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ATP-dependent chromatin remodeling and histone acetyltransferases in 5-FU cytotoxicity in Saccharomyces cerevisiae
Authors: R. Matuo, F.G. Sousa, D. Bonatto, A.A. Mielniczki-Pereira, J. Saffi, D.G. Soares, A.E. Escargueil, A.K. Larsen and J.A.P. Henriques
Chromatin is thought to modulate access of repair proteins to DNA lesions, and may be altered by chromatin remodelers to facilitate repair. We investigated the participation of chromatin remodelers and DNA repair in 5-fluorouracil (5-FU) cytotoxicity in Saccharomyces cerevisiae. 5-FU is an antineoplastic drug commonly used in clinical settings. Among the .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.26.6
- DOI:
- 10.4238/2013.April.26.6
Detection and characterization of carbendazim resistance in Sclerotinia sclerotiorum isolates from oilseed rape in Anhui Province of China
Authors: D. Xu, Y. Pan, H. Zhang, X. Li, Y. Dai, S. Cao and Z. Gao
Stem rot caused by Sclerotinia sclerotiorum is a devastating disease of oilseed rape (Brassica napus) in Anhui Province of China. The fungicide carbendazim (methyl benzimidazole-2-yl carbamate; MBC) has been used to control this fungal disease since the 1980s. In the present study, 74 isolates of S. sclerotiorum from 13 regions of Anhui were collected, an.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.11.10
- DOI:
- 10.4238/2015.December.11.10
Result and pedigree analysis of spontaneously abortion villus chromosome detecting by FISH
Authors: N. An, L.-L. Li, X.-Y. Zhang, W.-T. Sun, M.-H. Liu and R.-Z. Liu
The aim of this study was to evaluate the relationship between fetal karyotype and parental chromosomal abnormalities, and to provide a basis for clinical diagnosis and therapy in Northeast China. A total of 144 spontaneously aborted fetuses were analyzed by FISH to test for chromosome number and to recall couples for peripheral blood karyotype analysis. .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.11.13
- DOI:
- 10.4238/2015.December.11.13
Genes associated with disc degeneration identified using microarray gene expression profiling and bioinformatics analysis
Authors: Y. Chen, K. Chen, M. Li, C. Li, H. Ma, Y.S. Bai, X.D. Zhu and Q. Fu
Disc degeneration is strongly associated with back or neck pain, sciatica, and disc herniation or prolapse. It places an enormous economic burden on society and can greatly affect quality of life. Alternative treatment approaches, such as genetic therapies, are urgently needed to slow or reverse the disc degeneration process. We downloaded gene expression.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.26.5
- DOI:
- 10.4238/2013.April.26.5
Serum cytokine modulation after Staphylococcus hyicus infection in BALB/c mice
Authors: Y. Li, L. Wang, L.Y. Zhang, Y.L. Liu, Z.Y. Jiang1, R.J. Cai and C.X. Song
Staphylococcal infection is a severe bacterial infection. Finding satisfactory predictable biological markers is essential for the treatment of this condition. In this study, we applied a 32-marker sandwich ELISA-based antibody array to evaluate cytokine changes in Staphylococcus hyicus-inoculated BALB/c mice at 24 and 48 h post infection. Among the cytok.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.11.16
- DOI:
- 10.4238/2015.December.11.16
Evaluation of the stability of reference genes in bone mesenchymal stem cells from patients with avascular necrosis of the femoral head
Authors: X.N. Wang, Q.W. Yang, Z.W. Du, T. Yu, Y.G. Qin, Y. Song, M. Xu and J.C. Wang
This study aimed to evaluate 12 genes (18S, GAPDH, B2M, ACTB, ALAS1, GUSB, HPRT1, PBGD, PPIA, PUM1, RPL29, and TBP) for their reliability and stability as reference sequences for real-time quantitative PCR (RT-qPCR) in bone marrow-derived mesenchymal stem cells (BMSCs) isolated from patients with avascular necrosis of the femo.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027926
- DOI:
- 10.4238/gmr.15027926
Peroxidase is involved in Pepper yellow mosaic virus resistance in Capsicum baccatum var. pendulum
Authors: L.S.A. Gon�§alves, R. Rodrigues, M.S.S. Diz, R.R. Robaina, A.T. do Amaral J�ºnior, A.O. Carvalho and V.M. Gomes
Pathogenesis-related proteins (PRs) are among the defense mechanisms of plants that work as an important barrier to the development of pathogens. These proteins are classified into 17 families according to their amino acid sequences, serology, and/or biological or enzyme activity. The present study aimed to identify PRs associated with the pathosystem of .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.26.3
- DOI:
- 10.4238/2013.April.26.3
Blockage of cisplatin-induced autophagy sensitizes cervical cancer cells to cisplatin
Authors: W.M. Lin and Z.G. Li
Development of chemoresistance is a major obstacle that leads to the recurrence and progression of cervical cancer (CC). Autophagy, meaning, “eating of self”, has shown paradoxical functions in tumors. In this study, we first investigated the process of autophagy induction by cisplatin in CC cells. Next, we investigated the role of autophagy i.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.18
- DOI:
- 10.4238/2015.December.14.18
Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG
Authors: J. Ma, M.G. Wang, A.H. Mao, J.Y. Zeng, Y.Q. Liu, X.Q. Wang, J. Ma, Y.J. Tian, N. Ma, N. Yang, L. Wang and S.Q. Liao
Aptamers that recognize the IgG Fc region are of great interest because of their wide application as an immunology probing tool, for diagnostics, and as affinity agents for antibody purification. We developed a target replacement strategy as a modification of conventional Systematic Evolution of Ligands by EXponential enrichment (SELEX) in order to effici.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.11
- DOI:
- 10.4238/2013.April.25.11
Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense
Authors: N.M.A. Rahman, H. Fu, H. Qiao, S. Jin, H. Bai, W. Zhang, F.W. Jiang, G. Liang, S. Sun, Y. Gong, F.F. Jiang, Y. Xiong and Y. Wu
Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different deve.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027950
- DOI:
- 10.4238/gmr.15027950