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Sex identification based on AMEL gene PCR amplification from blue sheep (Pseudois nayaur) fecal DNA samples
Authors: X. Liu, Y.Y. Yang, X.M. Wang, Z.S. Liu, Z.H. Wang and Y.Z. Ding
The use of noninvasive genetic sampling to identify the sex of wild animals is an extremely valuable and important tool in molecular ecology and wildlife conservation. Sex determination using the amelogenin gene has been conducted in many species because only a single pair of primers is required to amplify both X- and Y-linked alleles. However, this metho.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.13
- DOI:
- 10.4238/2015.August.7.13
Use of microsatellite markers to assign goats to their breeds
Authors: R.S. Aljumaah, M.M. Alobre and R.M. Al-Atiyat
We investigated the potential of 17 microsatellite markers for assigning Saudi goat individuals to their breeds. Three local breeds, Bishi, Jabali, and Tohami were genotyped using these markers, and Somali goats were used as a reference breed. The majority of alleles were shared between the breeds, except for some that were specific to each breed. The Gar.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.16
- DOI:
- 10.4238/2015.August.7.16
Complete mitochondrial genome of the Chinese Hwamei Garrulax canorus (Aves: Passeriformes): the first representative of the Leiothrichidae family with a duplicated control region
Authors: D.S. Chen, C.J. Qian, Q.Q. Ren, P. Wang, J. Yuan, L. Jiang, D. Bi, Q. Zhang, Y. Wang and X.Z. Kan
The Chinese Hwamei Garrulax canorus, a member of the family Leiothrichidae, is commonly found in central and southern China, northern Indochina, and on Hainan Island. In this study, we sequenced the complete mitochondrial genome of G. canorus. The circular mitochondrial genome is 17,785 bp in length and includes 13 protein-coding genes, 22 transfer RNA (t.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.5
- DOI:
- 10.4238/2015.August.7.5
Molecular characterization of a Han Chinese family with essential hypertension
Authors: J.F. Zhu, X. Zhang and L. Ling
Mutations in the mitochondrial genome have been found to be associated with essential hypertension. Here, we report the clinical and molecular characterization of a three-generation Han Chinese family with maternally inherited hypertension. Most strikingly, this pedigree exhibited a high penetrance of hypertension. Sequence an.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028084
- DOI:
- 10.4238/gmr.15028084
Adaptability of soybean cultivars in different crop years
Authors: I.O. Soares, P.M. Rezende, A.T. Bruzi, E.V. Zambiazzi, A.M. Zuffo, K.B. Silva and R. Gwinner
Soybean is one of the main sources of foreign exchange credits for Brazil in the agricultural sector. There is increasing interest in growing this leguminous crop, especially in the southern region of Minas Gerais, due to its importance as an alternative for crop rotation with maize. In this respect, the study of the adaptability of new cultivars to the r.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.8
- DOI:
- 10.4238/2015.August.7.8
Association between a microRNA-214 binding site polymorphism in the methylenetetrahydrofolate reductase gene and esophageal squamous cell carcinoma
Authors: G.R. Shen, W.Z. Li, Y.C. Liu, X.P. Li and H.Y. Yuan
MicroRNAs (miRNAs) are key regulators of gene expression and play an important role in the development and progression of various diseases including esophageal squamous cell carcinoma (ESCC). In this study, we determined whether a polymorphism at the miR-214 binding site in the 3'-untranslated region (3'-UTR) of the methylenet.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028158
- DOI:
- 10.4238/gmr.15028158
Characteristic element of matrix attachment region mediates vector attachment and enhances nerve growth factor expression in Chinese hamster ovary cells
Authors: X.Y. Wang, J.H. Zhang, Q.L. Sun, Z.Y. Yao, B.G. Deng, W.Y. Guo,L. Wang, W.H. Dong, F. Wang, C.P. Zhao and T.Y. Wang
Preliminary studies have suggested that a characteristic element of the matrix attachment region (MAR) in human interferon-β mediates the adhesion of vectors to Chinese hamster ovary (CHO) cells. In this study, we investigated if vector adhesion increased nerve growth factor (NGF) expression in CHO cells. The MAR characteristic element sequence of hu.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.29
- DOI:
- 10.4238/2015.August.7.29
QTL mapping of forage yield and forage yield component traits in Sorghum bicolor x S. sudanense
Authors: Y.L. Liu,L.H. Wang,J.Q. Li,Q.W. Zhan,Q. Zhang,J.F. Li and F.F. Fan
The sorghum-sudangrass hybrid (Sorghum bicolor x S. sudanense) is an important forage crop. However, little is known about the genetic mechanisms related to forage yield and the 4 forage yield component traits in this forage crop. In this study, a linkage map was constructed with 124 assigned SSR markers using an F2 mapping population derived from the cro.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.22.14
- DOI:
- http://dx.doi.org/10.4238/2015.April.22.14
Diadenylate cyclase evaluation of ssDacA (SSU98_1483) in Streptococcus suis serotype 2
Authors: B. Du and J.H. Sun
Cyclic diadenosine monophosphate is a recently identified signaling molecule. It has been shown to play important roles in bacterial pathogenesis. SSU98_1483 (ssDacA), which is an ortholog of Listeria monocytogenes DacA, is a putative diadenylate cyclase in Streptococcus suis serotype 2. In this study, we determined the enzymatic activity of ssDacA in vit.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.34
- DOI:
- 10.4238/2015.June.18.34
Protective effects of morphine preconditioning in delayed phase on myocardial ischemia-reperfusion injury in rabbits
Authors: X.-H. Lu, K. Ran, Y.-Y. Xiao, D.-L. Yang, Y.-T. Chang, K.-M. Duan and Y.-W. Ou
The aim of this study was to investigate the protective mechanisms of delayed-phase morphine preconditioning on myocardial ischemia-reperfusion injury. Thirty healthy male New Zealand white rabbits were randomly divided into three groups: a sham operation group (C), ischemia-reperfusion group (I/R), and delayed-phase morphine preconditioning group (M) (N .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.3
- DOI:
- 10.4238/2015.August.7.3