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PKR and HMGB1 expression and function in rheumatoid arthritis
Authors: W.J. Wang, S.J. Yin and R.Q. Rong
The pathogenesis of rheumatoid arthritis (RA) is characterized by inflammation. We aimed to examine the roles of double-stranded RNA-activated protein kinase (PKR) and high-mobility group box chromosomal protein 1 (HMGB1) in a rat model of RA. Male SD rats were divided into three groups: control, RA model, and intervention (RA model plus treatment). The m.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.11
- DOI:
- 10.4238/2015.December.22.11
Protective efficacy of a genetic subunit bacterin against edema disease of swine in mice
Authors: X.L. Yang, Y.J. Liu, L.Q. Hu, L.W. Guo, G.P. Liu, B. Wu and H.C. Chen
The exotoxin SLT-IIeB from the Escherichia coli Ee strain was expressed in E. coli, and the recombinant protein was purified, mixed with the Ee strain, then emulsified with oil-emulsion adjuvants to obtain a mixed subunit bacterin. Groups of Kunming mice were immunized at weeks 0 and 2, and challenged intraperitoneally with the Ee strain at week 4. Antibo.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.29
- DOI:
- 10.4238/2015.December.22.29
Sevoflurane induces neurotoxicity in young mice through FAS/FASL signaling
Authors: Q. Song, Y.L. Ma, J.Q. Song, Q. Chen, G.S. Xia, J.Y. Ma, F. Feng, X.J. Fei and Q.M. Wang
Sevoflurane, the most widely used anesthetic in clinical practice, has been shown to induce apoptosis, inhibit neurogenesis, and cause learning and memory impairment in young mice. However, the underlying mechanism is still unknown. In this study, wild-type and the FAS- or FAS ligand (FASL)-knockout mice (age 7 days) were exposed to sevoflurane or pure ox.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.32
- DOI:
- 10.4238/2015.December.22.32
Genetic variant screening of MC3R and MC4R genes in early-onset obese children and their relatives among a Thai population: family-based study
Authors: S. Wannaiampikul, B. Phonrat, A. Tungtrongchitr, C. Limwongse, N. Chongviriyaphan, J. Santiprabhob and R. Tungtrongchitr
MC3R (melanocortin-3 receptor) and MC4R (melanocortin-4 receptor) play important roles in energy homeostasis. Severe early-onset obesity, known as monogenic obesity when it is the consequence of a mutation in a single-gene product, may result when energy homeostasis is disrupted. The purpose of our study was to screen for variations of the MC3R and MC4R g.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.35
- DOI:
- 10.4238/2015.December.22.35
Cloning and characterization of the nicotianamine synthase gene in Eruca vesicaria subsp sativa
Authors: B.L. Huang, C. Cheng, G.Y. Zhang, J.J. Su, Y. Zhi, S.S. Xu, D.T. Cai, X.K. Zhang and B.Q. Huang
Nicotianamine (NA) is a ubiquitous metabolite in plants that bind heavy metals, is crucial for metal homeostasis, and is also an important metal chelator that facilitates long-distance metal transport and sequestration. NA synthesis is catalyzed by the enzyme nicotianamine synthase (NAS). Eruca vesicaria subsp sativa is highly tolerant to Ni, Pb, and Zn. .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.38
- DOI:
- 10.4238/2015.December.22.38
Effects of different dwarfing interstocks on key enzyme activities and the expression of genes related to malic acid metabolism in Red Fuji apples
Authors: J. Shi, F.F. Li, H. Ma, Z.Y. Li and J.Z. Xu
In this experiment, the test materials were ‘Red Fuji’ apple trees grafted onto three interstocks (No. 53, No. 111, and No. 236), which were chosen from SH40 seeding interstocks. The content of malic acid, the enzyme activities, and the expression of genes related to malic acid metabolism were determined during fruit development.The results sh.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.40
- DOI:
- 10.4238/2015.December.21.40
Mesenchymal stem cells from skin lesions of psoriasis patients promote proliferation and inhibit apoptosis of HaCaT cells
Authors: R.F. Liu, F. Wang, Q. Wang, X.C. Zhao and K.M. Zhang
Psoriasis is an inflammatory skin disease characterized by excessive proliferation and abnormal differentiation and apoptosis of keratinocytes (KCs). Mesenchymal stem cells (MSCs) from skin lesions of psoriasis patients demonstrate abnormal cytokine secretion, which may affect KC proliferation and apoptosis. Here, we explored how MSCs from skin lesions of.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.49
- DOI:
- 10.4238/2015.December.21.49
Role of heme oxygenase-1 in demethylating effects on SKM-1 cells induced by decitabine
Authors: R. Gao, D. Ma, P. Wang, J. Sun, J.S. Wang and Q. Fang
We evaluated the influence of heme oxygenase-1 (HO-1) gene inhibition in myelodysplastic syndrome (MDS) cell line SKM-1 on enhancement of the demethylating effects of decitabine on p15, and explored the possible mechanism. DNMT1 gene expression in SKM-1 cells was silenced by being transfected by a constructed siRNA with liposomes. The proliferation inhibi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.3
- DOI:
- 10.4238/2015.December.22.3
Genetic diversity and variability in populations of the white wax insect Ericerus pela, assessed by AFLP analysis
Authors: H. Chen, R. He, Z.L. Wang, S.Y. Wang, Y. Chen, Z.C. Zhu and X.M. Chen1
The white wax insect Ericerus pela Chavannes (Hemiptera: Coccoidea) is an economically valuable insect species that has been used for over a thousand years in China. The present study focuses on assessing the genetic variability in different populations of E. pela collected from seven Chinese provinces. The amplified fragment length polymorphism technique.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.6
- DOI:
- 10.4238/2015.December.22.6
Quantitative trait locus analysis of leaf morphological characters, yield-related traits, and secondary metabolite contents in Eucommia ulmoides
Authors: Y. Li, J.K. Wei, Z.Q. Li, A.N. Lei and M.H. Liu
Eucommia ulmoides is cultivated for the production of eucommia rubber and Chinese herbal drugs. Molecular breeding methods, such as marker-assisted selection (MAS), have the potential to improve the efficiency of E. ulmoides breeding. Quantitative trait locus (QTL) analysis was applied to identify marker-trait associations for E. ulmoides using an F1 mapp.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.12
- DOI:
- 10.4238/2015.December.22.12