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Molecular authentication of multi-species honeysuckle tablets
Authors: C. Jiang*, Y. Yuan*, M. Chen and L. Huang
Authenticating multi-species original raw materials in commercial formulations is difficult. Jin Yin Hua and Shan Yin Hua, both classified as raw honeysuckle materials in the Chinese Pharmacopoeia, are used in various medicines. Differentiating one variety from another is difficult based on chemical analysis. We developed molecular authentication of multi.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.2
- DOI:
- 10.4238/2013.October.22.2
Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance
Authors: J. Wang, Y. Liu, W.H. Dong, Y.J. Huo, X.G. Huang, S.L. Wu and W.B. Bao
The transporter associated with antigen processing (TAP) transports peptides from the cytosol into the endoplasmic reticulum for subsequent loading onto the major histocompatibility complex (MHC) class I molecules. This study showed the dynamic changes in the TAP1 expression level in newborn to weaning piglets. Tissue expressi.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.12
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.12
DNA elimination in embryogenic development of Pennisetum glaucum x Pennisetum purpureum (Poaceae) hybrids
Authors: J.D. Nunes1, A.L.S. Azevedo1, A.V. Pereira1, C.M.P. Paula2, J.M.S. Campos3, F.J.S. L�©do1 and V.B. Santos4
Interspecific hybridization between Napier grass (Pennisetum purpureum), which is widely grown in Brazil for cattle forage, and pearl millet (Pennisetum glaucum) has been used as a breeding strategy for the development of improved cultivars. However, the hybrid between these two species is sterile due to its triploid condition (2n = 3x = 21 chromosomes), .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.1
- DOI:
- 10.4238/2013.October.22.1
Association of genetic polymorphisms of SAA1 (rs12218) with myocardial infarction in a Chinese population
Authors: J.W. Wang, H.Q. Wang, W.W. Xiang and T.Y. Chai
We recently cloned MtVP1, a type I vacuolar-type H+-translocating inorganic pyrophosphatase from Medicago truncatula. In the present study, we investigated the cellular location and the function of this H+-PPase in Arabidopsis and potato (Solanum tuberosum L.). An MtVP1::enhanced green fluorescent protein fusion was constructe.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.4
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.4
Marker-assisted selection in breeding silkworm strains with high silk production and resistance to the densonucleosis virus
Authors: C.X. Hou1, P.J. Sun1, X.J. Guo1,2, Y.P. Huang3 and M.W. Li1,2
In the silkworm (Bombyx mori), resistance to the Zhenjiang (China) strain of the densonucleosis virus (DNV-Z) is controlled by the recessive gene nsd-Z (non-susceptible to DNV-Z), which is linked to 7 simple-sequence repeat markers. Marker-assisted evaluation and selection of DNV-Z-resistant silkworms were used for predicting DNV-resistance in backcrossed.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.3
- DOI:
- 10.4238/2013.October.7.3
Low-frequency ultrasound induces apoptosis of rat aortic smooth muscle cells (A7r5) via the intrinsic apoptotic pathway
Authors: B. Zhang, H.S. Zhou, Q. Cheng, L. Lei and B. Hu
Ultrasound, a non-invasive therapy method, is a potential tool for medical applications, but its biological effects on vascular smooth muscle cells (VSMCs) have not been characterized. The aim of this study was to explore the effect and possible apoptotic mechanism of VSMCs that were induced by low-frequency ultrasound (LFU). .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.10
- DOI:
- 10.4238/2014.April.17.10
A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat
Authors: W.L. Du, J. Wang, L.M. Wang, Y.H. Pang, J. Wu, J.X. Zhao, Q.H. Yang and X.H. Chen
In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequence-characterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search t.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.16
- DOI:
- 10.4238/2013.October.18.16
Expression profiling analysis of hypoxic pulmonary disease
Authors: L. Zhou, L.M. Wang, H.M. Song, Y.Q. Shen, W.J. Xu, J.H. Xu, Y. Liu, W.W. Yan and J.F. Jiang
Exposure of humans to low levels of environmental oxygen results in alveolar hypoxia and normally causes chronic pulmonary hypertension and morphological alterations of precapillary pulmonary vessels. In this study, the microarray dataset GSE11341 was used to identify potential differentially expressed genes related with human lung microvascular endotheli.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.2
- DOI:
- 10.4238/2013.October.7.2
Molecular characterization of the pseudorabies virus UL2 gene
Authors: M.S. Cai1,2, B.Y. Wang2, W. Cui1, Z.Y. Zhao1, J.H. Chen2, X.M. Wen1, Z. Li1 and M.L. Li1
A 948-bp sequence of the UL2 gene was amplified from the pseudorabies virus (PRV) Becker strain genome using polymerase chain reaction, and the gene identity was confirmed through further cloning and sequencing. Bioinformatic analysis indicated that the PRV UL2 gene encodes a putative polypeptide with 315-amino acid residues. Its encoding protein, designa.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.1
- DOI:
- 10.4238/2013.October.7.1
Expression analysis of Gli1 and Gli2 in different tissues and muscle-derived cells of Qinchuan cattle
Authors: Y. Liu, H. Wang, G. Cheng B. Jiang and L. Zan
The Hedgehog (Hh) signaling pathway regulates the differentiation of many kinds of cells and plays a critical role in many embryonic and postnatal developmental processes. Gli1 and Gli2 are two transcription factors of the Hh signaling pathway. In this study, we used quantitative real-time polymerase chain reaction to detect the relative expression of Gli.. Read More»
- Genet. Mol. Res. 13(4):