Se
Sequence variants in the bovine PRDM16 gene associated with body weight in Chinese cattle breeds
Authors: J. Wang, C. Wang, R. Tian, Y.-Z. Huang, X.-S. Lai, X.-Y. Lan, J.-Q. Wang and H. Chen
As a zinc-finger protein, PR domain containing 16 (PRDM16) controls brown fat determination by stimulating brown fat cell production while suppressing the expression of genes for production of white fat cells; mutations in this domain are associated with myelodysplastic syndrome and leukemogenesis. In our study, polymorphisms .. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.22.5
- DOI:
- 10.4238/2012.March.22.5
Genetic variation in a wild population of the �sleep� passion fruit (Passiflora setacea) based on molecular markers
Authors: C.B.M. Cerqueira-Silva, E.S.L. Santos, L.D.H.C.S. Concei�§�£o, C.B. Cardoso-Silva, A.S. Pereira, A.C. Oliveira and R.X. Corr�ªa
Little is known about the molecular genetic diversity of most Passiflora species. We used RAPD markers to evaluate the genetic diversity of 24 genotypes of the ‘sleep’ passion fruit (Passiflora setacea). Twelve primers generated 95 markers, 88% of which were polymorphic. The genetic distance estimated by the comple.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.22.3
- DOI:
- 10.4238/2012.March.22.3
Application of vacuum-assisted closure in seawater-immersed wound treatment under different negative pressures
Authors: L. Cao, M.M. Peng, J.J. Sun, X.C. Yu and B. Shi
The therapeutic effect of vacuum-assisted closure (VAC) has been confirmed in many types of complex wounds, but there are few relevant reports regarding seawater-immersed wounds. The aim of this study was to determine the effect of VAC on seawater-immersed wound healing under different negative pressures and explore the optimal negative pressure value. Fo.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.8.12
- DOI:
- 10.4238/2015.June.8.12
Genetic polymorphisms in extracellular superoxide dismutase Leu53Leu, Arg213Gly, and Ala40Thr and susceptibility to type 2 diabetes mellitus
Authors: Y.M. Yang, X.R. Xie and A.L. Jin
The most common type of endocrine disease is type 2 diabetes mellitus (T2DM); genetic factors contribute to the development to T2DM. In this study, we investigated the role of the Leu53Leu, Arg213Gly, and Ala40Thr polymorphisms in extracellular superoxide dismutase (EC-SOD) gene in the development of T2DM in a Chinese populati.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048418
- DOI:
- 10.4238/gmr15048418
DNase concentration assay to obtain DNA-free RNA from sugarcane leaves
Authors: J.A. Santos, G.A. Luz, K.P. Oliveira, L.F. Oliveira, A.S. Andrade J�ºnior, S.E.S. Valente and P.S.C. Lima
The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (<1%) can produce a false positive amplification signal in quantitative real-time PCR. For RNA contaminated with .. Read More»
- Genet. Mol. Res. 15(4):
- gmr15049073
- DOI:
- 10.4238/gmr15049073
Molecular characterization and expression pattern of an �±-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii
Authors: Molecular characterization and expression pattern of an �±-amylase gene (HcAmy) from the freshwater pearl mussel, Hyriopsis cumingii
The freshwater pearl mussel Hyriopsis cumingii is of commercial importance because it produces the freshwater pearl; however, knowledge about the molecular characterization and regulation mechanisms of α-amylase remains unknown for this species. In this study, the full-length cDNA of the α-amylase gene (.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.10
- DOI:
- 10.4238/2014.August.28.10
Characterization of EST-derived and non-EST simple sequence repeats in an F1 hybrid population of Vitis vinifera L.
Authors: E. Kayesh, N. Bilkish, G.S. Liu, W. Chen, X.P. Leng and J.G. Fang
Among different classes of molecular markers, expressed sequence tags (ESTs) are a new resource for developing simple sequence repeat (SSR) functional markers for genotyping and genetic mapping in F1 hybrid populations of Vitis vinifera L. Recently, because of the availability of an enormous amount of data for ESTs in the publ.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.31.2
- DOI:
- 10.4238/2014.March.31.2
Genetic parameters and prediction of genotypic values for root quality traits in cassava using REML/BLUP
Authors: E.J. Oliveira, F.A. Santana, L.A. Oliveira and V.S. Santos
The aim of this study was to estimate the genetic parameters and predict the genotypic values of root quality traits in cassava (Manihot esculenta Crantz) using restricted maximum likelihood (REML) and best linear unbiased prediction (BLUP). A total of 471 cassava accessions were evaluated over two years of cultivatio.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.13
- DOI:
- 10.4238/2014.August.28.13
Zinc finger protein A20 overexpression inhibits monocyte homing and protects endothelial cells from injury induced by high glucose
Authors: C.L. Hou, W. Zhang, Y. Wei, J.H. Mi, L. Li, Z.H. Zhou, W. Zeng and D.J. Ying
Diabetes mellitus causes vascular lesions and may ultimately lead to atherosclerosis. One of the earliest steps in the development of atherosclerotic lesions is the adhesion of monocytes to endothelial cells of the vessel wall. It is currently unknown whether zinc finger protein A20 is able to protect endothelial cells from in.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1102
- DOI:
- 10.4238/vol10-2gmr1102
Genetic characterization of hatchery populations of Korean spotted sea bass (Lateolabrax maculatus) using multiplex polymerase chain reaction assays
Authors: H.S. An, H.Y. Kim, J.B. Kim, D.S. Chang, K.D. Park, J.W. Lee, J.I. Myeong and C.M. An
The spotted sea bass, Lateolabrax maculatus, is an important commercial and recreational fishery resource in Korea. Aquacultural production of this species has increased because of recent resource declines, growing consumption, and ongoing government-operated stock release programs. Therefore, the genetic characterization of h.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.14
- DOI:
- 10.4238/2014.August.28.14