrosis factor alp.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.25.6
- DOI:
- 10.4238/2014.April.25.6
Ginsenoside Rh2 promotes nonamyloidgenic cleavage of amyloid precursor protein via a cholesterol-dependent pathway
Authors: J. Qiu, W. Li, S.H. Feng, M. Wang and Z.Y. He
Ginsenoside Rh2 (Rh2) is a ginseng derivative used in Chinese traditional medicine. We investigated whether Rh2 can help prevent Alzheimer’s disease symptoms and examined underlying mechanisms. We injected Rh2 into tg2576 Alzheimer’s disease model mice and looked for behavioral improvement and senile plaque reducti.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.2
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.2
Evaluation of powdery mildew-resistance of grape germplasm and rapid amplified polymorphic DNA markers associated with the resistant trait in Chinese wild Vitis
Authors: J. Zhang, Y. Zhang, H. Yu1, and Y. Wang
The resistance of wild Vitis germplasm, including Chinese and American wild Vitis and Vitis vinifera cultivars, to powdery mildew (Uncinula necator Burr.) was evaluated for two consecutive years under natural conditions. Most of the Chinese and North American species displayed a resistant phenotype, whereas all of the European.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.3
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.3
Proteins differentially expressed by Shiga toxin-producing Escherichia coli strain M03 due to the biliar salt sodium deoxycholate
Authors: C.B.A. Ribeiro1, M.G. Sobral1, C.L. Tanaka1, C.B. Dallagassa1, G. Picheth1, F.G.M. Rego1, D. Alberton1, L.F. Huergo2, F.O. Pedrosa2, E.M. Souza2 and C.M.T. Fadel-Picheth1
Shiga toxin-producing Escherichia coli (STEC) can cause conditions ranging from diarrhea to potentially fatal hemolytic uremic syndrome. Enteropathogen adaptation to the intestinal environment is necessary for the development of infection, and response to bile is an essential characteristic. We evaluated the response of STEC strain M03 to the bile salt so.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.24.1
- DOI:
- 10.4238/2013.October.24.1
A Medicago truncatula H+-pyrophosphatase gene, MtVP1, improves sucrose accumulation and anthocyanin biosynthesis in potato (Solanum tuberosum L.)
Authors: J.W. Wang, H.Q. Wang, W.W. Xiang and T.Y. Chai
We recently cloned MtVP1, a type I vacuolar-type H+-translocating inorganic pyrophosphatase from Medicago truncatula. In the present study, we investigated the cellular location and the function of this H+-PPase in Arabidopsis and potato (Solanum tuberosum L.). An MtVP1::enhanced green fluorescent protein fusion was constructe.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.4
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.4