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Plant Genetics   Research Article

Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp) in Malaysia

Authors: M.A. Intan Sakinah, I.V. Suzianti and Z. Latiffah

Anthracnose caused by Colletotrichum species is a common postharvest disease of banana fruit. We investigated and identified Colletotrichum species associated with anthracnose in several local banana cultivars based on morphological characteristics and sequencing of ITS regions and of the β-tubulin gene. Thirty-eight Coll.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.9.5
DOI:
http://dx.doi.org/10.4238/2014.May.9.5
Animal Genetics   Research Article

Expression analysis of differentially expressed miRNAs in male and female chicken embryos

Authors: Y.P. Feng, J.F. Chen, P. Huang, X. Wang, J. Wang, X.L. Peng and Y.Z. Gong

MicroRNAs (miRNAs) are small non-coding RNA molecules that play key roles in the regulation of development processes of many tissues and organs at the post-transcriptional level. However, little is known about how they affect chicken gonadal development. We examined the expression of four miRNAs (miR-218, -200b, -196, and -206.. Read More»

Genet. Mol. Res. 13(2):
2014.April.17.2
DOI:
10.4238/2014.April.17.2
Human Genetics   Research Article

Genetic association of catechol-O-methyltransferase val(158)met polymorphism in Saudi schizophrenia patients

Authors: S. Al-Asmary, S. Kadasah, M. Arfin, M. Tariq and A. Al-Asmari

Schizophrenia is a complex neuropsychiatric disorder strongly associated with dopamine dysregulation. Catechol-O-methyl-transferase (COMT) is a candidate gene for schizophrenia that encodes an enzyme involved in the metabolic inactivation of dopamine. The COMT Val158Met polymorphism has been associated with schizophrenia and h.. Read More»

Genet. Mol. Res. 13(2):
2014.April.17.4
DOI:
10.4238/2014.April.17.4
Plant Genetics   Research Article

Proteomic response of Moniliophthora perniciosa exposed to pathogenesis-related protein-10 from Theobroma cacao

Authors: F.A.C. Silva1, C.P. Pirovani1, S. Menezes1, C. Pungartnik2, A.S. Santiago1, M.G.C. Costa1, F. Micheli3 and A.S. Gesteira4

TcPR-10, a member of the pathogenesis-related protein 10 family, was identified in EST library of interactions between Theobroma cacao and Moniliophthora perniciosa. TcPR-10 has been shown to have antifungal and ribonuclease activities in vitro. This study aimed to identify proteins that are differentially expressed in M. perniciosa in response to TcPR-10.. Read More»

Genet. Mol. Res. 12(4):
2013.October.22.5
DOI:
10.4238/2013.October.22.5
Plant Genetics   Research Article

Molecular detection of QTL controlling plant height components in a doubled haploid barley population

Authors: X.F. Ren, D.F. Sun, W.B. Dong, G.L. Sun and C.D. Li

Yield losses caused by lodging in barley can be partially controlled by reducing plant height. In order to understand dwarfing mechanisms and efficiently use new dwarf germplasms for a breeding program, it is important to identify QTL of plant height components. QTL analysis was performed for seven plant height component traits using a DH population of 12.. Read More»

Genet. Mol. Res. 13(2):
2014.April.17.5
DOI:
10.4238/2014.April.17.5
Microbial Genetics   Research Article

Comparison of the sensitivity of strains of Salmonella enterica serovar Typhimurium in the detection of mutagenicity induced by nitroarenes

Authors: C.R. Rainho, S.M. CorrĂ?ÂȘa, J.L. Mazzei, C.A.F. Aiub and I. Felzenszwalb

The use of strains of Salmonella enterica serovar Typhimurium with different metabolic capacities can indicate the class or classes of compounds present in an environmental sample and enable the diagnosis of the mutagenic activity of these pollutants adsorbed on particulate matter (PM) in the air. In the present study, the sen.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.9.9
DOI:
http://dx.doi.org/10.4238/2014.May.9.9
Human Genetics   Research Article

Rapid method for DNA extraction from the honey bee Apis mellifera and the parasitic bee mite Varroa destructor using lysis buffer and proteinase K

Authors: M.R.C. Issa1, V.L.C. Figueiredo2, D. De Jong1, C.H. Sakamoto1 and Z.L.P. Sim�”es2

We developed a rapid method for extraction of DNA from honey bees, Apis mellifera, and from the parasitic bee mite, Varroa destructor. The advantages include fast processing and low toxicity of the substances that are utilized. We used lysis buffer with nonionic detergents to lyse cell walls and proteinase K to digest proteins. We tested whole thorax, tho.. Read More»

Genet. Mol. Res. 12(4):
2013.October.22.4
DOI:
10.4238/2013.October.22.4
Human Genetics   Research Article

Clinical features of diabetes mellitus cases complicated by Burkholderia pseudomallei septicemia

Authors: H.B. Quan, T.Y. Li, Y.Y. Gao and D.X. Chen

The aim of this study was to analyze the clinical characteristics of diabetes mellitus patients with Burkholderia pseudomallei septicemia and evaluate strategies of diagnosis and treatment. The clinical characteristics, diagnosis, treatment, and prognosis of 39 diabetes mellitus patients with B. pseudomallei septicemia were re.. Read More»

Genet. Mol. Res. 13(2):
2014.April.17.7
DOI:
10.4238/2014.April.17.7
Plant Genetics   Research Article

Molecular cloning and expression analysis of the ethylene insensitive3 (EIN3) gene in cucumber (Cucumis sativus)

Authors: B.B. Bie, J.S. Pan, H.L. He, X.Q. Yang, J.L. Zhao and R. Cai

The plant gaseous hormone ethylene regulates many aspects of plant growth, development, and responses to the environment. Ethylene insensitive3 (EIN3) is a key transcription factor involved in the ethylene signal transduction pathway. To gain a better understanding of this particular pathway in cucumber, the full-length cDNA encoding EIN3 (designated as C.. Read More»

Genet. Mol. Res. 12(4):
2013.October.7.4
DOI:
10.4238/2013.October.7.4
Human Genetics   Research Article

Association of GSTTI and GSTM1 variants with acute myeloid leukemia risk

Authors: Y.M. Zi, S. Wu, D. Ma, C. Yang, M. Yang, Y. Huang and S.J. Yang

We aimed to investigate the relationships between polymorphisms of the glutathione S-transferases (GSTs) GSTM1, GSTTI, and GSTP1 and the risk of developing acute myeloid leukemia (AML). A total of 206 AML cases and 231 controls were collected for our study. The genotyping of GSTs (GSTM1, GSTTI, and GSTP1) was based upon the du.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.9.11
DOI:
http://dx.doi.org/10.4238/2014.May.9.11