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Cytogenomics   Research Article

Chromosomal characteristics of a Brazilian whip spider (Amblypygi) and evolutionary relationships with other arachnid orders

Authors: E. Paula-Neto, D. Araujo, L.S. Carvalho, D.M. Cella and M.C. Schneider

We analyzed mitotic and meiotic cells of a Brazilian amblypygid, Heterophrynus longicornis, using conventional and molecular cytogenetic techniques (Giemsa staining, C-banding, Ag-NOR, and FISH with rDNA probe). This is the first study that focuses solely on amblypygid chromosomes; it was undertaken to add data on cytogenetic knowledge of this group and c.. Read More»

Genet. Mol. Res. 12(3):
2013.September.19.3
DOI:
10.4238/2013.September.19.3
Cytogenomics   Research Article

Potential role of Atp5g3 in epigenetic regulation of alcohol preference or obesity from a mouse genomic perspective

Authors: Y. Huang, L. Wang, B. Bennett, R.W. Williams, Y.J. Wang, W.K. Gu and Y. Jiao

The mitochondrial ATP synthase, subunit c, isoform 3 gene (Atp5g3) encodes subunit 9, the subunit of the multisubunit enzyme that catalyzes ATP synthesis during oxidative phosphorylation in mitochondria. According to the Ensembl database, Atp5g3 in mice is located on chromosome 2 between 73746504 and 73749383 bp, within the genomic regions of two sets of .. Read More»

Genet. Mol. Res. 12(3):
2013.September.18.1
DOI:
10.4238/2013.September.18.1
Cytogenomics   Research Article

Breeding of transgenic cattle for human coagulation factor IX by a combination of lentiviral system and cloning

Authors: P.S. Monzani, J.R. Sangalli, T.H.C. De Bem, F.F. Bressan, P. Fantinato-Neto, J.R.V. Pimentel, E.H. Birgel-Junior, A.M. Fontes, D.T. Covas and F.V. Meirelles

Recombinant coagulation factor IX must be produced in mammalian cells because FIX synthesis involves translational modifications. Human cell culture-based expression of human coagulation factor IX (hFIX) is expensive, and large-scale production capacity is limited. Transgenic animals may greatly increase the yield of therapeutic proteins and reduce costs... Read More»

Genet. Mol. Res. 12(3):
2013.February.28.25
DOI:
10.4238/2013.February.28.25
Bioinformatics   Research Article

Development of polymorphic microsatellite markers for Dioscorea zingiberensis and cross-amplification in other Dioscorea species

Authors: Q.-Q. Yan, X.-Q. Sun, J.-L. Guo, Y.-Y. Hang and M.-M. Li

Dioscorea zingiberensis C.H. Wright (Dioscoreaceae) is an endemic species in central and southwestern China. In order to study the genetic diversity and population structure of this species, 19 novel polymorphic microsatellite loci were developed using a dual-suppression PCR technique. The number of alleles per locus ranged from 3 to 21, with an average o.. Read More»

Genet. Mol. Res. 12(3):
2013.September.19.10
DOI:
10.4238/2013.September.19.10
Human Genetics   Research Article

Genetic structure of Aedes aegypti populations determined using pairwise comparisons

Authors: T. de F. Patarro, M.M. Guirado, L.M. Ravazzi and H.E.M. de C. Bicudo

The biological characteristics of Aedes aegypti (Diptera, Culicidae), which is a vector of dengue and yellow fever, make this organism a good model for studying population structure and the events that may influence it under the effect of human activity. We assessed the genetic variability of five A. aegypti populations using RAPD-PCR technique and six pr.. Read More»

Genet. Mol. Res. 12(3):
2013.September.19.9
DOI:
10.4238/2013.September.19.9
Human Genetics   Research Article

Microsatellite markers reveal genetic divergence among wild and cultured populations of Chinese sucker Myxocyprinus asiaticus

Authors: W.W. Cheng, D.Q. Wang, C.Y. Wang, H. Du and Q.W. Wei

Studies of genetic diversity and genetic population structure are critical for the conservation and management of endangered species. The Chinese sucker Myxocyprinus asiaticus is a vulnerable monotypic species in China, which is at a risk of decline owing to fluctuations in effective population size and other demographic and e.. Read More»

Genet. Mol. Res. 15(2):
gmr.15027581
DOI:
10.4238/gmr.15027581
Medical Genetics   Research Article

Xuemaitong granules attenuate carotid atherosclerosis by decreasing the expression of CD14+CD16+ monocytes, IL-6, TNF-�±, and hsCRP

Authors: Q. Zhang, G. Qian and Z. Ding

Carotid atherosclerosis (CAS) has been extensively studied because its position can be easily observed. Our objective was to investigate the effects of Xuemaitong granules on the generation and activation of CD14+CD16+ monocytes on the inflammatory reaction in CAS patients. In this study, 22 male apolipoprotein E (apoE)-defici.. Read More»

Genet. Mol. Res. 13(3):
2014.September.12.19
DOI:
10.4238/2014.September.12.19
Microbial Genetics   Research Article

Composition and structure of microbial communities associated with different domestic sewage outfalls

Authors: Z.H. Wang, J.Q. Yang, D.J. Zhang, J. Zhou, C.D. Zhang, X.R. Su and T.W. Li

The diversity of microbiota in waste waters has not been thoroughly examined, despite the potential impact of microbes on effluent quality. Wastewater microbial communities harbor pathogenic bacteria, viruses, and parasites. To study microbial communities in domestic sewage outfalls, 454 pyrosequencing technology was used to i.. Read More»

Genet. Mol. Res. 13(3):
2014.September.12.21
DOI:
10.4238/2014.September.12.21
Plant Genetics   Research Article

Genetic diversity of Broussonetia papyrifera populations in southwest China

Authors: S.X. Liao, Z.H. Deng, K. Cui, Y.Z. Cui and C.H. Zhang

Broussonetia papyrifera is an important native tree species with high economic value in southwest China. Its resources are drastically reduced because of over-harvesting and habitat fragmentation. In this study, 17 natural populations of B. papyrifera were analyzed using inter-simple sequence repeat (ISSR) ma.. Read More»

Genet. Mol. Res. 13(3):
2014.September.12.22
DOI:
10.4238/2014.September.12.22
Animal Genetics   Short communication

Diversity of TNF-�± region in Chinese domestic goats

Authors: G.X. E,R.S. Na,Y.J. Zhao,Y.H. Ma and Y.F. Huang

Tumor necrosis factor-a is a cytokine with a wide range of effects on both lymphoid and non-lymphoid cells. In this study, we identified polymorphisms in major histocompatibility complex class III gene in the 4th exon and the 3' untranslated region of tumor necrosis factor-a to evaluate the immunogenetic diversity of Chinese south indigenous goat. Three s.. Read More»

Genet. Mol. Res. 14(2):
2015.April.17.9
DOI:
10.4238/2015.April.17.9