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Effects of cartilage-derived morphogenetic protein 1 (CDMP1) transgenic mesenchymal stem cell sheets in repairing rabbit cartilage defects
Authors: Y. Cui, M. Yao, Y. Liu, L. Mu, B. Zhang and G. Wu
The aim of this study was to investigate the abilities of cartilage-derived morphogenetic protein 1 (CDMP1) transgenic cell sheets in repairing rabbit cartilage defects. Rabbit CDMP1 transgenic bone marrow mesenchymal stem cell (BMSC) sheets (CDMP1-BMSCs) were cultured on temperature-sensitive culture dishes, and CDMP1 expression and type II collagen prot.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028058
- DOI:
- 10.4238/gmr.15028058
Development of microsatellite markers in Hagenia abyssinica (Bruce) J.F. Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing
Authors: A.W. Gichira, Z.C. Long, G.W. Hu, R.W. Gituru, Q.F. Wang and J.M. Chen
Hagenia abyssinica (Bruce) J.F. Gmel is an endangered tree species endemic to the high mountains of tropical Africa. We used Illumina paired-end technology to sequence its nuclear genome, aiming at creating the first genomic data library and developing the first set of genomic microsatellites. Seventeen microsatellite markers were validated in 24 individu.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028168
- DOI:
- 10.4238/gmr.15028168
Persistent glucocorticoid resistance in systemic lupus erythematosus patients during clinical remission
Authors: A.K.G. Melo, M.R. Melo, A.B.A. Saramago, G. Demartino, B.D.B. Souza and C.A. Longui
Glucocorticoids (GCs) are key drugs in the treatment of systemic lupus erythematosus (SLE). GC dose reduction during remission is related to disease activity, GC dose used, length of treatment, and individual GC sensitivity. We compared GC receptor α (GRα) isoform and nuclear factor kappaB (NF-κB) messenger RNA quantitation and in vivo G.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.February.19.1
- DOI:
- 10.4238/2013.February.19.1
Correlations between traits in soybean (Glycine max L.) naturally infected with Asian rust (Phakopsora pachyrhizi)
Authors: B. Rodrigues, F. Serafim, A.P.O. Nogueira, O.T. Hamawaki, L.B. de Sousa and R.L. Hamawaki
Soybean (Glycine max L.)-breeding programs aim to develop cultivars with high grain yields and high tolerance to Asian soybean rust (Phakopsora pachyrhizi). Considering that the traits targeted for breeding are mainly quantitative in nature, knowledge of associations between traits allows the breeder to formulate indirect selection strategies. In this stu.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.45
- DOI:
- 10.4238/2015.December.21.45
Cobalt chloride induces metaphase when topically applied to larvae and pupae of the stingless bee Melipona scutellaris (Hymenoptera, Apidae, Meliponini)
Authors: C. Ueira-Vieira, R.R. Tavares, S. Morelli, B.B. Pereira, R.P. Silva, A.R. Torres-Mariano, W.E. Kerr and A.M. Bonetti
In order to optimize preparations of bee metaphases, we tested cobalt chloride, which has been used as a metaphase inducer in other organisms, such as hamsters and fish. Four microliters of 65 mM cobalt chloride aqueous solution was topically applied to larval and pupal stages of the stingless bee Melipona scutellaris. The cerebral ganglion was removed af.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.February.6.1
- DOI:
- 10.4238/2013.February.6.1
A network approach predicts NFKBIA and BIRC3 as pathogenic genes in childhood asthma
Authors: X.M. Gao
This study aimed to predict pathogenic genes of childhood asthma based on molecular interaction networks and gene expression data. Known pathogenic genes identified from the human protein-protein interaction network were denoted as seed genes, and were included in network A. We extracted sub-network B (pathogenic network), whi.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028226
- DOI:
- 10.4238/gmr.15028226
One missense mutation in exon 2 of the PAX5 gene in Iran
Authors: S. Yazdanparast, S.R. Khatami, H. Galehdari and K. Jaseb
The PAX5 gene, which encodes the B-cell specific activator protein, is one of the most important factors in determination of B-cell development. This gene is the main target of somatic mutations in acute B lymphoblastic leukemia (B-ALL). For example, point mutations, deletions, as well as other gene rearrangements may lead to several forms of B-cell malig.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.1
- DOI:
- 10.4238/2015.December.22.1
Effects of prolonged anesthesia with dexmedetomidine, fentanyl, or remifentanil on the self-renewal of mouse embryonic stem cells
Authors: N. Zhang, Y.R. Cai, X.W. Yi, Y.N. Xiao, B. Chen and W.X. Li
Previous study has indicated that exposure to anesthesia in early development leads to neuro-apoptosis and is followed by long-term cognitive dysfunction. Given that larger numbers of pregnant women currently receive anesthesia during the first trimester, we wanted to mimic this process in vitro using mouse embryonic stem cells (mESCs) and to explore how .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.5
- DOI:
- 10.4238/2015.December.22.5
Lack of association between IL-10 -1082G/A polymorphism and chronic periodontal disease in adults
Authors: R. Hannum, F.R. Godoy , A.S. da Cruz, T.C. Vieira, L.B. Minasi, D. de M. e Silva, C.C. da Silva and A.D. da Cruz
Because of the complex interaction between periodontal pathogens and the host defense system, periodontitis is considered an inflammatory disorder of bacterial etiology that results in periodontal tissue damage. Genetic mechanisms may interfere with the gene expression of important inflammation mediators, modulating the immunologic response of an individu.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.7
- DOI:
- 10.4238/2015.December.22.7
Detection of soluble expression and in vivo interactions of the inner membrane protein OppC using green fluorescent protein
Authors: Q.J. Xiang, J.F. Zhai, M. Zhang and B. Zhang
In this study, the in vivo interaction system of oligopeptide permease (Opp) proteins was analyzed, and a high expression system of inner membrane protein OppC was constructed by flexible usage of the green fluorescent protein (GFP). The Escherichia coli OppC gene, which encodes a transmembrane component of oligopeptide transporter, was cloned into differ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.8
- DOI:
- 10.4238/2015.December.22.8