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Microbial Genetics   Research Article

Evaluation of cellulolytic activity in insect digestive fluids

Authors: L.-J. Su, H.-F. Zhang, X.-M. Yin, M. Chen, F.-Q. Wang, H. Xie, G.-Z. Zhang and A.-D. Song

Efficient and low-cost cellulolytic enzymes are urgently needed to degrade recalcitrant plant biomass during the industrial production of lignocellulosic biofuels. Here, the cellulolytic activities in the gut fluids of 54 insect species that belong to 7 different taxonomic orders were determined using 2 different substrates, carboxymethyl cellulose (CMC) .. Read More»

Genet. Mol. Res. 12(3):
2013.January.4.11
DOI:
10.4238/2013.January.4.11
Bioinformatics   Research Article

Development and characterization of microsatellite loci in a threatened marine fish, Cheilinus undulatus (humphead wrasse)

Authors: J. Hu, X.P. Zhu, J. Luo, S.W. Yin, Y.H. Peng, Y.L. Hu and F. Zhu

Cheilinus undulatus (humphead wrasse) is a marine fish distributed widely throughout the tropical Indo-Pacific. It has been listed as vulnerable in the IUCN Red Data Book and in CITES Appendix II four times. Fifteen microsatellite loci were isolated and characterized for this species. The number of alleles ranged from 3 to 15 per locus, and the observed a.. Read More»

Genet. Mol. Res. 12(3):
2013.July.30.2
DOI:
10.4238/2013.July.30.2
Plant Genetics   Research Article

Analysis of the relationship between ribosomal DNA ITS sequences and active components in Rhodiola plants

Authors: D.J. Zhang, W.T. Yuan, M.T. Li and Y.H. Zhang

Rhodiola plants are a valuable resource in traditional Chinese medicine. The objective of this study was to evaluate the correlation between ribosomal DNA internal transcribed spacer (ITS) sequences and the three active components in Rhodiola plants. For this, we determined ITS sequence polymorphisms and the concentrations of .. Read More»

Genet. Mol. Res. 15(4):
gmr15049337
DOI:
10.4238/gmr15049337
Human Genetics   Research Article

Cytogenetic analysis of 4216 patients referred for suspected chromosomal abnormalities in Southeast Turkey

Authors: M. Balkan, H. Akbas, H. Isi, D. Oral, A. Turky�±lmaz, S. Kalkanli, S. Simsek, M. Fidanboy, M.N. Alp, A. Gedik and T. Budak

We reviewed cytogenetic studies performed on 4216 patients who were referred to the Cytogenetics Unit at Dicle University Hospital, Diyarbair, Southeast Turkey, between 2000 and 2009. The cases were grouped according to the reason of referral for cytogenetic analysis. The frequencies of the different types of numerical and str.. Read More»

Genet. Mol. Res. 9(2):
vol9-2gmr827
DOI:
10.4238/vol9-2gmr827
Plant Genetics   Research Article

Transcriptome analysis of peach (Prunus persica L. Batsch) during the late stage of fruit ripening

Authors: H.F. Pan, Y. Sheng, Z.H. Gao, H.L. Chen, Y.J. Qi, X.K. Yi, G.H. Qin and J.Y. Zhang

Fruit ripening is a complex developmental process, the details of which remain largely unknown in fleshy fruits. In this paper, the fruit flesh of two peach varieties, “Zhongyou9” (a nectarine; Prunus persica L. Batsch) and its mutant “Hongyu”, was analyzed by RNA-seq technology during two stages of rip.. Read More»

Genet. Mol. Res. 15(4):
gmr15049335
DOI:
10.4238/gmr15049335
Microbial Genetics   Research Article

Optimization of virus-induced gene silencing in pepper (Capsicum annuum L.)

Authors: J.-E. Wang, D.-W. Li, Z.-H. Gong and Y.-L. Zhang

Virus-induced gene silencing is currently a powerful tool for the study of gene function in plants. Here, we optimized the protocol for virus-induced gene silencing, and investigated factors that affect the efficiency of tobacco rattle virus-induced gene silencing in pepper plants. Consequently, an optimal protocol was obtained by the syringe-infiltration.. Read More»

Genet. Mol. Res. 12(3):
2013.July.24.4
DOI:
10.4238/2013.July.24.4
Plant Genetics   Research Article

Understanding bamboo flowering based on large-scale analysis of expressed sequence tags

Authors: X.C. Lin, T.Y. Chow, H.H. Chen, C.C. Liu, S.J. Chou, B.L. Huang, C.I. Kuo, C.K. Wen, L.C. Huang and W. Fang

Unlike other plants, bamboo (Bambusoideae) flowering is an elusive physiological phenomena, because it is unpredictable, long-periodic, gregarious, and uncontrollable; also, bamboo plants usually die after flowering. The flowering mechanism in Arabidopsis thaliana, a eudicot model species, is well established, but it remains u.. Read More»

Genet. Mol. Res. 9(2):
vol9-2gmr804
DOI:
10.4238/vol9-2gmr804
Animal Genetics   Research Article

Heat and chemical stress modulate the expression of the �±-RYR gene in broiler chickens

Authors: I.L. Ziober, F.G. Pai�£o, D.F. Marchi, L.L. Coutinho, E. Binneck, A.L. Nepomuceno and M. Shimokomaki

The biological cause of Pork Stress syndrome, which leads to PSE (pale, soft, exudative) meat, is excessive release of Ca2+ ions, which is promoted by a genetic mutation in the ryanodine receptors (RyR) located in the sarcoplasmic reticulum of the skeletal muscle cells. We examined the relationship between the formation of PSE.. Read More»

Genet. Mol. Res. 9(2):
vol9-2gmr830
DOI:
10.4238/vol9-2gmr830
Animal Genetics   Research Article

mRNA abundance and expression of SLC27A, ACC, SCD, FADS, LPIN, INSIG, and PPARGC1 gene isoforms in mouse mammary glands during the lactation cycle

Authors: L.Q. Han, H.J. Li, Y.Y. Wang, H.S. Zhu, L.F. Wang, Y.J. Guo, W.F. Lu, Y.L. Wang and G.Y. Yang

The functions of distinct isoforms of solute carrier family 27 transporters (SLC27A1-6), acetyl-CoA carboxylase (ACACA, ACACB), stearoyl-CoA desaturase (SCD1-4), fatty acid desaturase (FADS1-3), LPIN (LPIN1-3), insulin-induced gene (INSIG1, 2), and peroxisome proliferator-activated receptor gamma coactivator1 (PPARGC1A, B) wer.. Read More»

Genet. Mol. Res. 9(2):
vol9-2gmr814
DOI:
10.4238/vol9-2gmr814
Cytogenomics   Research Article

Association of novel single nucleotide polymorphisms of the CXCR1 gene with the milk performance traits of Chinese native cattle

Authors: L. Zhou, H.M. Wang, Z.H. Ju, Y. Zhang, J.M. Huang, C. Qi, M.H. Hou, L.G. An, J.F. Zhong and C.F. Wang

Mastitis is an economically devastating disease affecting the dairy industry. Dairy cows with mastitis give reduced milk yield and produce milk that is unfit for consumption. The chemokine receptor CXCR1 is an excellent prospective genetic marker for mastitis resistance in cattle because it regulates neutrophil migration, killing, and survival during infe.. Read More»

Genet. Mol. Res. 12(3):
2013.July.30.10
DOI:
10.4238/2013.July.30.10