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Enzymatic differences between the endophyte Guignardia mangiferae (Botryosphaeriaceae) and the citrus pathogen G. citricarpa
Authors: A.S. Rom�£o, M.B. Sp�³sito, F.D. Andreote, J.L. Azevedo and W.L. Ara�ºjo
The endophyte Guignardia mangiferae is closely related to G. citricarpa, the causal agent of citrus black spot; for many years these species had been confused with each other. The development of molecular analytical methods has allowed differentiation of the pathogen G. citricarpa from the endophyte G. mangiferae, but the phys.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr952
- DOI:
- 10.4238/vol10-1gmr952
Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil
Authors: Q.L. Barcelos, E.A. Souza and K.J. Damasceno e Silva
The causal agent of common bean anthracnose, Colletotrichum lindemuthianum, has considerable genetic and pathogenic variability, which makes the development of resistant cultivars difficult. We examined variability within and between Brazilian pathotypes of C. lindemuthianum through the identification of vegetative compatibili.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr907
- DOI:
- 10.4238/vol10-1gmr907
Expression of recombinant human anti-TNF-�± scFv-Fc in Arabidopsis thaliana seeds
Authors: N. Yao, L. Ai, Y.Y. Dong, X.M. Liu, D.Z. Wang, N. Wang, X.W. Li,F.W. Wang, XK. Li, H.Y. Li and C. Jiang
Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest si.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027726
- DOI:
- 10.4238/gmr.15027726
Polymorphisms of GSTM1, GSTT1, and p53 in Goi�¢nia, Goi�¡s
Authors: D.A. Rodrigues, I.R. Costa, J.V.M. Martins, K.S.F. e Silva and K.K.V.O. Moura
Genetic polymorphisms are defined as changes within the DNA sequences of genes that have frequencies in the population higher than 1%. The glutathione S-transferases play an important role in the cellular detoxification systems involved in oxidative stress that can lead to accumulation of reactive oxygen species. Epidemiological studies have suggested tha.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.4
- DOI:
- 10.4238/2015.December.21.4
Isolation of genomic DNA from defatted oil seed residue of rapeseed (Brassica napus)
Authors: M. Sadia, M.A. Rabban, S. Hameed, S.R. Pearce and S.A. Malik
A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil-free seed residue of Brassica napus, an important oil seed plant, has been developed. Oil from the dry seeds was completely recovered in an organic solvent and quantified gravimetrically followed by processing of the residual bioma.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1009
- DOI:
- 10.4238/vol10-1gmr1009
Genomic ancestry evaluated by ancestry-informative markers in patients with sickle cell disease
Authors: A.F. Nascimento, J.S. Oliveira2, J.C. Silva Junior and A.A.L. Barbosa
The bs mutation is responsible for the most aggressive form of sickle cell disease, has a predominantly African origin, and arrived in Brazil through the slave trade. However, the Brazilian population is highly miscegenated, underscoring the importance of ancestry-informative markers (AIMs) for the identification of the geneti.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017604
- DOI:
- 10.4238/gmr.15017604
Critical genes in head and neck squamous cell carcinoma revealed by bioinformatic analysis of gene expression data
Authors: B. Wang, T. Wang, X.L. Cao and Y. Li
In this study, bioinformatic analysis of gene expression data of head and neck squamous cell carcinoma (HNSCC) was performed to identify critical genes. Gene expression data of HNSCC were downloaded from the Cancer Genome Atlas (TCGA) and differentially expressed genes were determined through significance analysis of microarrays. Protein-protein interacti.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.10
- DOI:
- 10.4238/2015.December.21.10
Three novel SNPs of the bovine Tf gene in Chinese native cattle and their associations with milk production traits
Authors: Z.H. Ju, Q.L. Li, J.M. Huang, M.H. Hou, R.L. Li, J.B. Li, J.F. Zhong and C.F. Wang
Transferrin (Tf) is a β-globulin protein that transports iron ions in mammalian cells. It contributes to innate immunity to microbial pathogens, primarily by limiting microbial access to iron. Thus, polymorphisms present in bovine Tf could potentially underlie inherited differences in mastitis resistance and milk producti.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1038
- DOI:
- 10.4238/vol10-1gmr1038
Influence of vascular endothelial growth factor and alpha-fetoprotein on hepatocellular carcinoma
Authors: E.Y. Yvamoto, R.F. Ferreira, V. Nogueira, M.A.S. Pinhel, G.D. Tenani, J.G.S.C. Andrade, M.E.L. Baitello, M.L. Greg�³rio, P.S. Fucuta, R.F. Silva, D.R.S. Souza and R.C.M.A. Silva
We evaluated the influence of the vascular endothelial growth factor (VEGF) -C936T polymorphism on prognosis of hepatocellular carcinoma (HCC), cirrhosis, and hepatitis C virus (HCV) infection. Serum VEGF and alpha-fetoprotein (AFP) levels were determined and used to characterize sensitivity and specificity. A total of 285 subjects were studied: 68 HCC, 1.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.16
- DOI:
- 10.4238/2015.December.21.16
Sequence variations in the FAD2 gene in seeded pumpkins
Authors: Y. Ge, Y. Chang, W.L. Xu, C.S. Cui and S.P. Qu
Seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. The fatty acid desaturase-2 (FAD2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. However, little is known about sequence variations in FAD2 .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.19
- DOI:
- 10.4238/2015.December.21.19