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Identification and characterization of conserved microRNAs and their target genes in wheat (Triticum aestivum)
Authors: Z.J. Yin and F.F. Shen
MicroRNAs (miRNAs) are non-coding small RNAs that regulate gene expression by translational repression or transcript degradation. A large number of miRNAs have been identified from model plant species; however, the character of conserved miRNAs is poorly understood. We studied 42 miRNA families that are conserved within the pl.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr805
- DOI:
- 10.4238/vol9-2gmr805
Variation of the OsGI intron and its phenotypic associations in Oryza rufipogon Griff. and Oryza sativa L.
Authors: Y. Dong, Z. Chen, X. Pei, F. Wang, Q. Yuan, H. Wu, S. Jia and Y. Peng
We analyzed intron 9 of the OsGI gene in Oryza rufipogon and Oryza sativa in order to investigate evolutionary relationships in rice and the relationship between intron variation and phenotype. OsGI-9 was cloned in 38 O. rufipogon populations and in 139 O. sativa cultivars and the phylogeny was reconstructed. Seed cold tolerance and dormancy were quantifi.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.4
- DOI:
- 10.4238/2013.July.30.4
Survey of simple sequence repeats in woodland strawberry (Fragaria vesca)
Authors: L. Guan, J.F. Huang, G.Q. Feng, X.W. Wang, Y. Wang, B.Y. Chen and Y.S. Qiao
The use of simple sequence repeats (SSRs), or microsatellites, as genetic markers has become popular due to their abundance and variation in length among individuals. In this study, we investigated linkage groups (LGs) in the woodland strawberry (Fragaria vesca) and demonstrated variation in the abundances, densities, and relative densities of mononucleot.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.3
- DOI:
- 10.4238/2013.July.30.3
Significance of linkage disequilibrium heterogeneous patterns in the 21q22.3 region for mapping 21 trisomy individualsv
Authors: Y. Valle, J.R. Padilla-GutiÃ?©rrez, A. Quintero-Ramos, I.J. GarcÃ?Âa-GonzÃ?¡lez and F. Rivas
Recombination patterns can be indirectly inferred by means of linkage disequilibrium (LD) estimates, since LD is negatively correlated with genetic distance. However, LD does not necessarily have absolute correspondence with genetic distance. We estimated LD at 5 loci located in the 21q22.3 region. These STRs (D21S1440, D21S168, D21S1260, D21S1446, and D2.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.8.2
- DOI:
- 10.4238/2013.August.8.2
Novel tetranucleotide microsatellite markers for Chinese beard eel (Cirrhimuraena chinensis Kaup)
Authors: H. Li, T.M. Ge, S.X. Ding and H.J. Wang
In this study, we characterized 12 novel polymorphic microsatellite loci for Chinese beard eel (Cirrhimuraena chinensis Kaup) from a tetranucleotide microsatellite-enriched library. Loci screened on a sample of 37 individuals from Xiamen wild stocks revealed 8 to 24 alleles per locus, with a mean of 13.83 over all loci. Observed and expected heterozygosit.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.2.3
- DOI:
- 10.4238/2013.August.2.3
Gene knockout by inducing P-element transposition in Drosophila
Authors: H.L. Ou
The P-element has been successfully used in germline transformation to create transgenic flies and as an insertion mutagenesis agent to disrupt genes. Moreover, P-element can also be used to knockout genes that are near the insertion sites by inducing its imprecise transposition. In this article, P-element insertion lines were crossed with transposase exp.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.20
- DOI:
- 10.4238/2013.January.4.20
Single-nucleotide polymorphism of the pri-miR-34b/c gene is not associated with susceptibility to congenital heart disease in the Han Chinese population
Authors: Y.-M. Liu, Y. Wang, W. Peng, Z. Wu, X.-H. Wang, M.-L. Wang, W. Wang, J. Sun, Z.-D. Zhang and X.-M. Mo
Recent evidence has shown that the microRNA polymorphism may play an important role in the susceptibility to congenital heart disease (CHD). A potentially functional SNP rs4938723 (T>C) in the promoter region of pri-miR-34b/c might affect transcription factor GATA binding and therefore pri-miR-34b/c expression. We genotyped the pri-miR-34b/c polymorphi.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.9
- DOI:
- 10.4238/2013.August.12.9
Protein synthesis and degradation gene SNPs related to feed intake, feed efficiency, growth, and ultrasound carcass traits in Nellore cattle
Authors: R.C. Gomes, S.L. Silva, M.E. Carvalho, F.M. Rezende, L.F.B. Pinto, M.H.A. Santana, T.R. Stella, F.V. Meirelles, P. Rossi J�ºnior, P.R. Leme and J.B.S. Ferraz
We looked for possible associations of SNPs in genes related to protein turnover, with growth, feed efficiency and carcass traits in feedlot Nellore cattle. Purebred Nellore bulls and steers (N = 290; 378 ± 42 kg body weight, 23 months ± 42 days old) were evaluated for daily feed intake, body weight gain (BWG), gross feed efficiency, feed co.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.8
- DOI:
- 10.4238/2013.August.12.8
Assessing the genetic relationships of Curcuma alismatifolia varieties using simple sequence repeat markers
Authors: S. Taheri, T.L. Abdullah, N.A.P. Abdullah, Z. Ahmad, E. Karimi and M.R. Shabanimofrad
The genus Curcuma is a member of the ginger family (Zingiberaceae) that has recently become popular for use as flowering pot plants, both indoors and as patio and landscape plants. We used PCR-based molecular markers (SSRs) to elucidate genetic variation and relationships between five varieties of Curcuma (.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.5.12
- DOI:
- 10.4238/2014.September.5.12
Identification of apple cultivars on the basis of simple sequence repeat markers
Authors: G.S. Liu, Y.G. Zhang, R. Tao, J.G. Fang and H.Y. Dai
DNA markers are useful tools that play an important role in plant cultivar identification. They are usually based on polymerase chain reaction (PCR) and include simple sequence repeats (SSRs), inter-simple sequence repeats, and random amplified polymorphic DNA. However, DNA markers were not used effectively in the complete ide.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.3
- DOI:
- 10.4238/2014.September.12.3