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Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum
Authors: M.B. Oliveira, S.C. Barbosa and S. Petrofeza
Dry bean (Phaseolus vulgaris L.) is an important economic crop in Brazil but its yield can be significantly reduced by white mold, a disease caused by Sclerotinia sclerotiorum (Lib.) de Bary, a necrotrophic, highly destructive, and non-host-specific fungus. This fungus secretes numerous cell wall-degrading enzymes such as polygalacturonases, exo-β-1,.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.6.3
- DOI:
- 10.4238/2013.June.6.3
Transferability of retrotransposon primers derived from Persimmon (Diospyros kaki Thunb.) across other plant species
Authors: X.Y. Du, Q.N. Hu, Q.L. Zhang, Y.B. Wang and Z.R. Luo
Retrotransposon-based molecular markers are powerful molecular tools. However, these markers are not readily available due to the difficulty in obtaining species-specific retrotransposon primers. Although recent techniques enabling the rapid isolation of retrotransposon sequences have facilitated primer development, this process nonetheless remains time-c.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.6.2
- DOI:
- 10.4238/2013.June.6.2
Identification and isolation of full-length cDNA sequences by sequencing and analysis of expressed sequence tags from guarana (Paullinia cupana)
Authors: L.C. Figueir�ªdo, A.C. Faria-Campos, S. Astolfi-Filho and J.L. Azevedo
The current intense production of biological data, generated by sequencing techniques, has created an ever-growing volume of unanalyzed data. We reevaluated data produced by the guarana (Paullinia cupana) transcriptome sequencing project to identify cDNA clones with complete coding sequences (full-length clones) and complete s.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1124
- DOI:
- 10.4238/vol10-2gmr1124
Homology-based cloning and expression analysis of Rf genes encoding PPR-containing proteins in tobacco
Authors: A.M. Ding, F.X. Li, Y.Q. Chen,, P. Zong, X. Qu, D.P. Gong, G.S. Liu and Y.H. Sun
As a model plant, mechanisms of the cytoplasmic male sterility/restoration of fertility (CMS/Rf) system in tobacco are seldom studied. Using Rf gene sequences from other Solanaceae plants and the draft genome of Nicotiana benthamiana, degenerate primers were designed to amplify the cDNA pool of N. tomentosiformis. In total, si.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.31.11
- DOI:
- 10.4238/2014.March.31.11
Genetic and morphological diversity of Moenkhausia oligolepis (Characiformes: Characidae) populations in the tributaries of the Araguaia River, Brazil: implications for taxonomy and conservation
Authors: T.J. Domingos, L.N. Moraes, R.M. Moresco, V.P. Margarido and P.C. Venere
Molecular genetic assessments that consider ecological information, in addition to endogamy levels, genetic diversity, and the genetic differentiation among species and populations, are particularly important for the conservation of biological diversity. Prime candidates for conservation genetic review are those subject to hum.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.11
- DOI:
- 10.4238/2014.September.29.11
Association study between matrix metalloproteinase-9 gene (MMP9) polymorphisms and the risk of Henoch-Sch�¶nlein purpura in children
Authors: E.D. Xu, Y.F. Xiao, J.J. Wang and L. Dong
Henoch-Schönlein purpura nephritis (HSPN), the most serious long-term complication of Henoch-Schönlein purpura, is one of the most common renal diseases in children. Matrix metalloproteinase-9 (MMP-9) is implicated in the pathogenesis of renal diseases. Genomic DNA was isolated from the venous blood leukocytes of 220.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028095
- DOI:
- 10.4238/gmr.15028095
Study of human acellular amniotic membrane loading bone marrow mesenchymal stem cells in repair of articular cartilage defect in rabbits
Authors: P.-F. Liu, L. Guo, D.-W. Zhao, Z.-J. Zhang, K. Kang, R.-P. Zhu and X.-L. Yuan
The aim of this study was to investigate the repair effect of human acellular amniotic membrane (HAAM) loading bone marrow mesenchymal stem cells (BMSCs) on articular cartilage defect in rabbits. Rabbit BMSCs were isolated and cultured, and they were then inoculated on HAAM to prepare the complex of HAAM and BMSCs. Twenty-four.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.12
- DOI:
- 10.4238/2014.September.29.12
Identification of novel DYNC2H1 mutations associated with short rib-polydactyly syndrome type III using next-generation panel sequencing
Authors: L.S. Chen, S.J. Shi, P.S. Zou, M. Ma, X.H. Chen and D.H. Cao
Short rib-polydactyly syndrome type III (SRPS3) is a perinatal lethal skeletal disorder with polydactyly and multisystem organ abnormalities. While ultrasound of the fetus can detect skeletal abnormalities characteristic of SRPS3, the syndrome is often difficult to diagnose before birth. As SRPS3 is an autosomal recessive diso.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028134
- DOI:
- 10.4238/gmr.15028134
Genetic variability and resistance of cultivars of cowpea [Vigna unguiculata (L.) Walp] to cowpea weevil (Callosobruchus maculatus Fabr.)
Authors: M.X. Vila Nova , N.G.A. Leite , L.M. Houllo , L.V. Medeiros, A.C. Lira Neto , B.S. Hsie1, L.R. Borges-Paluch , B.S. Santos, C.S.F. Araujo, A.A. Rocha and A.F. Costa
The cowpea weevil (Callosobruchus maculatus Fabr.) is the most destructive pest of the cowpea bean; it reduces seed quality. To control this pest, resistance testing combined with genetic analysis using molecular markers has been widely applied in research. Among the markers that show reliable results, the inter-simple sequenc.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.31.12
- DOI:
- 10.4238/2014.March.31.12
Downregulation of MACC1 expression enhances cisplatin sensitivity in SKOV-3/DDP cells
Authors: Z.M. Chen, H.R. Shi, X. Li, Y.X. Deng and R.T. Zhang
The aim of this study was to investigate the correlation between MACC1 expression and resistance to cisplatin (DDP) in DDP-resistant human epithelial ovarian cancer SKOV-3 cells (SKOV-3/DDP). MACC1 mRNA and protein expression levels in SKOV-3 and SKOV-3/DDP cells were detected by reverse transcriptase polymerase chain reaction and western blot. The SKOV-3.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.13
- DOI:
- 10.4238/2015.December.16.13