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cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca)
Authors: Y. Song, Y.-L. Hou, W.-R. Hou, G.-F. Wu and T. Zhang
The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherich.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1000
- DOI:
- 10.4238/vol10-1gmr1000
Characteristics of mature wheat embryos with different resistance to scab cultured in vitro with Fusarium graminearum crude toxin
Authors: S. Wang, M. Wang and C.Y. Zhang
Five wheat varieties with different resistance to scab were used to study the effect of Fusarium graminearum crude toxin on culture characteristics of mature wheat embryos in vitro. The results showed that the rate of induction and differentiation of mature embryo callus differed significantly between varieties, toxin concentration, and concentration x va.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.3
- DOI:
- 10.4238/2015.December.21.3
Polymorphisms in the promoter regions of the CXCL1 and CXCL2 genes contribute to increased risk of alopecia areata in the Korean population
Authors: S.K. Kim, J-H. Chung, H.J. Park, S.W. Kang, D.-J. Lim, S.H. Byun, D.G. Baek, H.Y. Ko, B.-L. Lew, H.H. Baik and W.-Y. Sim
Alopecia areata (AA) is a common disease, which causes hair loss in humans. AA has a genetically complex inheritance. This study investigated the possible correlations between single nucleotide polymorphisms (SNPs) in the promoter regions of the chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) and chemokine (C-X-C mot.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.29
- DOI:
- 10.4238/2015.August.14.29
Investigation into the association between NLRP3 gene polymorphisms and susceptibility to type 2 diabetes mellitus
Authors: S. Wang, F. Fang, W.B. Jin, X. Wang and X.S. Zheng
We conducted a case-control study to investigate the role of three common polymorphisms (rs10754558, rs7512998, and rs12137901) of the gene NLR family, pyrin domain containing 3 (NLRP3) in the development of type 2 diabetes mellitus (T2DM). Between May 2013 and May 2014, 385 patients with T2DM and 401 control subjects were enrolled in our study. Genotypin.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.15
- DOI:
- 10.4238/2015.December.21.15
Akirin2 expression in response to vaccine-induced immunity in chicken
Authors: C.L. Man, W.T. Mu, Y. Chang and D.X. Zhao
Akirin2 is a nuclear factor that plays an important role in the development and regulation of innate immune response. In this study, akirin2 gene expression in several primary immune organs (liver, thymus, and bursa) of Hi-Line Brown chicken administered with the LoSota vaccine was analyzed during the various stages of increase in Newcastle disease virus .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.20
- DOI:
- 10.4238/2015.December.21.20
Treating congenital megacolon by transplanting GDNF and GFR�±-1 double genetically modified rat bone marrow mesenchymal stem cells
Authors: C.B. Zhou, C.H. Peng, W.B. Pang, D. Zhang and Y.J. Chen
We studied the survival and gene expression of glial cell line-derived neurotrophic factor (GDNF) and GDNF receptor a-1 (GFRa-1) double-genetically modified rat bone marrow mesenchymal stem cells (BMSCs) transplanted into the intestinal walls of the rat models with congenital megacolon and determine the feasibility of treatment by transplantation of doubl.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.8
- DOI:
- 10.4238/2015.August.14.8
Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm
Authors: R.S. Celestino, M.I. Zucchi, J.B. Pinheiro, J.B. Campos, A.A. Pereira, F.G. Bianchini, R.N. Lima, M.F. Arrigoni-Blank, P.B. Alves and A.F. Blank
Due to the economic interests in vetiver, Chrysopogon zizanioides (L.) Roberty, molecular and chemical studies are essential to generate information for its sustainable exploitation. The aim of this study was to undertake a molecular and chemical characterization of vetiver accessions of the active germplasm bank of the Universidade Federal de Sergipe. Th.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.9
- DOI:
- 10.4238/2015.August.14.9
N-ethylmaleimide-sensitive factor siRNA improves cardiac function following myocardial infarction in rats
Authors: Y. Zhou , Y. Liu, S.X. Yang and Z. Wang
This study examined the effects of N-ethylmaleimide-sensitive factor (NSF) small interfering RNA (siRNA) on cardiac function following myocardial infarction (MI) in rats. Thirty-six adult Sprague Dawley rats were randomly divided into three equivalent groups. An acute MI model was established by ligating the anterior descending branch of the left coronary.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.11
- DOI:
- 10.4238/2015.August.14.11
Overexpression of an endo-1,4-�²-glucanase V gene (EGV) from Trichoderma reesei leads to the accumulation of cellulase activity in transgenic rice
Authors: X.Y. Li, F. Liu, Y.F. Hu, M. Xia, B.J. Cheng, S.W. Zhu and Q. Ma
The ectopic expression of cellulase in biomass can reduce the cost of biofuel conversion. This trait modification technique is highly beneficial for biofuel production. In this study, we isolated an endo-1,4-beta-glucanase gene (EGV) from Trichoderma reesei and inserted this gene downstream of a fragment encoding the signal peptide Apo-SP in a modified pC.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.24
- DOI:
- 10.4238/2015.December.21.24
Aldehyde dehydrogenase 2 protects human umbilical vein endothelial cells against oxidative damage and increases endothelial nitric oxide production to reverse nitroglycerin tolerance
Authors: X.Y. Hu, Q. Fang, D. Ma, L. Jiang, Y. Yang, J. Sun, C. Yang and J.S. Wang
Medical nitroglycerin (glyceryl trinitrate, GTN) use is limited principally by tolerance typified by a decrease in nitric oxide (NO) produced by biotransformation. Such tolerance may lead to endothelial dysfunction by inducing oxidative stress. In vivo studies have demonstrated that aldehyde dehydrogenase 2 (ALDH2) plays impor.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027822
- DOI:
- 10.4238/gmr.15027822