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Microbial Genetics   Research Article

Potassium contributes to zinc stress tolerance in peach (Prunus persica) seedlings by enhancing photosynthesis and the antioxidant defense system

Authors: Z.Z. Song, C.L. Duan, S.L. Guo, Y. Yang, Y.F. Feng, R.J. Ma and M.L. Yu

Zinc (Zn) is considered to be a major industrial pollutant because excessive amounts can impair plant growth. In this paper, we found that peach ‘Yoshihime’ seedlings are promising Zn tolerant plants. However, heavy Zn toxicity (2 mM) damaged plant performance by disrupting biochemical processes, including photosynthesis, proline production, a.. Read More»

Genet. Mol. Res. 14(3):
2015.July.27.22
DOI:
10.4238/2015.July.27.22
Medical Genetics   Research Article

Relationship between EPHX2 gene polymorphisms and essential hypertension in Uygur, Kazakh, and Han

Authors: X.L. Zhu, L. Wang, Z. Wang, S.Z. Chen, W.Q. Zhang and M.M. Ma

We investigated the association between rs751141 polymorphisms in the EPHX2 gene and essential hypertension in Uygur, Kazakh, and Han subjects in Xinjiang, China. A total of 302 essential hypertensive patients in Uygur, 267 in Kazakh, and 368 in Han, as well as 323 normotensive controls in Uygur, 284 in Kazakh, and 348 in Han were enrolled in this study. .. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.15.11
DOI:
http://dx.doi.org/10.4238/2015.April.15.11
Medical Genetics   Research Article

Transcriptome sequencing and analysis of the coconut leaf beetle, Brontispa longissima

Authors: W. Yan, L. Liu, C.X. Li, S.C. Huang, Z.L. Ma, W.Q. Qin and Z.Q. Peng

The coconut leaf beetle, Brontispa longissima, is a destructive pest of palm plants. Although its ecological and biological characteristics are well understood, its genetic information remains largely unknown. To advance our understanding of its molecular basis of biology and ecology, we sequenced and analyzed its whole transcriptome by using high-through.. Read More»

Genet. Mol. Res. 14(3):
2015.July.28.2
DOI:
10.4238/2015.July.28.2
Animal Genetics   Research Article

Relative quantification of beta-casein expression in primary goat mammary epithelial cell lines

Authors: J. Ogorevc and P. Dov?

Primary mammary epithelial cell cultures were established from mammary tissue of lactating and non-lactating goats to assess the expression of beta-casein (CSN2) in vitro. Primary cell cultures were established by enzymatic digestion of mammary tissue and characterized using antibodies against cytokeratin 14, cytokeratin 18, and vimentin. The established .. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.15.12
DOI:
http://dx.doi.org/10.4238/2015.April.15.12
Human Genetics   Research Article

Association between methionine synthase reductase A66G polymorphism and primary infertility in Chinese males

Authors: X.Y. Li, J.Z. Ye, X.P. Ding, X.H. Zhang, T.J. Ma, R. Zhong and H.Y. Ren

We examined the association between the methionine synthase reductase (MTRR A66G), methylenetetrahydrofolate reductase (MTHFR C677T and A1298C), and methionine synthase (MS A2756G) genotypes and non-obstructive male infertility in a Chinese population. This case-control study included 162 infertile Chinese patients with azoospermia (N = 100) or oligoasthe.. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.15.13
DOI:
http://dx.doi.org/10.4238/2015.April.15.13
Plant Genetics   Research Article

Production of transgenic kiwifruit plants harboring the SbtCry1Ac gene

Authors: H.Y. Zhang, H.M. Liu and X.Z. Liu

The kiwifruit (Actinidia chinensis Planch.) is an economically and nutritionally important fruit crop that has a remarkably high vitamin C content and is popular throughout the world. However, kiwifruit plants are vulnerable to attack from pests, and effective pest control is urgently required. Transgenic kiwifruit plants containing the synthetic chimeric.. Read More»

Genet. Mol. Res. 14(3):
2015.July.28.16
DOI:
10.4238/2015.July.28.16
Microbial Genetics   Research Article

Polymorphisms in the methylene tetrahydrofolate reductase and methionine synthase reductase genes and their correlation with unexplained recurrent spontaneous abortion susceptibility

Authors: L. Zhu

We aimed to explore the correlation between unexplained recurrent spontaneous abortion and polymorphisms in the methylene tetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) genes. A case control study was conducted in 118 patients with unexplained recurrent spontaneous abortion (abortion group) and 174 healthy women (control group.. Read More»

Genet. Mol. Res. 14(3):
2015.July.28.19
DOI:
10.4238/2015.July.28.19
Medical Genetics   Research Article

Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA

Authors: G.J. Chang, H.M. Seyfert and X.Z. Shen

In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing th.. Read More»

Genet. Mol. Res. 14(3):
2015.July.28.20
DOI:
10.4238/2015.July.28.20
Plant Genetics   Research Article

Fragmentation effects and genetic diversity of the key semidecidual forest species Metrodorea nigra in Southwestern Brazil

Authors: R.M. Moraes Filho, F. Bonif?¡cio-Anacleto and A.L. Alzate-Marin

Studies of genetic diversity in plant species present in the remaining fragments of the Atlantic Forest are very important for understanding their resilience to such a degraded ecosystem. We analyzed the genetic diversity of 3 populations of the high-density understory species Metrodorea nigra St. Hill. (Rutaceae) located in forest remnants in the region .. Read More»

Genet. Mol. Res. 14(2):
http://dx.doi.org/2015.April.15.15
DOI:
http://dx.doi.org/10.4238/2015.April.15.15
Animal Genetics   Research Article

Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers

Authors: Y. Zhao, Y. Li, Y. Liu and Y.F. Yang

Simple-sequence repeat (SSR) markers were employed to assess the genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China. Fourteen SSR primer pairs generated a total of 94 alleles (90 were polymorphic, accounting for 95.74%), with a mean of 6.71 alleles per locus. The number of alleles detected at each locus ranged from 2 at BPPCT .. Read More»

Genet. Mol. Res. 14(3):
2015.July.28.7
DOI:
10.4238/2015.July.28.7