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Microbial Genetics   Research Article

Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana

Authors: W.Y. Xuan, Y. Zhang, Z.Q. Liu, D. Feng and M.Y. Luo

Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. m.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.27
DOI:
10.4238/2015.August.19.27
Human Genetics   Research Article

Association of TNF-�±, CTLA4, and PTPN22 polymorphisms with type 1 diabetes and other autoimmune diseases in Brazil

Authors: N.A.C. Tavares, M.M.S. Santos, R. Moura, J. Ara�ºjo, R.L. Guimar�£es, S. Crovella and L.A.C. Brand�£o

Type 1 diabetes mellitus (T1D) is a complex disorder characterized by an autoimmune response against human pancreatic beta-cells. Patients with T1D can also develop a response toward one or more other factors, such as in autoimmune thyroiditis (AITD) and celiac disease (CD). In the presence of T1D + AITD, the patient is diagnosed with autoimmune polygland.. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.42
DOI:
10.4238/2015.December.28.42
Medical Genetics   Research Article

Genetic diversity analysis of sweet kernel apricot in China based on SSR and ISSR markers

Authors: M.P. Liu, H.Y. Du, G.P. Zhu, D.L. Fu and W.Y. Tana

Simple sequence repeat (SSR) and inter-simple sequence repeat (ISSR) markers were used to evaluate genetic diversity among 22 sweet kernel apricot accessions and 12 cultivars in China to provide information on how to improve the utilization of kernel apricot germplasms. The results showed that 10 pairs of SSR primers screened from 40 primer pairs amplifie.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.4
DOI:
10.4238/2015.August.19.4
Medical Genetics   Research Article

Identification of conservation units of the hynobiid salamander Pachyhynobius shangchengensis

Authors: L.-N. Su, Y.-Y. Zhao, X.-M. Wu, H.-F. Zhang and X.-C. Li

The evolutionary significant units (ESUs) of the salamander Pachyhynobius shangchengensis (Hynobiidae) in the Dabieshan mountains, southeastern China, were identified based on mitochondrial DNA data. We used methods for detecting cryptic species, such as the minimum spanning tree, the automatic barcode gap discovery, and the generalized mixed Yule-coalesc.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.10
DOI:
10.4238/2015.August.19.10
Human Genetics   Research Article

APOA5 -1131T>C and APOC3 -455T>C polymorphisms are associated with an increased risk of coronary heart disease

Authors: Y. Sun, R.B. Zhou and D.M. Chen

The aim of this study was to investigate correlations between apolipoprotein A-V (APOA5) -1131T>C and apolipoprotein C-III (APOC3) -455T>C polymorphisms and coronary heart disease (CHD). PubMed, Ovid, Cochrane Library, Embase, China National Knowledge Infrastructure, and Wanfang databases were searched using combinations of keywords relating to thes.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.9
DOI:
10.4238/2015.December.23.9
Animal Genetics   Research Article

Expression pattern of JMJD1C in oocytes and its impact on early embryonic development

Authors: C.H. Li, Y. Gao, S. Wang, F.F. Xu, L.S. Dai, H. Jiang, X.F. Yu, C.Z. Chen, B. Yuan and J.B. Zhang

Cell reprogramming mediated by histone methylation and demethylation is crucial for the activation of the embryonic genome in early embryonic development. In this study, we employed quantitative real-time polymerase chain reaction (qRT-PCR) to detect mRNA levels and expression patterns of all known histone demethylases in early germinal vesicle stage and .. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.12
DOI:
10.4238/2015.December.23.12
Animal Genetics   Research Article

Effect of Ginkgo biloba extract on matrix metalloproteinase-3 expression in a rat model of chondrocyte injury

Authors: J.Z. Zeng, L.F. Ma, H. Meng, H.M. Yu, Y.K. Zhang and A. Guo

A rat model with cartilage chondrocyte injury was established using interleukin-1β (IL-1β) to investigate the effect of Ginkgo biloba extract (EGb) on matrix metalloproteinase-3 (MMP-3) expression. Rat chondrocytes were extracted and randomly divided into six groups: control group, IL-1β (model) group, IL-1β + dexamethasone group, and .. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.15
DOI:
10.4238/2015.December.23.15
Microbial Genetics   Research Article

Cloning and characterization of ADP-glucose pyrophosphorylase small subunit gene in Cyperus esculentus (yellow nutsedge)

Authors: C. Cheng, J. Hu, Y. Zhi, J.J. Su, X.K. Zhang and B.Q. Huang

ADP-glucose pyrophosphorylase (ADPGlcPPase) controls the first committed step of starch synthesis by catalyzing the biosynthesis of ADP-glucose from glucose-phosphate and ATP. It is a tetrameric protein consisting of two small and two large subunits. The small subunits have a catalytic function, while the large subunits regulate the enzyme activity. Cyper.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.18
DOI:
10.4238/2015.December.23.18
Animal Genetics   Research Article

Identification of androgenic gland microRNA and their target genes to discover sex-related microRNA in the oriental river prawn, Macrobrachium nipponense

Authors: S.B. Jin, H.T. Fu, S.F. Jiang, Y.W. Xiong, H. Qiao, W.Y. Zhang, Y.S. Gong and Y. Wu

The oriental river prawn, Macrobrachium nipponense, is an important aquaculture species in China. The androgenic gland produces hormones that play crucial roles in the differentiation of crustaceans to the male sex. MicroRNA (miRNA) post-transcriptionally regulates many protein-coding genes, influencing important biological and metabolic processes. Howeve.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.27
DOI:
10.4238/2015.December.23.27
Plant Genetics   Research Article

Comparison of BLUE and BLUP/REML in the selection of clones and families of potato (Solanum tuberosum)

Authors: C.A. Ticona-Benavente and D.F. da Silva Filho

The use of best linear unbiased prediction/restricted maximum likelihood (BLUP/REML) in perennial crops and animal breeding enhances selection gain. However, its advantage with respect to annual crops is not clear. We compared the BLUP and best linear unbiased estimator selection efficiency in the breeding of various potato generations. This was done by s.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.30
DOI:
10.4238/2015.December.23.30