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A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression
Authors: H. Li, Q. Jiang, Z.H. Ju, J.M. Huang, X.G. Wang, C.H. Yang, Y. Sun, G.W. Yang, J.F. Zhong and C.F. Wang
The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is a newly identified protein that is specifically expressed in testis tissue and participates in spermatogenesis. In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain react.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027723
- DOI:
- 10.4238/gmr.15027723
Development of novel SCAR markers for genetic characterization of Lonicera japonica from high GC-RAMP-PCR and DNA cloning
Authors: J.L. Cheng, J. Li, Y.M. Qiu, C.L. Wei, L.Q. Yang and J.J. Fu
Sequence-characterized amplified region (SCAR) markers were further developed from high-GC primer RAMP-PCR-amplified fragments from Lonicera japonica DNA by molecular cloning. The four DNA fragments from three high-GC primers (FY-27, FY-28, and FY-29) were successfully cloned into a pGM-T vector. The positive clones were seque.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027737
- DOI:
- 10.4238/gmr.15027737
Therapeutic influence of intraperitoneal injection of Wharton�s jelly-derived mesenchymal stem cells on oviduct function and fertility in rats with acute and chronic salpingitis
Authors: H.J. Luo,X.M. Xiao,J. Zhou and W. Wei
To study the effect of Wharton’s jelly-derived mesenchymal stem cells (WJMSCs) on the rat salpingitis model, 50 female Wistar rats were randomly divided into one control and five model groups. Mixed bacteria were injected into the oviducts of model groups. The treated acute and chronic groups received intraperitoneal injections of WJMSCs (1 x 106) o.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.17.10
- DOI:
- http://dx.doi.org/10.4238/2015.April.17.10
Dynamic changes of virus load in supernatant of primary CEK cell culture infected with different generations of avian infectious bronchitis virus strains Sczy3 as revealed by real-time reverse transcription-polymerase chain reaction
Authors: Z.J. Wei, F.Y. Wang, M.P. Guo, Z.Z. Duan, N.L. Zou, P. Liu, Q.G. Yan, X.T. Wen, S.J. Cao and Y. Huang
Infectious bronchitis virus (IBV) can multiply effectively in chick embryo kidney (CEK) cells after adapting to the chick embryo. To investigate the dynamic changes in IBV load in the supernatant of primary CEK cells, we developed an SYBR Green I-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the IBV-Sczy3 strain. The .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.9
- DOI:
- 10.4238/2015.June.11.9
Whole-exome analysis of a child with polycystic kidney disease and ventriculomegaly
Authors: M.M. Nabhan,H. Abdelaziz,Y. Xu,R. El Sayed,M. Santibanez-Koref,N.A. Soliman and J.A. Sayer
Autosomal recessive polycystic kidney disease (ARPKD) is an inherited ciliopathy leading to progressive kidney and liver disease. Biallelic mutations in the PKHD1 gene underlie this condition. We describe a child with bilaterally enlarged cystic kidneys, portal hypertension, and cerebral ventriculomegaly. Molecular genetic investigations using whole-exome.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.17.11
- DOI:
- http://dx.doi.org/10.4238/2015.April.17.11
Positive association between PPARD rs2016520 polymorphism and coronary heart disease in a Han Chinese population
Authors: H.D. Ye, Y.R. Li, Q.X. Hong, A.N. Zhou, Q.L. Zhao, L.M. Xu, M.Q. Xu, X.T. Xu, L.L. Tang, D.J. Dai, D.J. Jiang, Y. Huang,D.W. Wang and S.W. Duan
PPARD encodes peroxisome proliferator-activated receptor delta, which has been shown to play an important role in controlling lipid metabolism and atherosclerosis. In this case-control study, we explored the relationship between PPARD rs2016520 polymorphism and coronary heart disease (CHD) in a Han Chinese population. A total of 657 CHD cases and 640 cont.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.10
- DOI:
- 10.4238/2015.June.11.10
Activity of matrix metalloproteinases 2 and 9 in cultured rabbit corneal epithelium cells stimulated by tumor necrosis factor alpha
Authors: Z.-Q. Wu, Z.-L. Zhang, S.-W. Nie, J. Yuan and Y.-N. Yang
We studied the activity of matrix metalloproteinases (MMP) 2 and 9 generated by cultured rabbit corneal epithelium cells that had been stimulated with tumor necrosis factor alpha (TNF-a), to investigate the possible regulative mechanisms of MMP-2/9 and their potential effect on corneal inflammatory diseases. The rabbit corneal epithelium cells were cultur.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.11
- DOI:
- 10.4238/2015.June.11.11
Cloning and analysis of the soybean MEKK gene
Authors: A.-H. Sha,H.-P. Ba,Z.-H. Shan,H.-F. Chen,S.-L. Chen,D.-Z. Qiu,X.-A. Zhou and Y.-H. Chen
In this paper, homologous cloning methods were used to clone the soybean GmMEKK gene, which possesses a high degree of similarity to Arabidopsis thaliana AtMEKK1. AtMEKK1 is formed by 595 amino acids, and its secondary structure is formed by 38 irregular curls, 24 α helix, 14 β, with S-TKc domain, transmembrane domain and does not have membrane.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.17.12
- DOI:
- http://dx.doi.org/10.4238/2015.April.17.12
A simplified genomic DNA extraction protocol for pre-germination genotyping in rice
Authors: Y.B. Duan, F.L. Zhao, H.D. Chen, H. Li, D.H. Ni, P.C. Wei, W. Sheng, J.T. Teng, A.M. Zhang and J.P. Xue
Genotyping is a critical step for molecular markerassisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, whi.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.12
- DOI:
- 10.4238/2015.June.11.12
Effect of Ginkgo biloba extract on apoptosis of brain tissues in rats with acute cerebral infarction and related gene expression
Authors: C. Wu, X. Zhao, X. Zhang, S. Liu, H. Zhao and Y. Chen
We investigated the effect of Ginkgo biloba extract on apoptosis of brain tissues in rats with acute cerebral infarction and apoptosis-related gene expression. Rat models of acute cerebral infarction were constructed using the suture method, and randomly divided into the control group, model, and treatment groups. In the treatment group, 4 mg/kg G. biloba.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.14
- DOI:
- 10.4238/2015.June.11.14