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Microbial Genetics   Research Article

Isolation and characterization of microsatellite loci in Quercus fabri (Fagaceae)

Authors: Z.Z. Xiao, W.W. Chen, W. Bao, R. Wang and Y.Y. Li

Quercus fabri is a pioneer species of secondary succession in evergreen broadleaved forests in China. In this study, we isolated and developed 12 polymorphic and 2 monomorphic microsatellite loci for Q. fabri using the biotin-streptavidin capture method. We characterized 12 polymorphic loci in 52 individuals from two populatio.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028322
DOI:
10.4238/gmr.15028322
Human Genetics   Research Article

Operational taxonomic units (OTUs) of endophytic bacteria isolated from banana cultivars in the Amazon

Authors: ?. Souza, V.B. Nogueira, J.C. Cruz, N.R. Sousa, A.R.L. Proc?³pio and G.F. Silva

Endophytic microorganisms colonize plants, inhibit the growth of pathogens (by competing for nutrients and/or space), or produce antagonistic substances. Fifty-five endophytic bacteria were isolated from the leaf tissue of the FHIA 18 banana cultivar. Genetic diversity analyses were performed using the enterobacterial repetitive intergenic consensus seque.. Read More»

Genet. Mol. Res. 14(3):
2015.August.19.28
DOI:
10.4238/2015.August.19.28
Human Genetics   Research Article

Association between ARNTL (BMAL1) rs2278749 polymorphism T >C and susceptibility to Alzheimer disease in a Chinese population

Authors: Q. Chen, X.D. Peng, C.Q. Huang, X.Y. Hu and X.M. Zhang

In the present study, we examined whether the ARNTL (BMAL1) rs2278749 T/C polymorphism was associated with the susceptibility to Alzheimer disease (AD). This case-control study examined the genotypes of apolipoprotein E (APOE e4) and BMAL1 rs2278749 T/C using restriction fragment length polymorphism and the TaqMan assay, respectively. A total of 296 unrel.. Read More»

Genet. Mol. Res. 14(4):
2015.December.23.39
DOI:
10.4238/2015.December.23.39
Human Genetics   Research Article

Sex- and age-dependent expression of Pax7, Myf 5, MyoG, and Myostatin in yak skeletal muscles

Authors: G. Wu, J. Zhang, L. Wang, S. Xu, J. Zhou, A. Xiang and C. Yang

The aim of this study was to investigate the myogenic factor mRNA expression pattern of Pax7, Myf5, MyoG, and Myostatin mRNA at different ages, sexes, and muscle tissues of Datong yaks. The expression patterns in semimembranosus (SM), quadriceps femoris (QF), and triceps muscle of arm (TM) were detected by quantitative real-ti.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028020
DOI:
10.4238/gmr.15028020
Plant Genetics   Research Article

In vitro embryo rescue culture of F1 progenies from crosses between different ploidy grapes

Authors: W. Ji, G.R. Li , Y.X. Luo, X.H. Ma, M. Wang and R. Ren

Crossing different ploidy grapes is an effective way to obtain new seedless cultivars. Although embryo rescue has been extensively applied in breeding seedless and triploid grapes, only a few improved cultivars have been developed. Based on preliminary studies, we set five crosses between tetraploid and diploid grape varieties to obtain new hybrid triploi.. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.10
DOI:
10.4238/2015.December.28.10
Animal Genetics   Research Article

Genetic analysis of five sedentary fish species in middle Laranjinha River (upper Paran?¡ River basin): A case study

Authors: W. Frantine-Silva, D.G. Ferreira, R.H.C. Nascimento, J.F. Fracasso, J.E. Conte, F.P. Ramos, S. Carvalho and B.A. Galindo

Most studies of diversity and genetic structure in neotropical fish have focused on commercial species from large rivers or their reservoirs. However, smaller tributaries have been identified as an important alternative migratory route, with independent pools of genetic diversity. In this context, the present study aimed to evaluate genetic diversity and .. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.13
DOI:
10.4238/2015.December.28.13
Human Genetics   Research Article

Evolutionary analysis of the ubiquitin gene of baculovirus and insect hosts

Authors: S.S. Ma, Z. Zhang, H.C. Xia, L. Chen, Y.H. Yang, Q. Yao and K.P. Chen

Baculovirus is the only virus that has been found to encode the ubiquitin protein. In this study, ubiquitin sequences from 16 insects and 49 viruses were collected and compared. The resulting sequences were aligned with virus genomes. Then MAGE 5.0, k-estimated software, as well as other software programs were used for systemic evolutionary, selection pre.. Read More»

Genet. Mol. Res. 14(3):
2015.August.21.2
DOI:
10.4238/2015.August.21.2
Microbial Genetics   Research Article

Association of the PPAR?³2 Pro12Ala polymorphism with increased risk of cardiovascular diseases

Authors: Y. Li, J. Zhu and J.Q. Ding

This meta-analysis investigated the correlation between the PPARγ2 Pro12Ala polymorphism and cardiovascular disease (CVD). Electronic database and manual searches were conducted to retrieve studies published relevant to the PPARγ2 Pro12Ala polymorphism and CVD. Rigorous inclusion and exclusion criteria were employed for selection of high-quali.. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.15
DOI:
10.4238/2015.December.28.15
Human Genetics   Research Article

Analysis of the relationship between peripheral blood T lymphocyte subsets and HCV RNA levels in patients with chronic hepatitis C.

Authors: Y. Huang, M.J. Zheng and Y.H. Xu

We investigated the relationship between peripheral blood T lymphocyte subsets and hepatitis C virus (HCV) RNA levels in patients with hepatitis C. Samples from 69 chronic hepatitis C (CHC) patients and 20 healthy controls were analyzed using quantitative polymerase chain reaction (PCR) to detect HCV RNA and flow cytometry to determine the expression leve.. Read More»

Genet. Mol. Res. 14(3):
2015.August.21.12
DOI:
10.4238/2015.August.21.12
Animal Genetics   Research Article

High polymorphism at microsatellite loci in the Chinese donkey

Authors: R.F. Zhang, W.M. Xie, T. Zhang and C.Z. Lei

To reveal the genetic diversity and phylogenetic relationships between Chinese donkey breeds, 415 individuals representing ten breeds were investigated using ten microsatellite markers. The observed number of alleles, mean effective number of alleles (NE), mean expected heterozygosity (HE), and polymorphic information content .. Read More»

Genet. Mol. Res. 15(2):
gmr.15028291
DOI:
10.4238/gmr.15028291